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FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-RPMIH-PB1X
$44.00
DCP-RPMIH-PB1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 + 25mM HEPES

Contains L-Glutamine Contains Phenol Red Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains Glucose (2.0 g/L) Without Sodium Bicarbonate Without Sodium Pyruvate

FluxMPS™ DCP-RPMIH-PB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 + 25mM HEPES formulation engineered for hematopoietic cells and lymphocyte models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The train reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides CO₂-independent pH buffering. Formulation: [+] L-Glutamine, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) | [-] Sodium Bicarbonate, [-] Sodium Pyruvate.

  • Glucose source: 2000 mg/L (2.0 g/L) — standard carbon source for lymphocyte and hematopoietic cultures
  • 25 mM HEPES (pKa 7.3 at 37°C) provides CO₂-independent pH buffering for open-air handling and flow cytometry workflows
  • L-Glutamine included at 300 mg/L; formulated without sodium pyruvate and without sodium bicarbonate
  • Glutathione (reduced), 1.0 mg/L — antioxidant support for redox-sensitive lymphocyte and hematopoietic lineages
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Formulated with Type 1 ultrapure water (18.2 MΩ·cm)
CAT. NO.
DCP-RPMIH-PB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 + 25mM HEPES — 1X Liquid
  • Media familyRPMI 1640 + 25mM HEPES
  • Glucose2000 mg/L (2.0 g/L)
  • HEPES25 mM, pKa 7.3 at 37°C
  • Formulation[+] L-Glutamine, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) | [-] Sodium Bicarbonate, [-] Sodium Pyruvate
  • AppearanceOrange-red, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO

Pack sizes: 500 mL, 1000 mL. Pricing available through your Diagnocine account or by contacting support@diagnocine.com.

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma, subvisible particulates, and endotoxin fragments that can activate TLR4 signaling and confound immune cell assays. FluxMPS™ is built to address these failure modes at the filtration and formulation level.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Ultra-low particulate media for suspension immune cell OoC and flow cytometry.

science

Immune cell–optimized formulation

RPMI 1640 contains reduced glutathione (antioxidant) and a balanced amino acid and vitamin profile suited to lymphocyte and hematopoietic cell culture.

water_drop

Ultrapure-grade water

Formulated with Type 1 water (18.2 MΩ·cm) to minimize trace-metal and ionic background that could otherwise influence lymphocyte signaling assays.

shield

Low endotoxin release specification

< 0.05 EU/mL — formulated to help reduce the risk of LPS-driven TLR4 activation artefacts in T cell and NK cell assays.

water_drop

HEPES pH stability

HEPES (25 mM) helps limit pH rise during open-air handling, flow cytometry preparation, and atmospheric incubation.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two prefilter + final-filter pairs — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Large particulate, cell debris, and protein aggregate removal; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge; the 0.1 µm pore size is the mycoplasma-retentive grade.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 conditions.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

0.04
µm final pore size
4
Sequential filtration passes per lot
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS RPMI 1640 with 25mM HEPES DCP-RPMIH-PB1X Quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 for organ-on-a-chip and microfluidic cell culture applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIH-PB1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-PB1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered, Microfluidics Suitable media for hematopoietic and lymphocyte OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) MPS Grade ultra-filtered variant available on request for automated perfusion and robotic handling systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is a standard base for primary T cells, B cells, NK cells, and monocytes. 0.04 µm filtration is intended to reduce particulate exposure in sensitive immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM-based media may alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models designed around microfluidic channel geometries.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Low endotoxin release specification (<0.05 EU/mL) is intended to help reduce risk of LPS-driven T cell activation artefacts during CAR-T manufacturing and TIL expansion protocols.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Defined RPMI base suited to ¹³C isotope tracing and NMR metabolomics of lymphocyte activation states. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomicsGlycolysis
Live-Cell Imaging

Flow Cytometry & Confocal

Ultra-low particulate media suited to flow cytometry and confocal imaging of lymphocytes and hematopoietic lineages. Phenol red–free variants are available on request for applications requiring minimal background fluorescence.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L) | [-] Sodium Bicarbonate, [-] Sodium Pyruvate
Appearance Orange-red, clear solution
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H₂O
Total ingredients 40 components across 3 categories
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent — 25 mM HEPES alone maintains pH 7.2–7.4 at 37°C
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Grade Microfluidics Suitable (0.04 µm final cut-off)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 40 ingredients released per lot with CAS numbers for raw-material traceability. RPMI 1640 contains reduced glutathione (antioxidant) and a balanced profile of vitamins and amino acids suited to lymphocyte and hematopoietic cell culture.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 5.300
Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-PB1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016–certified quality management system. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water, controlled for trace-metal and organic-carbon content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot QC release — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay, assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H₂O.

Documentation & CoA

Full Certificate of Analysis with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIH-PB1X compares

FluxMPS™ DCP-RPMIH-PB1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMIH-PB1X (FluxMPS™) Conventional RPMI 1640 + HEPES (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
RPMI 1640 + HEPES-only, CO₂-independent; without pyruvate, without sodium bicarbonate check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration (0.1 µm) check_circle Yes cancel No cancel No
HEPES (25 mM) check_circle Yes cancel Usually no cancel No
Endotoxin specification < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-PB1X — RPMI 1640 + 25mM HEPES.

Yes. DCP-RPMIH-PB1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, a 0.04 µm cut-off Diagnocine classifies as Microfluidics Suitable. RPMI 1640 with this filtration purity is suited to immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.
FluxMPS™ uses four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. The 0.1 µm stages provide mycoplasma-retentive filtration.
This formulation is HEPES-buffered and CO₂-independent; L-Glutamine (300 mg/L) is included. If your protocol requires sodium pyruvate as a secondary carbon source, add it fresh (typically 1 mM) at time of use. Sodium bicarbonate is intentionally omitted so pH is controlled entirely by the 25 mM HEPES buffer rather than a CO₂-bicarbonate system.
No. This formulation is CO₂-independent — 25 mM HEPES alone maintains pH 7.2–7.4 at 37°C, making it suitable for open-bench handling, flow cytometry preparation, and ambient-air workflows.
Yes. FBS (5–10%), serum-free supplements, growth factors, or antibiotics can be added as required. If filtering a serum- or protein-containing addition, use a 0.2 µm low-protein-binding PES or PVDF filter — a 0.04 µm filter will strip serum proteins and lipoproteins and is not recommended for supplement filtration. Contact support@diagnocine.com for custom co-formulation.
DCP-RPMIH-PB1X is manufactured to a release specification of < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch rather than per unit. Endotoxin above typical thresholds can activate TLR4/NF-κB signaling, inducing cytokine release and altering lymphocyte activation state independently of experimental conditions, which is why immune cell assays are particularly sensitive to this specification.
Yes. A full CoA is available per lot and covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 + 25mM HEPES in immune cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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