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FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-RPMIH-PBR1X
$44.00
DCP-RPMIH-PBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 + 25mM HEPES

Contains L-Glutamine Contains 25mM HEPES Contains Calcium Contains Magnesium Contains 2.0 g/L Glucose Without Sodium Bicarbonate Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-RPMIH-PBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 + 25 mM HEPES formulation engineered for hematopoietic cells, lymphocytes, and immune cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The quadruple-stage train reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) buffers pH independently of a CO2 incubator, and the formulation omits sodium pyruvate, sodium bicarbonate, and phenol red for a defined, atmosphere-stable base. Formulation: [+] L-Glutamine, [+] 25 mM HEPES, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose | [-] Sodium Bicarbonate, [-] Phenol Red, [-] Sodium Pyruvate.

  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off, five times finer than standard 0.22 µm sterile filtration
  • 25 mM HEPES (pKa 7.3 at 37°C) provides CO2-independent pH buffering for open-bench handling and flow cytometry workflows
  • Formulated without sodium pyruvate, sodium bicarbonate, or phenol red for a defined, atmosphere-stable, low-background base
  • 2.0 g/L (2000 mg/L) D-glucose and 300 mg/L L-glutamine support hematopoietic and lymphocyte proliferation
  • Reduced glutathione (1.0 mg/L) included for redox support in immune cell culture
  • Endotoxin release specification < 0.05 EU/mL by LAL assay (USP <85>)
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom pH, HEPES concentration, and nutrient modifications available on request
CAT. NO.
DCP-RPMIH-PBR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 + 25mM HEPES — 1X Liquid
  • Media familyRPMI 1640 + 25mM HEPES
  • Glucose2000 mg/L (2.0 g/L)
  • HEPES25 mM (pKa 7.3 at 37°C)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that can activate TLR4 signaling and confound immune cell assays.[1,2] FluxMPS™ is built to address these gaps.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 particulate compliance, supporting suspension immune cell OoC and flow cytometry workflows sensitive to particulate interference.

science

Immune cell–optimized formulation

RPMI 1640 contributes reduced glutathione (antioxidant) and a balanced amino acid and vitamin profile suited to lymphocyte and hematopoietic cell culture.

water_drop

Ultrapure-grade water

Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon (TOC) control during manufacture.

shield

Low endotoxin release specification

< 0.05 EU/mL by LAL assay (USP <85>) helps reduce the risk of LPS-driven TLR4 activation artifacts in T cell and NK cell assays.

visibility

Low background for imaging

0.04 µm final filtration yields an ultra-low particulate baseline supporting confocal microscopy and optical biosensor readouts; no phenol red is added to this formulation.

tune

Customization on demand

pH, HEPES concentration, nutrient levels, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reach a final 0.04 µm polish. Each 0.04 µm final filter is protected by its own 0.1 µm prefilter, giving full redundancy across the train.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter would not.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

The quadruple-stage train reaches a 0.04 µm final pore size — five times finer than the 0.22 µm membranes used for conventional sterile filtration — with two independent 0.1 µm/0.04 µm filter pairs run in series.

5×
Finer pore size than conventional 0.22 µm filtration
0.04
µm final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control (0.2–0.3 µm organisms) is achieved through 0.1 µm mycoplasma-retentive filtration; this is a filtration-based control, not a per-lot mycoplasma test.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMIH-PBR1X RPMI 1640 25mM HEPES quadruple-stage filtration system diagram, 0.1 micron x2 plus 0.04 micron x2, for organ-on-a-chip and microfluidic cell culture applications, Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIH-PBR1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-PBR1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered purity for hematopoietic and lymphocyte-based OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available on request for automated perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is a standard base for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration reduces particulate load relevant to TLR4 activation in immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports hematopoietic cancer cell lines including Jurkat, Raji, K562, and HL-60, where DMEM-type media can alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models built on microphysiological system (MPS) platforms.[3,4]

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Low endotoxin release specification (<0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols sensitive to LPS-driven activation artifacts.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Bicarbonate-free, HEPES-buffered base compatible with Agilent Seahorse XF T cell metabolic assays, glycolysis stress tests, and 13C isotope tracing of lymphocyte activation states.

Seahorse XF13C tracingGlycolysis
Live-Cell Imaging

Flow Cytometry & Confocal

Phenol red-free formulation (no phenol red added) reduces background fluorescence for PE-channel flow cytometry and confocal imaging of immune cells.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix below. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose | [-] Sodium Bicarbonate, [-] Phenol Red, [-] Sodium Pyruvate
Appearance Pale yellow-colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H2O
Total ingredients 39 components (Inorganic Salts, Amino Acids, Vitamins, Others)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (release specification; see §Manufacturing)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — 25 mM HEPES maintains pH 7.2–7.4 at 37°C
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)

Available pack sizes: 500 mL, 1000 mL

Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 39 ingredients verified per lot with CAS numbers for raw-material traceability. This formulation includes reduced glutathione (antioxidant) and a balanced profile of amino acids and vitamins suited to lymphocyte and hematopoietic cell culture.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
HEPES 7365-45-9 5958.000
Glutathione reduced 70-18-8 1.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-PBR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water for trace-metal and organic-carbon (TOC) control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay, assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIH-PBR1X compares

FluxMPS™ DCP-RPMIH-PBR1X vs. conventional 0.22 µm-filtered RPMI formulations and published supplier endotoxin specifications.

Parameter DCP-RPMIH-PBR1X (FluxMPS™) Conventional RPMI 1640 + HEPES (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
Formulation trait: HEPES-buffered, no pyruvate/bicarbonate/phenol red check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration (0.1 µm) check_circle Yes cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate testing check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Higher clogging risk cancel Higher clogging risk
Custom formulation available check_circle Yes cancel Typically no cancel Typically no

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-PBR1X — RPMI 1640 + 25mM HEPES.

Yes. DCP-RPMIH-PBR1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size and is Microfluidics Suitable. RPMI 1640 with HEPES buffering and sub-mycoplasma filtration is well suited to immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.
FluxMPS™ uses four sequential filters in two prefilter/final-filter pairs: 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish). The 0.04 µm final cut-off is five times finer than the 0.22 µm membranes used in conventional sterile filtration.
This formulation is a HEPES-buffered, atmosphere-stable RPMI base for workflows where sodium bicarbonate buffering, phenol red pH indication, or an additional pyruvate carbon source are undesirable — for example extended bench-top handling, flow cytometry, and imaging protocols sensitive to background fluorescence. Glucose (2.0 g/L) and L-glutamine remain as the primary carbon and nitrogen sources. If your protocol calls for sodium pyruvate or sodium bicarbonate-buffered incubation, both can be added at time of use; contact support@diagnocine.com for guidance specific to your cell model.
No. This formulation contains no sodium bicarbonate and is CO2-independent — 25 mM HEPES alone maintains pH 7.2–7.4 at 37°C, making it suitable for open-bench handling and ambient-air workflows.
Yes. Fetal bovine serum (typically 5–10%), serum-free supplements, growth factors, or antibiotics can be added. When adding serum or protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane rather than a 0.04 µm membrane, which will retain serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
DCP-RPMIH-PBR1X is manufactured to a release specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification. For immune cell assays, elevated endotoxin can activate TLR4/NF-κB signaling and alter lymphocyte activation state independently of experimental conditions.
Yes. Each lot's CoA covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration control, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 + HEPES formulations in immune cell culture and organ-on-a-chip applications.

  1. Moore GE, Gerner RE, Franklin HA. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1001/jama.1967.03120080053007
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  6. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
  7. Ando Y, et al. Peripheral blood mononuclear cell (PBMC) culture and analysis in microfluidic immune assay platforms. Lab Chip. 2017;17:1495–1508. doi:10.1039/c6lc01590g
  8. Ronaldson-Bouchard K, Vunjak-Novakovic G. Organs-on-a-chip: a fast track for engineered human tissues in drug development. Cell Stem Cell. 2018;22:310–324. doi:10.1016/j.stem.2018.02.011

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