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FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-RPMIH-B1X
$44.00
DCP-RPMIH-B1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium with 25mM HEPES, w/o Sodium Bicarbonate

Contains L-Glutamine Contains Phenol Red Contains 25mM HEPES Contains Calcium Contains Magnesium Contains 2.0 g/L Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-RPMIH-B1X is a Microfluidics Suitable, ultra-filtered RPMI 1640 + 25mM HEPES formulation engineered for hematopoietic cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides CO₂-independent pH buffering in place of a bicarbonate system.

  • RPMI 1640 base with 25 mM HEPES (pKa 7.3 at 37°C) — CO₂-independent pH buffering, sodium bicarbonate omitted from the formulation
  • D-Glucose at 2000 mg/L (2.0 g/L), L-Glutamine at 300 mg/L, and sodium pyruvate at 110 mg/L support extended hematopoietic and lymphocyte culture
  • Glutathione (reduced), 1.0 mg/L, included for antioxidant/redox support relevant to lymphocyte and NK cell culture
  • Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, four passes) reaching a 0.04 µm final polish
  • Endotoxin release specification < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • 41 formulation components verified per lot with CAS-level raw-material traceability
  • Customization of pH, HEPES concentration, and nutrient load available on request — contact support@diagnocine.com
CAT. NO.
DCP-RPMIH-B1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 + 25mM HEPES, w/o Sodium Bicarbonate — 1X Liquid
  • Media familyRPMI 1640 + 25mM HEPES
  • Glucose2000 mg/L (2.0 g/L)
  • HEPES25 mM, pKa 7.3 at 37°C
  • Formulation[+] L-Glutamine, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose, [+] Sodium Pyruvate | [-] Sodium Bicarbonate
  • AppearanceOrange-colored, clear solution (phenol red indicator present)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments capable of activating TLR4 in immune cell assays. FluxMPS™ is built to address these gaps at the filtration and QC level.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate testing. Suited to suspension immune cell OoC channels and flow cytometry workflows where subvisible particulates confound gating.

science

Immune cell–optimized formulation

RPMI 1640 base with reduced glutathione (1.0 mg/L) and a balanced amino acid/vitamin profile commonly used for lymphocyte, monocyte, and hematopoietic cancer cell culture.

water_drop

Ultrapure-grade water

Formulated with Type 1 water (18.2 MΩ·cm) under controlled trace-metal and total organic carbon (TOC) limits, minimizing chemical background in sensitive lymphocyte culture systems.

visibility

Low background for imaging

The 0.04 µm final filtration substantially reduces subvisible particulate baseline relative to 0.22 µm media, supporting confocal imaging and biosensor-based readouts with fewer particulate artifacts. This formulation contains phenol red, so it is not intended for autofluorescence-sensitive applications — a phenol red–free variant is available on request.

shield

Low endotoxin release specification

Released to < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch, helping reduce the risk of LPS-driven TLR4/NF-κB activation artifacts in T cell and NK cell assays.

tune

Customization on demand

pH, HEPES concentration, nutrient load, and other formulation parameters available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

DCP-RPMIH-B1X is processed through four serial filtration passes — a repeated prefilter-plus-final-filter pair, run twice — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Large particulate, cell debris, and protein aggregate removal; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a standard 0.22 µm filter would not.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2 effluent, but redundant protection for Stage 4.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate 0.04 µm polishing filtration; aseptic fill and finish in an ISO Class 5 (Class 100) environment.

Performance vs. conventional media

5×
Lower particulate counts vs. 0.22 µm media (by count)
0.04
µm final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is mitigated by 0.1 µm and 0.04 µm mycoplasma-retentive filtration (0.2–0.3 µm organisms); this is a filtration control, not a per-lot mycoplasma test result.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS RPMI 1640 with 25mM HEPES (DCP-RPMIH-B1X) Quadruple-stage filtration system diagram, 0.1 micron x2 plus 0.04 micron x2, for organ-on-a-chip and microfluidic cell culture applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIH-B1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-B1X — RPMI 1640 + 25mM HEPES, w/o sodium bicarbonate — provides 0.04 µm filtered, CO₂-independent medium for hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional MPS Grade, 0.01 µm (10 nm) ultra nano-filtered variant of this formulation is available on request for automated, valve-driven perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the 0.04 µm tier
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the MPS Grade 0.01 µm variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is a standard base for primary T cells, B cells, NK cells, and monocytes. The 0.04 µm filtration is intended to reduce particulate load relevant to TLR4-sensitive immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines commonly cultured in RPMI rather than DMEM-based media.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models where particulate-driven TLR4 activation is a concern.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

A low endotoxin release specification (< 0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols where endotoxin control is a process priority.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Defined RPMI base suitable for ¹³C isotope tracing and glycolysis flux studies of lymphocyte activation states. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium; this formulation contains phenol red.

¹³C tracingGlycolysisLC-MS metabolomics
Live-Cell Imaging

Flow Cytometry & Confocal

Reduced particulate baseline supports flow cytometry and confocal imaging workflows. A phenol red–free variant is available for autofluorescence-sensitive applications.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix below. Available pack sizes: 500 mL, 1000 mL. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose, [+] Sodium Pyruvate | [-] Sodium Bicarbonate
Appearance Orange-colored, clear solution (phenol red indicator present)
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H₂O
Total ingredients 41
Available pack sizes 500 mL, 1000 mL
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (release specification, per batch)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent — HEPES (25 mM) maintains pH without gas supplementation; sodium bicarbonate not present
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES, w/o sodium bicarbonate: 41 formulation components verified per lot with CAS numbers for raw-material traceability. Contains reduced glutathione and a balanced amino acid/vitamin profile commonly used for lymphocyte and hematopoietic cell culture.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.00
i-Inositol 87-89-8 35.000
Phenol red sodium salt 34487-61-1 5.300
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-B1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feedwater with controlled trace-metal and total organic carbon (TOC) levels.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow environment.

assignment

Micro-Batch Precision

Small-batch production with per-lot QC testing; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch; assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H₂O.

Documentation & CoA

Full Certificate of Analysis with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIH-B1X compares

FluxMPS™ DCP-RPMIH-B1X vs. conventional 0.22 µm–filtered RPMI formulations, and against published supplier endotoxin specifications.

Parameter DCP-RPMIH-B1X (FluxMPS™) Conventional RPMI 1640 + 25mM HEPES (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
Formulation trait HEPES-buffered, sodium bicarbonate-free, CO₂-independent Sodium bicarbonate–buffered, CO₂ incubator required Sodium bicarbonate–buffered, CO₂ incubator required
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1/0.04 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none specified ISO 9001 or none specified
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Higher clogging risk cancel Higher clogging risk
Custom formulation available check_circle Yes cancel Typically no cancel Typically no

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-B1X — RPMI 1640 + 25mM HEPES, w/o sodium bicarbonate.

Yes. DCP-RPMIH-B1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, and is classified Microfluidics Suitable. RPMI 1640 with this filtration profile is used in immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.
FluxMPS™ uses four sequential filtration passes: 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish).
Sodium bicarbonate is omitted so that 25 mM HEPES can provide CO₂-independent buffering, enabling fully atmospheric immune cell suspension culture for CFU assays, closed-system TIL expansion, and lymphocyte protocols run outside a CO₂ incubator. Glucose (2.0 g/L), L-glutamine (300 mg/L), and sodium pyruvate (110 mg/L) are already included in this formulation; no additional carbon source or buffering supplementation is required for standard RPMI-based protocols.
No. This formulation is CO₂-independent — 25 mM HEPES maintains pH without gas supplementation, since sodium bicarbonate is not present in the formulation.
Yes. FBS (typically 5–10%), serum-free supplements, growth factors, or antibiotics can be added as required. When adding serum or protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane rather than a 0.04 µm membrane, which would retain immunoglobulins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
FluxMPS™ DCP-RPMIH-B1X carries a release specification of < 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch rather than per unit. For immune cell assays, endotoxin/LPS can activate TLR4/NF-κB signaling and alter lymphocyte activation state; controlling endotoxin to this specification is intended to reduce that risk.
Yes. Full CoA per batch covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration control, particulate count (USP <788> Method 1), lot number, and expiry. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640-based immune cell culture and organ-on-a-chip applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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