FluxMPS™ RPMI 1640 Medium w/o Folic Acid: 1X Liquid
FluxMPS™ RPMI 1640 Medium w/o Folic Acid
FluxMPS™ DCP-RPMI-F1X is a Microfluidics Suitable, quadruple-stage ultra-filtered RPMI 1640 formulation engineered for lymphocytes, hematopoietic cell lines, and related immune cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] 2 g/L Glucose, [+] Sodium Pyruvate | [-] Folic Acid.
- Carbon source: D-Glucose 2000 mg/L (2.0 g/L) — standard RPMI energy substrate for lymphocyte and hematopoietic culture
- Folic acid intentionally excluded — supports methotrexate (MTX) resistance studies, DHFR inhibitor screening, and folate-receptor pathway research
- Glutathione (reduced), 1.000 mg/L, included — antioxidant support for redox-sensitive immune cell types
- Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm final polish, reaching a 0.04 µm final cut-off
- Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85>), controlled per manufacturing batch
- Sodium bicarbonate–buffered (2.0 g/L) — formulated for a standard 5% CO₂ incubator atmosphere
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- pH, nutrient concentrations, HEPES buffering, and component modifications available on request
- Media familyRPMI 1640
- Glucose2000 mg/L (2.0 g/L)
- Folic AcidNot added
- AppearanceRed-colored, clear solution (phenol red present)
- pH (USP <791>)7.4
- Osmolality (USP <785>)280–320 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- FiltrationQuadruple-stage: 0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Available pack sizes: 500 mL, 1000 mL.
Engineered where standard media fails
Conventional 0.22 µm–filtered RPMI passes mycoplasma-scale organisms, subvisible particulates, and endotoxin fragments capable of activating TLR4 signaling — all of which can confound sensitive immune cell assays. FluxMPS™ is built to reduce these failure modes.
Microchannel-safe purity
0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate testing supports ultra-low-particulate media delivery for suspension immune cell OoC and downstream flow cytometry.
Immune cell–optimized formulation
RPMI 1640 contains reduced glutathione and a balanced amino acid/vitamin profile suited to lymphocyte and hematopoietic cell culture.
Ultrapure-grade water
Type 1 water, 18.2 MΩ·cm, formulated with tight trace-metal and organic-carbon (TOC) control.
Held to a strict endotoxin specification
< 0.05 EU/mL release specification — relevant where LPS-driven TLR4/NF-κB signaling can alter T-cell and NK-cell activation readouts.
Rich nutrient profile
RPMI's specialized nutrient set for demanding immune cell types, delivered as an ultra-low-particulate liquid for OoC use.
Customization on demand
pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration passes, alternating a 0.1 µm prefilter with a 0.04 µm final filter, reach a final 0.04 µm cut-off. Each 0.04 µm final filter has its own dedicated 0.1 µm prefilter — the train is two protected pairs run in series, not a single descending cascade.
-
1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; a mycoplasma-retentive grade pore size (mycoplasma organisms are typically 0.2–0.3 µm), retaining sub-micron particulates a 0.22 µm filter would pass.
-
3
0.1 µm Prefiltration II
A second, dedicated prefilter protecting the second 0.04 µm cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish in validated ISO Class 5 (Class 100) laminar-flow workstations.
Performance vs. conventional media
© Diagnocine® — DCP-RPMI-F1X
Immune cell OoC and hematopoietic applications
FluxMPS™ DCP-RPMI-F1X — RPMI 1640 w/o Folic Acid — delivers 0.04 µm filtered, Microfluidics Suitable media for hematopoietic cells and related OoC applications.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic perfusion systems.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
T Cell & Lymphocyte Culture
RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration reduces particulate-driven TLR4 activation that can confound immune assays.
Leukemia & Lymphoma Lines
RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and hematopoietic cancer lines generally, where DMEM would alter proliferation and signaling.
Immune Cell OoC
0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models where ultra-low-particulate media is preferred.
CAR-T & TIL Expansion
A <0.05 EU/mL endotoxin release specification is relevant to CAR-T manufacturing and TIL expansion protocols, where LPS-driven T-cell activation artefacts are a known confound.
Immune Cell Metabolic Flux
Defined RPMI base for lymphocyte metabolic profiling via ¹³C isotope tracing and NMR metabolomics. Note: not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium.
Flow Cytometry & Confocal
Ultra-low particulate baseline benefits flow cytometry and immune cell confocal imaging. This formulation contains phenol red; a phenol red–free variant is available on request for fluorescence-sensitive assays.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] 2 g/L Glucose, [+] Sodium Pyruvate | [-] Folic Acid |
| Appearance | Red-colored, clear solution |
| Glucose | 2000 mg/L (2.0 g/L) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 280–320 mOsm/kg H₂O |
| Total ingredients | 40 (3 categories: Inorganic Salts, Amino Acids, Vitamins & Others) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ required (based on 2.0 g/L sodium bicarbonate buffering) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
RPMI 1640 w/o Folic Acid: 40 ingredients per lot, with CAS numbers for raw-material traceability. Contains reduced glutathione and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium nitrate tetrahydrate | 13477-34-4 | 100.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 48.840 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2000.000 |
| Sodium chloride | 7647-14-5 | 6000.00 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 800.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 10.000 |
| L-Arginine hydrochloride | 1119-34-2 | 241.000 |
| L-Asparagine | 70-47-3 | 50.000 |
| L-Aspartic acid | 56-84-8 | 20.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 65.200 |
| L-Glutamic acid | 56-86-0 | 20.000 |
| L-Glutamine | 56-85-9 | 300.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Hydroxyproline | 51-35-4 | 20.000 |
| L-Isoleucine | 73-32-5 | 50.000 |
| L-Leucine | 61-90-5 | 50.000 |
| L-Lysine hydrochloride | 657-27-2 | 40.000 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 15.000 |
| L-Proline | 147-85-3 | 20.000 |
| L-Serine | 56-45-1 | 30.000 |
| L-Threonine | 72-19-5 | 20.000 |
| L-Tryptophan | 73-22-3 | 5.000 |
| L-Tyrosine Disodium Salt | 69847-45-6 | 28.830 |
| L-Valine | 72-18-4 | 20.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 3.000 |
| D-Biotin | 58-85-5 | 0.200 |
| D-Ca-Pantothenate | 137-08-6 | 0.250 |
| Niacinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| Vitamin B12 | 68-19-9 | 0.005 |
| p-Amino benzoic acid (PABA) | 150-13-0 | 1.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 35.000 |
| D-Glucose | 50-99-7 | 2000.000 |
| Glutathione reduced | 70-18-8 | 1.000 |
| Phenol red sodium salt | 34487-61-1 | 5.300 |
| Sodium Pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.
Quadruple-Stage Filtration
0.1 µm ×2 + 0.04 µm ×2 filtration train applied to every batch prior to fill.
ISO Class 5 Fill & Finish
Final 0.04 µm polish and aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending. Certificate of Analysis available for every lot.
Endotoxin — USP <85> BET
LAL assay; batch release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: 280–320 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-RPMI-F1X compares
FluxMPS™ DCP-RPMI-F1X vs. conventional 0.22 µm–filtered RPMI formulations.
| Parameter | DCP-RPMI-F1X (FluxMPS™) | Conventional RPMI 1640 (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm cut-off) | Not applicable (0.22 µm) | Not applicable (0.22 µm) |
| RPMI 1640 without Folic Acid — for folate pathway research, MTX resistance, and DHFR inhibitor studies in immune cells | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm stage) | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Yes | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation available | check_circle Yes | cancel No | cancel No |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-RPMI-F1X — RPMI 1640 w/o Folic Acid.
Supporting literature
Key publications supporting RPMI 1640 in immune cell culture and organ-on-a-chip applications.
- Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1001/jama.1967.03120080053007
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Junkin M, et al. High-content quantification of single-cell immune dynamics. Cell Rep. 2016;15:411–422. doi:10.1016/j.celrep.2016.03.033
- Parlato S, et al. 3D microfluidic model for evaluating immunotherapy efficacy. Sci Rep. 2017;7:1093. doi:10.1038/s41598-017-01013-x
- Rankin GO, et al. Bacterial endotoxin (LPS) and immune cell activation via TLR4. Toxicol Lett. 2010;199:307–314. doi:10.1016/j.toxlet.2010.09.017
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
