FluxMPS RPMI 1640 Medium w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Q: Does this medium require a CO2 incubator?

No. This formulation is bicarbonate-free and CO2-independent; supplement with 15-25 mM HEPES for pH buffering in open-well or atmospheric culture.

Q: What endotoxin level should I expect, and how is it verified?

FluxMPS DCP-RPMI-QBR1X is released to meet less than 0.05 EU/mL by LAL assay (USP ), controlled per manufacturing batch. For immune cells, endotoxin above the release specification can activate TLR4/NF-kB signaling, inducing cytokine release and altering lymphocyte activation state independently of the intended experimental variable.

verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640

Calcium Magnesium Glucose Sodium Pyruvate L-Glutamine Sodium Bicarbonate Phenol Red

FluxMPS™ DCP-RPMI-QBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 formulation manufactured without L-Glutamine, Sodium Bicarbonate, or Phenol Red, engineered for hematopoietic cells and related lymphocyte models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

Formulated without L-Glutamine, Sodium Bicarbonate or Phenol Red, and supplied with 2.0 g/L glucose and sodium pyruvate as an open-system, CO₂-independent RPMI 1640 base for immune cell assays
Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size
Endotoxin release specification < 0.05 EU/mL by LAL assay (USP <85>), controlled per manufacturing batch
Contains glutathione (reduced) for antioxidant support in redox-sensitive lymphocyte and hematopoietic cell models
CO₂-independent formulation; supplement with 15–25 mM HEPES for pH buffering in open-well or atmospheric culture
Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
Custom pH, nutrient, and salt modifications available on request — contact support@diagnocine.com

CAT. NO.

DCP-RPMI-QBR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

RPMI 1640 — 1X Liquid

Media familyRPMI 1640
Formulation[+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
AppearancePale yellow-colored, clear solution
Glucose2000 mg/L (2.0 g/L)
pH (USP <791>)7.4
Osmolality (USP <785>)230–270 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Storage2–8°C, away from light
Shelf Life12 months from date of manufacture, unopened

ISO 13485:2016 USP <85> <785> <788> RUO

Available pack sizes: 500 mL, 1000 mL.

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma, subvisible particulates, and endotoxin fragments that can activate TLR4 signaling and confound immune cell assays. FluxMPS™ is built to address these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate media for suspension immune cell OoC and flow cytometry.

science

Immune cell–optimized formulation

RPMI 1640 contains glutathione (antioxidant) and a balanced profile of vitamins and amino acids suited to lymphocyte and hematopoietic cell culture.

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Ultrapure-grade water

Type 1 water (18.2 MΩ·cm), controlled for trace metals and organic carbon (TOC) to minimize contaminant background in sensitive lymphocyte culture.

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Low endotoxin specification

Released to < 0.05 EU/mL, reducing the risk of LPS-driven T cell and NK cell activation artefacts in immune assays.

analytics

Rich nutrient profile

RPMI contributes specialized amino acid and vitamin content for demanding immune cell types, delivered at low particulate baseline for OoC use.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish.

1

0.1 µm Prefiltration I

Removes large aggregates, cell debris and protein aggregates; protects the first 0.04 µm cartridge.
2

0.04 µm Final filtration I

First 0.04 µm mycoplasma-retentive pass; retains sub-micron particulates and mycoplasma (0.2–0.3 µm) that pass a standard 0.22 µm filter.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter; aseptic fill & finish in ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size — mycoplasma-retentive polishing

Sterility: No growth after 14-day incubation (USP <71>). Mycoplasma is controlled by 0.04 µm mycoplasma-retentive filtration (not tested per lot unless specifically requested).

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS DCP-RPMI-QBR1X RPMI 1640 quadruple-stage filtration system, 0.1 micron x2 and 0.04 micron x2, for microfluidic and organ-on-a-chip applications, Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMI-QBR1X

Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMI-QBR1X — RPMI 1640 — delivers 0.04 µm filtered purity for hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion and robotic handling systems.

Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration reduces particulate load that can confound immune assays.

T cellsB cellsNK cellsPBMC

Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM would alter proliferation and signaling.

JurkatRajiK562HL-60

Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models where low particulate media is preferred.

Tumor-immune chipLymph node OoCMPS

Immunotherapy

CAR-T & TIL Expansion

A low endotoxin specification (<0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols where LPS-driven activation artefacts are a concern.

CAR-TTILTCR-T

Metabolomics

Immune Cell Metabolic Flux

Bicarbonate-free, phenol red–free base compatible with Agilent Seahorse XF T cell metabolic assays, glycolysis stress tests, and ¹³C isotope tracing of lymphocyte activation states.

Seahorse XF13C tracingGlycolysis

Live-Cell Imaging

Flow Cytometry & Confocal

Low particulate baseline supports PE-channel flow cytometry and immune cell confocal imaging; phenol red is excluded from this formulation to reduce dye-related optical interference.

Flow cytometryConfocalELISA

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate \| [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
Appearance	Pale yellow-colored, clear solution
Glucose	2000 mg/L (2.0 g/L)
pH USP <791>	7.4
Osmolality USP <785>	230–270 mOsm/kg H₂O
Total ingredients	38

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

RPMI 1640: 38 ingredients released per lot with CAS numbers for full raw-material traceability. This formulation contains glutathione (antioxidant) and a balanced profile of vitamins and amino acids suited to lymphocyte and hematopoietic cell culture.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate	13477-34-4	100.000
Magnesium sulfate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	400.000
Sodium chloride	7647-14-5	6000.000
Sodium phosphate dibasic anhydrous	7558-80-7	800.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	10.000
L-Arginine hydrochloride	1119-34-2	241.000
L-Asparagine	70-47-3	50.000
L-Aspartic acid	56-84-8	20.000
L-Cystine dihydrochloride	30925-07-6	65.200
L-Glutamic acid	56-86-0	20.000
L-Histidine hydrochloride monohydrate	5934-29-2	20.960
L-Hydroxyproline	51-35-4	20.000
L-Isoleucine	73-32-5	50.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	40.000
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	15.000
L-Proline	147-85-3	20.000
L-Serine	56-45-1	30.000
L-Threonine	72-19-5	20.000
L-Tryptophan	73-22-3	5.000
L-Tyrosine Disodium Salt	69847-45-6	28.830
L-Valine	72-18-4	20.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	3.000
D-Biotin	58-85-5	0.200
D-Ca-Pantothenate	137-08-6	0.250
Folic acid	59-30-3	1.000
Niacinamide	98-92-0	1.00
Pyridoxine hydrochloride	58-56-0	1.00
Riboflavin	83-88-5	0.200
Thiamine hydrochloride	67-03-8	1.000
Vitamin B12	68-19-9	0.005
i-Inositol	87-89-8	35.000
p-Amino benzoic acid (PABA)	150-13-0	1.000
OTHERS
D-Glucose	50-99-7	2000.000
Glutathione reduced	70-18-8	1.000
Sodium pyruvate	113-24-6	110.000

Custom formulation: Contact support@diagnocine.com for DCP-RPMI-QBR1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured by ISO 13485:2016–certified suppliers. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm, trace-metal and organic carbon (TOC) controlled.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration count: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Certificate of Analysis: Request for any DCP-RPMI-QBR1X lot at support@diagnocine.com.

Product Comparison

How DCP-RPMI-QBR1X compares

FluxMPS™ DCP-RPMI-QBR1X specifications compared against published supplier specifications.

Parameter	DCP-RPMI-QBR1X (FluxMPS™)	Comparison
Grade	Microfluidics Suitable	Not specified
Formulation	[+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate \| [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red	Not specified
Final filtration pore size	0.04 µm	Not specified
Number of filtration stages	4 (Quadruple-stage)	Not specified
Mycoplasma-retentive filtration	check_circle Yes (0.04 µm)	Not specified
Endotoxin (release specification)	< 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance	check_circle Yes (Method 1)	Not specified
Water quality	Type 1, 18.2 MΩ·cm	Not specified
Manufacturing QMS	ISO 13485:2016	Not specified
Microfluidic channel compatibility	check_circle Yes — engineered for microchannel use	Not specified
Custom formulation	Available on request	Not specified

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMI-QBR1X — RPMI 1640.

Is DCP-RPMI-QBR1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-RPMI-QBR1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering a low particulate baseline for microphysiological system (MPS), OoC, and LoC platforms. RPMI 1640 with mycoplasma-retentive filtration is suited to immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

Why are L-Glutamine, Sodium Bicarbonate, and Phenol Red all excluded, and how do I supplement for my cell type?expand_more

This base is bicarbonate-free and phenol red–free, giving a CO₂-independent, low-autofluorescence formulation compatible with HEPES buffering and Agilent Seahorse XF assays. L-Glutamine is left out so users can add it fresh at the concentration and stability profile (free amino acid or dipeptide) their protocol requires; glucose (2.0 g/L) and sodium pyruvate are already included as carbon sources.

Does this medium require a CO₂ incubator?expand_more

No. This formulation is bicarbonate-free and CO₂-independent; supplement with 15–25 mM HEPES for pH buffering in open-well or atmospheric culture.

Can DCP-RPMI-QBR1X be supplemented with serum, growth factors, or other components?expand_more

Yes. Add FBS (5–10%), serum-free supplements, growth factors, L-Glutamine, or antibiotics as required. When adding serum or protein-containing supplements after formulation, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — do not use a 0.04 µm filter, which retains IgM, VLDL, chylomicrons and much of the lipid/lipoprotein fraction of serum. Contact support@diagnocine.com for custom co-formulation.

What endotoxin level should I expect, and how is it verified?expand_more

FluxMPS™ DCP-RPMI-QBR1X is released to meet < 0.05 EU/mL by LAL assay (USP <85>), controlled per manufacturing batch. For immune cells, endotoxin above the release specification can activate TLR4/NF-κB signaling, inducing cytokine release and altering lymphocyte activation state independently of the intended experimental variable.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting RPMI 1640 in immune cell culture and OoC applications.

Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid