FluxMPS RPMI 1640 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Q: What makes the filtration process superior to standard 0.22 um filtered media?

FluxMPS uses four sequential filtration passes: 0.1 um prefiltration I, 0.04 um final filtration I, 0.1 um prefiltration II, and 0.04 um final filtration II (polish). Add L-glutamine (2 mM) or a stabilized glutamine dipeptide at the time of use, since free L-glutamine degrades in liquid storage. This configuration suits suspension lymphocyte assays run in atmospheric (non-CO2) environments requiring fresh nitrogen control.

Q: Does this medium require a CO2 incubator?

No. This formulation is CO2-independent; supplement with HEPES (15-25 mM) for pH buffering when culturing outside a CO2 incubator.

Q: What endotoxin level should I expect, and how is it verified?

FluxMPS DCP-RPMI-QB1X is released to meet less than 0.05 EU/mL by LAL assay (USP ), tested per manufacturing batch, with an assay sensitivity of 0.005 EU/mL. Bacterial endotoxin (LPS) is a well-characterized activator of TLR4/NF-kB signaling in immune cells; controlling endotoxin at this level reduces the risk of confounding cytokine release and activation-marker artefacts in lymphocyte and monocyte assays.

verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Bicarbonate — 1X Liquid

Phenol Red Calcium Magnesium Glucose (2.0 g/L) Sodium Pyruvate L-Glutamine Sodium Bicarbonate

FluxMPS™ DCP-RPMI-QB1X is a Microfluidics Suitable, ultra-filtered RPMI 1640 formulation engineered for hematopoietic cells, lymphocytes, and related immune cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L), [+] Sodium Pyruvate | [-] L-Glutamine, [-] Sodium Bicarbonate.

Glucose source retained at 2000 mg/L (2.0 g/L) — a complete carbon source despite the bicarbonate-free formulation
Glutathione (reduced), 1.0 mg/L — antioxidant support for redox-sensitive lymphocyte and hematopoietic cultures
Quadruple-stage filtration: 0.1 µm prefilter I → 0.04 µm final filter I → 0.1 µm prefilter II → 0.04 µm final filter II, reaching a 0.04 µm final cut-off
Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
Bicarbonate-free, glutamine-free formulation for CO₂-independent culture; supplement with HEPES (15–25 mM) for pH buffering and L-glutamine (2 mM, or a stabilized dipeptide) at time of use
Sodium pyruvate (110 mg/L) included as an alternative energy substrate for metabolically demanding immune cell types
Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
Customization available: pH, HEPES, glutamine, and salts adjustable on request — contact support@diagnocine.com

CAT. NO.

DCP-RPMI-QB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

RPMI 1640 — 1X Liquid, w/o L-Glutamine & Sodium Bicarbonate

Media familyRPMI 1640
Glucose2000 mg/L (2.0 g/L)
Sodium Pyruvate110 mg/L
L-GlutamineNone / Not added
pH (USP <791>)7.4
Osmolality (USP <785>)230–270 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
Storage2–8°C, protect from light
Shelf Life12 months from date of manufacture, unopened

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that activate TLR4 and confound immune cell assays. FluxMPS™ is built to address these failure modes at the filtration level.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate media suited to suspension immune cell OoC and flow cytometry workflows.

science

Immune cell–optimized formulation

RPMI 1640 contains reduced glutathione and a balanced amino acid and vitamin profile suited to lymphocyte and hematopoietic cell culture.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) with controlled trace-metal and total organic carbon (TOC) content, supporting reproducible lot-to-lot culture performance.

shield

Low endotoxin release specification

< 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch — relevant where LPS-driven TLR4/NF-κB activation can confound lymphocyte and monocyte assays.

analytics

Rich nutrient profile

RPMI 1640's amino acid and vitamin profile is delivered through a low-particulate filtration process suited to OoC and microfluidic use.

tune

Customization on demand

pH, HEPES, glutamine, and other component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish. Each 0.04 µm final filter is protected by its own dedicated 0.1 µm prefilter.

1

0.1 µm Prefiltration I

Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter would pass.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) environment.

Performance vs. conventional media

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm final pore size across 4 filtration passes

Sterility & mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration built into the process (mycoplasma diameter approximately 0.2–0.3 µm); this is a filtration control, not a per-lot mycoplasma assay result.

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS RPMI 1640 Medium w/o L-Glutamine, Sodium Bicarbonate (DCP-RPMI-QB1X) Quadruple-stage 0.1 micron x2 and 0.04 micron x2 filtration system for organ-on-a-chip, microfluidic, and immune cell culture applications - Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMI-QB1X

Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMI-QB1X — RPMI 1640 w/o L-Glutamine, Sodium Bicarbonate — delivers 0.04 µm filtered purity for hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion systems.

Total particulate exclusion: 0.01 µm filtration removes nanoparticulate aggregates
Valve & sensor protection: reduces micro-fouling risk in automated perfusion systems
Extended perfusion stability: consistent nutrient delivery over long-duration culture

Inquiry required: contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is a standard base for primary T cells, B cells, NK cells, and monocytes. 0.04 µm filtration reduces particulate-driven TLR activation that can confound immune assays.

T cellsB cellsNK cellsPBMC

Cancer Biology

Leukemia & Lymphoma Lines

RPMI 1640 supports Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM-based media can alter proliferation and signaling.

JurkatRajiK562HL-60

Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models where low-particulate media supports reproducible immune readouts.

Tumor-immune chipLymph node OoCMPS

Immunotherapy

CAR-T & TIL Expansion

A low endotoxin release specification (<0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols sensitive to LPS-driven activation artefacts.

CAR-TTILTCR-T

Metabolomics

Immune Cell Metabolic Flux

Defined RPMI base suited to ¹³C isotope tracing and glycolytic flux analysis of lymphocyte activation states via LC-MS/GC-MS workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.

¹³C tracingGlycolysis

Live-Cell Imaging

Flow Cytometry & Confocal

Ultra-low particulate media for flow cytometry and immune cell confocal imaging. A phenol red-free variant of this formulation is available on request for autofluorescence-sensitive PE-channel work.

Flow cytometryConfocalELISA

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L), [+] Sodium Pyruvate \| [-] L-Glutamine, [-] Sodium Bicarbonate
Appearance	Red-orange, clear solution
Glucose	2000 mg/L (2.0 g/L)
pH USP <791>	7.4
Osmolality USP <785>	230–270 mOsm/kg H₂O
Total ingredients	39

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

39 ingredients, individually traceable by CAS number, organized into Inorganic Salts, Amino Acids, and Vitamins & Others tabs below.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate	13477-34-4	100.000
Magnesium sulfate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	400.000
Sodium chloride	7647-14-5	6000.000
Sodium phosphate dibasic anhydrous	7558-79-4	800.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	10.000
L-Arginine hydrochloride	1119-34-2	241.000
L-Asparagine	70-47-3	50.000
L-Aspartic acid	56-84-8	20.000
L-Cystine dihydrochloride	30925-07-6	65.200
L-Glutamic acid	56-86-0	20.000
L-Histidine hydrochloride monohydrate	5934-29-2	20.960
L-Hydroxyproline	51-35-4	20.000
L-Isoleucine	73-32-5	50.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	40.000
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	15.000
L-Proline	147-85-3	20.000
L-Serine	56-45-1	30.000
L-Threonine	72-19-5	20.000
L-Tryptophan	73-22-3	5.000
L-Tyrosine Disodium Salt	69847-45-6	28.830
L-Valine	72-18-4	20.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	3.000
D-Biotin	58-85-5	0.200
D-Ca-Pantothenate	137-08-6	0.250
Folic acid	59-30-3	1.000
Niacinamide	98-92-0	1.00
Pyridoxine hydrochloride	58-56-0	1.00
Riboflavin	83-88-5	0.200
Thiamine hydrochloride	67-03-8	1.000
Vitamin B12	68-19-9	0.005
i-Inositol	87-89-8	35.000
p-Amino benzoic acid (PABA)	150-13-0	1.000
OTHERS
D-Glucose	50-99-7	2000.000
Glutathione reduced	70-18-8	1.000
Phenol red sodium salt	34487-61-1	5.300
Sodium pyruvate	113-24-6	110.000

Custom formulation: Contact support@diagnocine.com for DCP-RPMI-QB1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine's Totowa, NJ, USA facility.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm with controlled trace-metal and TOC content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; a Certificate of Analysis is issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per manufacturing batch; assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance, and clarity
Sterility

A Certificate of Analysis is available on request at support@diagnocine.com.

Product Comparison

How DCP-RPMI-QB1X compares

FluxMPS™ DCP-RPMI-QB1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter	DCP-RPMI-QB1X (FluxMPS™)	Conventional RPMI 1640 (0.22 µm filtered)	Standard alternative (0.22 µm filtered)
Grade	Microfluidics Suitable	Standard grade	Standard grade
RPMI 1640 without L-Glutamine and Sodium Bicarbonate — CO₂-independent formulation	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma barrier filtration	check_circle 0.1 µm retentive filtration	cancel No	cancel No
Endotoxin (release specification)	< 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate compliance	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatibility	check_circle Microfluidics Suitable	cancel Risk of clogging	cancel Risk of clogging
Custom formulation	check_circle Yes, on request	cancel No	cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMI-QB1X — RPMI 1640 w/o L-Glutamine, Sodium Bicarbonate.

Is DCP-RPMI-QB1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-RPMI-QB1X is a Microfluidics Suitable RPMI 1640 formulation processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low-particulate media for OoC and microphysiological system (MPS) platforms. It is suited to immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

FluxMPS™ uses four sequential filtration passes: 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish).

Why are L-Glutamine and Sodium Bicarbonate both excluded from DCP-RPMI-QB1X, and how do I supplement?expand_more

This formulation is bicarbonate-free and glutamine-free, making it CO₂-independent when supplemented with HEPES (15–25 mM) for pH buffering. Add L-glutamine (2 mM) or a stabilized glutamine dipeptide at the time of use, since free L-glutamine degrades in liquid storage. This configuration suits suspension lymphocyte assays run in atmospheric (non-CO₂) environments requiring fresh nitrogen control.

Does this medium require a CO₂ incubator?expand_more

No. This formulation is CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering when culturing outside a CO₂ incubator.

Can DCP-RPMI-QB1X be supplemented with serum, growth factors, or other components?expand_more

Yes. FBS (5–10%), serum-free supplements, growth factors, or antibiotics may be added post-thaw. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm membrane for serum, as it retains immunoglobulins, lipoproteins, and growth factors. Contact support@diagnocine.com for custom co-formulation.

What endotoxin level should I expect, and how is it verified?expand_more

FluxMPS™ DCP-RPMI-QB1X is released to meet < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch, with an assay sensitivity of 0.005 EU/mL. Bacterial endotoxin (LPS) is a well-characterized activator of TLR4/NF-κB signaling in immune cells; controlling endotoxin at this level reduces the risk of confounding cytokine release and activation-marker artefacts in lymphocyte and monocyte assays.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting RPMI 1640 in immune cell culture and OoC applications.

Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid