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FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o Phenol Red: 1X Liquid

Product#: DCP-RPMIH-R1X
$44.00
DCP-RPMIH-R1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o Phenol Red — 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Phenol Red

FluxMPS™ DCP-RPMIH-R1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 + 25mM HEPES formulation engineered for hematopoietic cells, lymphocytes, and immune cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The train reaches a 0.04 µm final cut-off — 5.5× finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides robust pH buffering independent of CO2. Formulation: [+] 2 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 25mM HEPES | [-] Phenol Red.

  • Glucose source: 2000 mg/L (2.0 g/L) — supports standard hematopoietic and lymphocyte energy metabolism
  • 25 mM HEPES (pKa 7.3 at 37°C) — robust pH buffering during open-bench handling and imaging outside CO2 incubation
  • Sodium bicarbonate (2000 mg/L) retained alongside HEPES — dual-buffered system compatible with standard 5% CO2 incubators
  • Glutathione (reduced, 1 mg/L) included — antioxidant support for redox-sensitive immune cell types
  • Phenol red excluded — reduces phenol-red-associated background fluorescence for flow cytometry and imaging workflows
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off; endotoxin release specification < 0.05 EU/mL
  • Manufactured under an ISO 13485:2016 quality management system with per-lot Certificate of Analysis
CAT. NO.
DCP-RPMIH-R1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 + 25mM HEPES w/o Phenol Red — 1X Liquid
  • Glucose2000 mg/L (2.0 g/L)
  • L-Glutamine300 mg/L
  • Sodium Pyruvate110 mg/L
  • HEPES25 mM, pKa 7.3 at 37°C
  • Formulation[+] Glucose, [+] L-Glutamine, [+] Na Pyruvate, [+] Na Bicarbonate, [+] HEPES | [-] Phenol Red
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)280–320 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes subvisible particulates and mycoplasma-sized organisms that a finer filtration train can exclude. FluxMPS™ is built to close that gap for immune cell and hematopoietic culture models.

filter_alt

Microchannel-safe, low-endotoxin purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance. Endotoxin release specification < 0.05 EU/mL supports suspension immune cell OoC and flow cytometry work where particulate and endotoxin background confound results.

target

Immune cell–optimized formulation

RPMI 1640 base contains glutathione (reduced) and a balanced amino acid and vitamin profile suited to lymphocyte and hematopoietic cell culture.

water_drop

Trace-metal & TOC controlled water

Manufactured with Type 1 water (18.2 MΩ·cm), reducing trace-metal and organic-carbon (TOC) background contributed by the feed water to the finished medium.

visibility

Low background for imaging

Ultra-low particulate load from the quadruple-stage filtration train supports confocal microscopy and flow cytometry workflows with reduced particulate interference.

science

HEPES pH stability

25 mM HEPES (pKa 7.3 at 37°C) buffers pH during open-bench handling, flow cytometry preparation, and imaging sessions outside CO2 incubation.

tune

Customization on demand

pH, nutrient concentrations, HEPES level, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, alternating a 0.1 µm prefilter with a 0.04 µm final filter, reach a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; applies 0.1 µm-class mycoplasma-retentive filtration and retains sub-micron particulates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second, dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2 effluent.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

The quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off — 5.5× finer than the 0.22 µm membranes used for conventional sterile filtration — and applies 0.1 µm mycoplasma-retentive filtration at two points in the process.

5.5×
Finer final pore size vs. 0.22 µm conventional filtration
0.04
µm Final pore size — sub-mycoplasma-scale polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS RPMI 1640 with 25mM HEPES DCP-RPMIH-R1X quadruple-stage filtration system diagram, 0.1 micron x2 plus 0.04 micron x2, for organ-on-a-chip and microfluidic cell culture applications - Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIH-R1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-R1X — RPMI 1640 + 25mM HEPES, phenol red-free — delivers 0.04 µm filtered purity for hematopoietic cell and related microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates below the 0.04 µm cut-off of this Microfluidics Suitable product
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes. The 0.04 µm filtration train targets particulate levels that can confound sensitive immune cell assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 hematopoietic cancer lines, Jurkat, Raji, K562, and HL-60, where DMEM-based formulations would alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models built on microfluidic and microphysiological system (MPS) platforms.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Endotoxin release specification < 0.05 EU/mL supports CAR-T manufacturing and TIL expansion protocols where endotoxin control is a process requirement.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium; this formulation retains sodium bicarbonate. Suited instead to off-line glycolysis/OXPHOS flux measurements and ¹³C isotope tracing of lymphocyte activation states.

¹³C tracingGlycolysis flux
Live-Cell Imaging

Flow Cytometry & Confocal

The phenol red-free formulation removes phenol-red-associated background fluorescence in FITC/PE detection channels; ultra-low particulate levels further reduce light-scatter noise in flow cytometry and confocal imaging.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 2 g/L Glucose, [+] L-Glutamine (300 mg/L), [+] Sodium Pyruvate (110 mg/L), [+] Sodium Bicarbonate (2000 mg/L), [+] 25 mM HEPES | [-] Phenol Red
Appearance Colorless to pale yellow, clear solution
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 280–320 mOsm/kg H2O
Total ingredients 41 components across 4 categories
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification — see Manufacturing & QA)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 recommended; sodium bicarbonate (2000 mg/L) provides primary buffering, supplemented by 25 mM HEPES for pH stability during ambient handling
Pack sizes 500 mL, 1000 mL
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 41 ingredients verified per lot with CAS numbers for full raw-material traceability. RPMI 1640 contains glutathione (antioxidant) and a balanced profile of vitamins and amino acids suited to lymphocyte and hematopoietic cell culture.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2000.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.000
Sodium Pyruvate 113-24-6 110.000
i-Inositol 87-89-8 35.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-R1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016 quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water reduces trace-metal and organic-carbon (TOC) background in the finished medium.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch (assay sensitivity 0.005 EU/mL).

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 280–320 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIH-R1X compares

FluxMPS™ DCP-RPMIH-R1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMIH-R1X (FluxMPS™) Conventional RPMI 1640 + 25mM HEPES (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
RPMI 1640 + 25mM HEPES, phenol red-free — reduced background fluorescence base check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
0.1 µm mycoplasma-retentive filtration check_circle Yes cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes (0.04 µm filtered) cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-R1X — RPMI 1640 + 25mM HEPES, phenol red-free.

Yes. DCP-RPMIH-R1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, applying 0.1 µm mycoplasma-retentive filtration for ultra-low particulate purity suited to MPS, OoC, and LoC platforms. RPMI 1640 is the standard base for immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.
FluxMPS™ uses four sequential filters: 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish). The resulting 0.04 µm final cut-off is 5.5× finer than the 0.22 µm membranes used in conventional sterile filtration, with 0.1 µm mycoplasma-retentive filtration applied at two points in the train.
Phenol red is removed to reduce phenol-red-associated background fluorescence in flow cytometry (particularly FITC/PE channels) and confocal imaging. HEPES (25 mM) maintains pH stability during imaging sessions outside CO2 incubators, eliminating the need for a colorimetric pH indicator. No other component is substituted — supplement per your standard protocol (e.g., serum, growth factors) as described below.
5% CO2 is recommended. Sodium bicarbonate (2000 mg/L) provides the primary buffering system at that CO2 level, while the 25 mM HEPES supplement maintains pH stability during handling outside the incubator.
Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. When filtering serum-containing or protein-containing supplements before addition, use a 0.2 µm low-protein-binding PES or PVDF syringe filter — a 0.04 µm membrane is not appropriate for this purpose, as it retains IgM, lipoproteins, and much of the functional protein fraction of serum. Contact support@diagnocine.com for custom co-formulation.
The release specification is < 0.05 EU/mL, verified by LAL assay per USP <85> (assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch, with every batch tested before release; a Certificate of Analysis is available on request.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 + 25mM HEPES in immune cell culture and organ-on-a-chip applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1001/jama.1967.03120080053007
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Junkin M, et al. High-content quantification of single-cell immune dynamics. Cell Rep. 2016;15:411–422. doi:10.1016/j.celrep.2016.03.033
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Ando Y, et al. Engineering a vascularized lymph node-on-chip. Adv Healthc Mater. 2019;8:1900103. doi:10.1002/adhm.201900103
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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