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FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-RPMIH-PR1X
$44.00
DCP-RPMIH-PR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 + 25mM HEPES

Contains L-Glutamine Contains Sodium Bicarbonate Contains 25 mM HEPES Contains Calcium Contains Magnesium Contains 2 g/L Glucose Without Sodium Pyruvate Without Phenol Red

FluxMPS™ DCP-RPMIH-PR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 + 25mM HEPES formulation engineered for hematopoietic cells, lymphocytes and related immune cell models on microfluidic, organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The 0.04 µm final cut-off is five times finer than the 0.22 µm membranes used in conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) supplements the native 23.8 mM sodium bicarbonate buffer for stable pH during bench-top handling. Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25 mM HEPES, [+] Calcium, [+] Magnesium, [+] 2 g/L Glucose | [-] Sodium Pyruvate, [-] Phenol Red.

  • Glucose source: 2000 mg/L (2.0 g/L) D-Glucose, standard RPMI carbon source
  • 25 mM HEPES (pKa 7.3 at 37°C) supplements the 23.8 mM native sodium bicarbonate buffer for pH stability during open-bench handling
  • Glutathione (reduced), 1.0 mg/L — included for redox support in lymphocyte and hematopoietic cell culture
  • Phenol red–free and sodium pyruvate–free — formulated for autofluorescence-sensitive imaging and fresh pyruvate supplementation at time of use
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • 40 formulation components released per lot with CAS-level raw material traceability
CAT. NO.
DCP-RPMIH-PR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 + 25mM HEPES — 1X Liquid
  • Glucose2000 mg/L (2.0 g/L)
  • HEPES25 mM, pKa 7.3 at 37°C
  • Sodium PyruvateNot added
  • Phenol RedNot added
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)280-320 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized organisms, subvisible particulates and endotoxin fragments that can activate TLR4 signaling and confound immune cell assays. FluxMPS™ is built to address these failure modes at the filtration level.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance, supporting suspension immune cell OoC and flow cytometry workflows.

science

Immune cell–optimized formulation

RPMI 1640 contains reduced glutathione and a balanced amino acid and vitamin profile suited to lymphocyte and hematopoietic cell culture.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) processing controls trace-metal and organic carbon (TOC) contamination during manufacture.

shield

Batch-controlled endotoxin

< 0.05 EU/mL release specification per batch, reducing the risk of LPS-driven TLR4 activation artefacts in immune cell assays.

water_drop

HEPES pH stability

25 mM HEPES supplements native bicarbonate buffering, reducing pH drift during open-bench handling and flow cytometry preparation.

tune

Customization on demand

pH, nutrient concentrations, HEPES and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter-plus-final-filter pairs, reaching a final 0.04 µm polish under aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill and finish.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

5×
Finer final pore size vs. standard 0.22 µm filtration (0.22 ÷ 0.04)
0.04
µm final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma organisms (0.2–0.3 µm) are controlled through 0.1 µm mycoplasma-retentive filtration at each pass; the medium is not tested per lot against USP <63>.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS RPMI 1640 with 25mM HEPES DCP-RPMIH-PR1X Quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 for organ-on-a-chip and microfluidic cell culture applications Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) used to manufacture DCP-RPMIH-PR1X.
© Diagnocine® — DCP-RPMIH-PR1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-PR1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered, Microfluidics Suitable purity for hematopoietic cells, lymphocytes and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant of this formulation — ultra nano-filtered through an additional 0.02 µm and 0.01 µm stage — is available on request for automated bioreactors and robotic perfusion systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base for primary T cells, B cells, NK cells and monocytes. The 0.04 µm filtration reduces particulate load that can confound immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60 and other hematopoietic cancer lines where DMEM would alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC and lymph node-on-chip models where particulate load must be minimized.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

A <0.05 EU/mL endotoxin release specification helps reduce the risk of LPS-driven T cell activation artefacts in CAR-T manufacturing and TIL expansion protocols.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

A defined RPMI base with controlled glucose and glutamine input supports isotope tracing (¹³C, ¹&sup5;N) and metabolic flux studies of lymphocyte activation. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingMetabolic flux
Live-Cell Imaging

Flow Cytometry & Confocal

Ultra-low particulate baseline supports confocal imaging and biosensor integration; the phenol red-free formulation reduces spectral interference in the PE and FITC detection channels.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] 25 mM HEPES, [+] Calcium, [+] Magnesium, [+] 2 g/L Glucose | [-] Sodium Pyruvate, [-] Phenol Red
Appearance Colorless to pale yellow, clear solution
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 280-320 mOsm/kg H2O
Total ingredients 40 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 recommended (23.8 mM native sodium bicarbonate calibrated for 5% CO2; 25 mM HEPES provides supplemental buffering during bench-top handling)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 40 ingredients verified per lot with CAS numbers for full raw-material traceability. RPMI 1640 contains reduced glutathione and a balanced profile of vitamins and amino acids suited to lymphocyte and hematopoietic cell culture.

Inorganic Salts
Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2000.000
Sodium chloride 7647-14-5 6000.00
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Amino Acids
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Vitamins and Others
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.00
i-Inositol 87-89-8 35.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-PR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine, Totowa, NJ, USA.

filter_alt

Quadruple-Stage Filtration

0.1 µm ×2 + 0.04 µm ×2 filtration train reaching a 0.04 µm final cut-off before aseptic fill.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water; trace-metal and total organic carbon (TOC) controlled during manufacture.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

≤25/mL (≥10 µm), ≤3/mL (≥25 µm), by light obscuration.

Osmolality — USP <785>

Target: 280-320 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-RPMIH-PR1X lot at support@diagnocine.com.
Product Comparison

How DCP-RPMIH-PR1X compares

FluxMPS™ DCP-RPMIH-PR1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMIH-PR1X (FluxMPS™) Conventional RPMI 1640 + 25mM HEPES (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
RPMI 1640 + 25mM HEPES — no Pyruvate, no Phenol Red check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stages) cancel No cancel No
HEPES (25 mM) check_circle Yes cancel Usually no cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes (0.04 µm filtered) cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-PR1X — RPMI 1640 + 25mM HEPES.

Yes. DCP-RPMIH-PR1X is processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, and is Microfluidics Suitable for use in microfluidic, organ-on-a-chip and microphysiological system (MPS) platforms. RPMI 1640 with reduced particulate load is well suited to immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.
FluxMPS™ runs four sequential filtration passes — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional sterile filtration.
Phenol red is excluded to reduce spectral interference in fluorescence and flow cytometry channels. Sodium pyruvate is excluded so it can be added fresh at the time of use if your protocol requires it (typically 1 mM final concentration); many lymphocyte and hematopoietic protocols do not require added pyruvate. The formulation retains 25 mM HEPES together with native sodium bicarbonate buffering.
5% CO2 is recommended. The formulation's 23.8 mM native sodium bicarbonate is calibrated for standard 5% CO2 incubation; the additional 25 mM HEPES provides supplemental buffering during open-bench handling and imaging outside the incubator.
Yes. FBS (5-10%), serum-free supplements, growth factors, or antibiotics can be added as required. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane; do not use a 0.04 µm membrane for serum, as it retains IgM, lipoproteins and other serum components. Contact support@diagnocine.com for custom co-formulation.
DCP-RPMIH-PR1X is released against a batch specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch, and every batch is tested before release. A Certificate of Analysis is available on request.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 + 25mM HEPES in immune cell culture and OoC applications.

  1. Moore GE, Gerner RE, Franklin HA. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1001/jama.1967.03120080053007
  2. Huh D, Matthews BD, Mammoto A, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, Ingram M, Marquez S, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Boussommier-Calleja A, Li R, Chen MB, et al. Microfluidics: A new tool for modeling cancer-immune interactions. Trends Cancer. 2016;2:6–19. doi:10.1016/j.trecan.2015.12.003
  6. Ando Y, Siegler EL, Ta HP, et al. Evaluating CAR-T cell therapy in a hypoxic 3D tumor model. Adv Healthc Mater. 2019;8:e1900001. doi:10.1002/adhm.201900001
  7. Parlato S, De Ninno A, Molfetta R, et al. 3D Microfluidic model for evaluating immunotherapy efficacy. Sci Rep. 2017;7:1093. doi:10.1038/s41598-017-01013-x
  8. Rankin SM, Weninger W, Bunse M, et al. Lymph node-on-a-chip models for immune assessment. Lab Chip. 2021;21:3708–3720. doi:10.1039/D1LC00307K

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