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FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-RPMIH-QBR1X
$44.00
DCP-RPMIH-QBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 + 25mM HEPES

Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains Glucose (2.0 g/L) Contains Sodium Pyruvate (110 mg/L) Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-RPMIH-QBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 + 25 mM HEPES formulation engineered for hematopoietic cells, lymphocytes, and related immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The 0.04 µm final cut-off is five times finer than the 0.22 µm membranes used in conventional sterile filtration of RPMI media. HEPES (25 mM, pKa 7.3 at 37°C) provides CO₂-independent pH buffering, and the bicarbonate-free, phenol-red-free formulation supports open-bench handling and fluorescence-based immune assays. Formulation: [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L), [+] Sodium Pyruvate (110 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red.

  • Glucose source retained: 2000 mg/L (2.0 g/L) — full carbon source despite HEPES-only buffering
  • 25 mM HEPES (pKa 7.3 at 37°C) provides robust, CO₂-independent pH buffering for open-bench and imaging workflows
  • Glutathione (reduced), 1.000 mg/L — antioxidant support for redox-sensitive immune cell types
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL by LAL assay (USP <85>)
  • Bicarbonate-free and phenol-red-free — reduces autofluorescence background for PE-channel flow cytometry and confocal imaging
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom pH, nutrient, and HEPES concentration modifications available on request
CAT. NO.
DCP-RPMIH-QBR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 + 25mM HEPES — 1X Liquid
  • Media familyRPMI 1640 + 25mM HEPES
  • Glucose2000 mg/L (2.0 g/L)
  • HEPES25 mM (pKa 7.3 at 37°C)
  • Formulation[+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L), [+] Sodium Pyruvate (110 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes subvisible particulates and mycoplasma-sized bioburden that standard filtration cannot retain. FluxMPS™ DCP-RPMIH-QBR1X is built around a validated quadruple-stage filtration train and a formulation tailored for immune-cell and hematopoietic culture.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance testing supports suspension immune-cell OoC and microfluidic channel applications.

target

Defined metabolic profile

2.0 g/L glucose and 110 mg/L sodium pyruvate are held to a fixed, released specification, supporting reproducible metabolic flux and glycolysis studies in lymphocyte and cancer cell lines.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) supports low trace-metal and low-TOC formulation control during manufacture.

visibility

Low background for imaging

0.04 µm filtration reduces the particulate baseline for confocal and biosensor imaging. Note: riboflavin (0.200 mg/L) contributes intrinsic media autofluorescence near 525 nm, independent of filtration — account for this in fluorescence assay design.

science

Rich, stable nutrient profile

19 amino acids, 11 vitamins, and glutathione (reduced antioxidant) formulated to a fixed release specification and produced under micro-batch, per-lot QC.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration passes — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter would pass.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish in validated ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

FluxMPS™ DCP-RPMIH-QBR1X is processed through a quadruple-stage filtration train reaching a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional sterile filtration of RPMI 1640 media.

0.04
µm final filtration pore size — five times finer than 0.22 µm conventional media
4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk (organism diameter 0.2–0.3 µm) is mitigated by 0.1 µm mycoplasma-retentive filtration at each prefiltration stage; this is a filtration-based risk-mitigation control and is not a per-lot mycoplasma test result.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMIH-QBR1X RPMI 1640 + 25mM HEPES quadruple-stage filtration system diagram: 0.1 micron x2 + 0.04 micron x2 filtration for organ-on-a-chip and microfluidic cell culture media, Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) used to manufacture DCP-RPMIH-QBR1X.
© Diagnocine® — DCP-RPMIH-QBR1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-QBR1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered, Microfluidics Suitable media for hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion systems.

  • Extended particulate exclusion: 0.01 µm filtration targets nanoparticulate aggregates beyond the 0.04 µm cut-off
  • Valve & sensor protection: reduces micro-fouling risk in automated perfusion systems
  • Extended perfusion stability: supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration lowers the particulate baseline relevant to immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM formulation would alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph-node-on-chip platforms.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Low endotoxin release specification (< 0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols where endotoxin can activate TLR4 signaling in T cells.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Bicarbonate-free, phenol-red-free base compatible with Agilent Seahorse XF T cell metabolic assays, glycolysis stress tests, and ¹³C isotope tracing of lymphocyte activation states.

Seahorse XF¹³C tracingGlycolysis
Live-Cell Imaging

Flow Cytometry & Confocal

Phenol-red-free formulation reduces background absorbance for PE-channel flow cytometry and confocal imaging of immune cells; see the imaging note in Why FluxMPS™ regarding riboflavin autofluorescence.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Available sizes: 500 mL, 1000 mL. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (2.0 g/L), [+] Sodium Pyruvate (110 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow, clear solution (phenol red-free)
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H₂O
Total ingredients 39
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent — 25 mM HEPES buffering maintains pH stability without a CO₂ incubator
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 39 ingredients verified per lot with CAS numbers for full raw-material traceability. Contains glutathione (reduced antioxidant) and a balanced profile of amino acids and vitamins optimized for lymphocyte and hematopoietic cell culture.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.00
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-QBR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016 quality management system. Final QC at Diagnocine, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — supports low trace-metal, low-TOC formulation control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis available for every batch.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIH-QBR1X compares

FluxMPS™ DCP-RPMIH-QBR1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMIH-QBR1X (FluxMPS™) Conventional RPMI 1640 + HEPES
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
RPMI 1640 + HEPES-only, CO₂-free — no glutamine, no phenol red check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration passes 4 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stages) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-QBR1X — RPMI 1640 + 25mM HEPES.

Yes. DCP-RPMIH-QBR1X is a Microfluidics Suitable RPMI 1640 + 25mM HEPES formulation processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size. It is suited to immune-cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models on microphysiological system (MPS) platforms.
FluxMPS™ uses four sequential passes — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional sterile filtration.
L-Glutamine is omitted so it can be added fresh at time of use, since it degrades in liquid storage. Sodium bicarbonate is omitted because 25 mM HEPES independently maintains pH without CO₂. Phenol red is omitted to reduce background absorbance for fluorescence and colorimetric assays. Add L-glutamine (2–4 mM) or a stabilized dipeptide substitute, and sodium bicarbonate only if a CO₂ incubator workflow is required. Contact support@diagnocine.com for guidance.
No. This formulation is bicarbonate-free and HEPES-buffered (25 mM), so it is CO₂-independent and maintains pH stability at 37°C without a CO₂ incubator.
Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. Filter serum-containing or protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane — never 0.04 µm, which retains serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
DCP-RPMIH-QBR1X is manufactured to a release specification of < 0.05 EU/mL, tested per batch by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Every batch is tested before release and must meet this specification; a Certificate of Analysis is available on request.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 + 25mM HEPES in immune cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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