FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ DCP-RPMI-QPR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 formulation engineered for hematopoietic cells, lymphocytes, and related immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose | [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate.
- Glucose source: 2000 mg/L (2.0 g/L) D-glucose provides a defined, user-controlled carbon source
- Formulated without L-glutamine, sodium pyruvate, and phenol red for independent supplementation and reduced optical background in fluorescence assays
- Sodium bicarbonate (2.0 g/L) buffering system supports pH 7.4 under a 5% CO₂ atmosphere
- Glutathione (reduced, 1.0 mg/L) included — antioxidant support for redox-sensitive immune cell types
- Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
- Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>)
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- Microfluidics Suitable — engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) platforms
- Glucose2000 mg/L (2.0 g/L)
- Formulation[+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose | [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate
- AppearanceColorless to pale yellow, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)280 - 320 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack
Engineered where standard media fails
Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that can confound immune cell assays. FluxMPS™ is built to address these gaps.
Microchannel-safe purity
0.04 µm final filtration stage with USP <788> Method 1 particulate compliance. Ultra-low particulate counts support suspension immune cell culture, flow cytometry, and microfluidic OoC channels without clogging.
Total metabolic control
Formulated without L-glutamine and sodium pyruvate so researchers can define the carbon and nitrogen source independently — useful for lymphocyte metabolic flux and Warburg-effect studies in cancer cell lines.
Ultrapure-grade water
Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic carbon (TOC) content supports reproducible, low-background cell culture assays.
Low background for imaging
Phenol red–free formulation and ultra-low particulate background support confocal microscopy, biosensor readouts, and flow cytometry in immune cell assays.
Rich, stable nutrient profile
RPMI 1640 amino acid and vitamin profile, including glutathione (reduced) for antioxidant support, released under micro-batch, per-lot QC.
Customization on demand
pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish.
-
1
0.1 µm Prefiltration I
Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-RPMI-QPR1X
Immune cell OoC and hematopoietic applications
FluxMPS™ DCP-RPMI-QPR1X — RPMI 1640 — delivers 0.04 µm filtered purity for hematopoietic cells and related OoC applications.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available on request for automated, high-uptime systems.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
T Cell & Lymphocyte Culture
RPMI 1640 is the standard for primary T cells, B cells, NK cells, and monocytes. FluxMPS 0.04 µm filtration reduces particulate load in immune assays.
Leukemia & Lymphoma Lines
RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and hematopoietic cancer lines where DMEM would alter proliferation and signaling.
Immune Cell OoC
0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models.
CAR-T & TIL Expansion
Ultra-low endotoxin release specification (< 0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols where endotoxin control is critical.
Immune Cell Metabolic Flux
Defined RPMI base for glycolysis stress tests and 13C isotope tracing of lymphocyte activation states. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Flow Cytometry & Confocal
Ultra-low particulate background; phenol red–free formulation reduces optical interference for PE-channel flow cytometry and immune cell confocal imaging.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose | [-] L-Glutamine, [-] Phenol Red, [-] Sodium Pyruvate |
| Appearance | Colorless to pale yellow, clear solution |
| Glucose | 2000 mg/L (2.0 g/L) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 280 - 320 mOsm/kg H2O |
| Total ingredients | 38 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ required (bicarbonate-buffered, 2.0 g/L NaHCO3) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
RPMI 1640: 38 ingredients per lot with CAS numbers for full raw-material traceability. Contains glutathione (reduced) and a balanced amino acid and vitamin profile suited to lymphocyte and hematopoietic cell culture.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium nitrate tetrahydrate | 13477-34-4 | 100.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 48.840 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2000.000 |
| Sodium chloride | 7647-14-5 | 6000.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 800.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 10.000 |
| L-Arginine hydrochloride | 1119-34-2 | 241.000 |
| L-Asparagine | 70-47-3 | 50.000 |
| L-Aspartic acid | 56-84-8 | 20.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 65.200 |
| L-Glutamic acid | 56-86-0 | 20.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Hydroxyproline | 51-35-4 | 20.000 |
| L-Isoleucine | 73-32-5 | 50.000 |
| L-Leucine | 61-90-5 | 50.000 |
| L-Lysine hydrochloride | 657-27-2 | 40.000 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 15.000 |
| L-Proline | 147-85-3 | 20.000 |
| L-Serine | 56-45-1 | 30.000 |
| L-Threonine | 72-19-5 | 20.000 |
| L-Tryptophan | 73-22-3 | 5.000 |
| L-Tyrosine Disodium Salt | 69847-45-6 | 28.830 |
| L-Valine | 72-18-4 | 20.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 3.000 |
| D-Biotin | 58-85-5 | 0.200 |
| D-Ca-Pantothenate | 137-08-6 | 0.250 |
| Folic acid | 59-30-3 | 1.000 |
| Niacinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| Vitamin B12 | 68-19-9 | 0.005 |
| p-Amino benzoic acid (PABA) | 150-13-0 | 1.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Glutathione reduced | 70-18-8 | 1.000 |
| i-Inositol | 87-89-8 | 35.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016–certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm with controlled trace-metal and organic carbon (TOC) content.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — Certificate of Analysis for every batch.
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL; assay sensitivity 0.005 EU/mL.
Particulate — USP <788> Method 1
NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm); light obscuration.
Osmolality — USP <785>
Target: 280 - 320 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-RPMI-QPR1X compares
FluxMPS™ DCP-RPMI-QPR1X vs. conventional 0.22 µm–filtered RPMI formulations.
| Parameter | DCP-RPMI-QPR1X (FluxMPS™) | Conventional RPMI 1640 (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not applicable (0.22 µm filtered, no tier designation) | Not applicable (0.22 µm filtered, no tier designation) |
| RPMI 1640 without L-glutamine, pyruvate, and phenol red — independent metabolic/optical control | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm) | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation available | check_circle Yes | cancel No | cancel No |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-RPMI-QPR1X — RPMI 1640.
Supporting literature
Key publications supporting RPMI 1640 in immune cell culture and OoC applications.
- Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1001/jama.1967.03120080053007
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
