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FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Product#: DCP-RPMIH-QB1X
$44.00
DCP-RPMIH-QB1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 + 25mM HEPES

Contains Phenol Red Contains 25mM HEPES Contains Calcium Contains Magnesium Contains 2.0 g/L Glucose Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate

FluxMPS™ DCP-RPMIH-QB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 + 25mM HEPES formulation engineered for hematopoietic cells, lymphocytes and related immune cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The quadruple-stage train reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides CO2-independent pH buffering; this formulation excludes L-glutamine and sodium bicarbonate so users can control nitrogen source and buffering strategy at time of use.

  • Carbon source: 2.0 g/L (2000 mg/L) D-glucose, plus 110 mg/L sodium pyruvate
  • 25 mM HEPES (pKa 7.3 at 37°C) — CO2-independent pH buffering for open-air handling and flow cytometry preparation
  • Glutathione (reduced), 1.0 mg/L — antioxidant support for redox-sensitive lymphocyte populations
  • Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
  • L-glutamine and sodium bicarbonate excluded — add fresh glutamine and select a buffering strategy suited to your culture system
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Contains phenol red (5.3 mg/L) — a phenol red-free variant is available on request for fluorescence-sensitive imaging
CAT. NO.
DCP-RPMIH-QB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 + 25mM HEPES — 1X Liquid
  • Media familyRPMI 1640 + 25mM HEPES
  • Formulation[+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Sodium Bicarbonate
  • AppearanceOrange-red, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230-270 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • GradeMicrofluidics Suitable (0.04 µm final cut-off)
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that can activate TLR4 signaling and confound immune cell assays. FluxMPS™ is built to mitigate these failure modes at the filtration stage.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance — low-particulate media for suspension immune cell OoC and flow cytometry workflows.

science

Immune cell-optimized formulation

RPMI 1640 contains reduced glutathione (antioxidant) and a balanced profile of vitamins and amino acids historically optimized for lymphocyte and hematopoietic cell culture.

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Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm, with low trace-metal and organic carbon content — supports consistent, reproducible culture conditions lot to lot.

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Low endotoxin release specification

< 0.05 EU/mL, tested per batch by LAL (USP <85>) — supports assays sensitive to LPS-driven TLR4/NF-κB activation in T cells and NK cells.

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HEPES pH stability

25 mM HEPES (pKa 7.3 at 37°C) resists pH drift during open-air handling, flow cytometry preparation, and non-incubator workflows.

edit_note

Customization on demand

pH, nutrient concentrations, HEPES level, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a 0.04 µm final pore size under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates not captured by standard 0.22 µm membranes.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge and providing 0.1 µm mycoplasma-retentive filtration.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish performed in validated ISO Class 5 (Class 100) laminar-flow workstations.

Performance vs. conventional media

FluxMPS™ DCP-RPMIH-QB1X is processed through a validated quadruple-stage filtration train reaching a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional filtration — with 0.1 µm mycoplasma-retentive prefiltration at two points in the process.

5×
Finer final cut-off than 0.22 µm conventional media (0.04 µm vs 0.22 µm)
4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk (organisms 0.2-0.3 µm in diameter) is mitigated through 0.1 µm mycoplasma-retentive filtration; mycoplasma testing is not performed on every lot.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS RPMI 1640 plus 25mM HEPES (DCP-RPMIH-QB1X) quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 for organ-on-a-chip, microfluidic and immune cell culture applications, Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIH-QB1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-QB1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered, Microfluidics Suitable media for hematopoietic and immune cell OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) six-stage ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion systems and robotics-driven culture platforms.

  • Total Particulate Exclusion: 0.01 µm filtration targets nanoparticulate aggregates beyond the standard 0.04 µm cut-off
  • Valve & Sensor Protection: Designed to reduce micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is a standard base for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration reduces particulate load that can confound sensitive immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and other hematopoietic cancer lines where DMEM-based media can alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip models where low-particulate media supports sensitive immune readouts.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Low endotoxin release specification (<0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols where LPS-driven T cell activation artefacts are a concern.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium. This HEPES-buffered, bicarbonate-free RPMI base supports defined-substrate flux studies and isotope tracing of lymphocyte activation states outside the XF platform.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Flow Cytometry & Confocal Imaging

0.04 µm final filtration supports low-particulate media for flow cytometry and confocal imaging workflows. This formulation contains phenol red (5.3 mg/L); a phenol red-free variant is available on request for GFP and fluorescence-sensitive imaging.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA available on request: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2.0 g/L Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Sodium Bicarbonate
Appearance Orange-red, clear solution
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
Sodium Pyruvate 110 mg/L
pH USP <791> 7.4
Osmolality USP <785> 230-270 mOsm/kg H2O
Total ingredients 40 across 4 source categories
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — 25 mM HEPES alone maintains pH 7.2-7.4 at 37°C
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Available pack sizes: 500 mL, 1000 mL. Contact support@diagnocine.com for custom fill volumes or pricing.
Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 40 ingredients verified per lot with CAS numbers for full raw-material traceability. RPMI 1640 contains reduced glutathione and a balanced profile of vitamins and amino acids historically optimized for lymphocyte and hematopoietic cell culture.

INORGANIC SALTS
Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
AMINO ACIDS
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
VITAMINS AND OTHERS
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.00
Phenol red sodium salt 34487-61-1 5.300
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-QB1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm, low trace-metal and organic carbon content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230-270 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-RPMIH-QB1X lot at support@diagnocine.com.
Product Comparison

How DCP-RPMIH-QB1X compares

FluxMPS™ DCP-RPMIH-QB1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMIH-QB1X (FluxMPS™) Conventional RPMI 1640 + HEPES (0.22 µm filtered) Standard DMEM/RPMI (0.22 µm filtered)
Grade Microfluidics Suitable Standard 0.22 µm filtered Standard 0.22 µm filtered
HEPES-buffered, bicarbonate-free, fresh-glutamine formulation for atmospheric immune cell suspension check_circle Yes cancel Usually no cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
0.1 µm mycoplasma-retentive filtration check_circle Yes cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes — Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-QB1X — RPMI 1640 + 25mM HEPES.

Yes. DCP-RPMIH-QB1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, making it Microfluidics Suitable for MPS, OoC, and LoC platforms. RPMI 1640 with 0.1 µm mycoplasma-retentive filtration is suited to immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models.
FluxMPS™ uses four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional filtration, with 0.1 µm mycoplasma-retentive prefiltration at two points in the process.
This formulation is HEPES-buffered and bicarbonate-free by design, so buffering is CO2-independent. L-glutamine is left out because it degrades in liquid storage; add fresh L-glutamine (commonly 2 mM) or a stabilized dipeptide substitute at time of use, tailored to your cell type's nitrogen requirements.
No. This formulation is CO2-independent — 25 mM HEPES alone maintains pH 7.2-7.4 at 37°C, making it suitable for atmospheric handling and open-bench workflows.
Yes. Add FBS (typically 5-10%), serum-free supplements, growth factors, or antibiotics as required. When adding serum or protein-containing supplements, use a 0.2 µm low-protein-binding PES or PVDF filter (never 0.04 µm, which retains IgM, VLDL, and much of the lipid/lipoprotein fraction of serum). Contact support@diagnocine.com for custom co-formulation.
Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before it ships. Endotoxin is controlled per manufacturing batch rather than per unit. For immune cell assays, endotoxin above typical thresholds can activate TLR4/NF-κB, inducing cytokines and altering lymphocyte activation state independently of experimental conditions.
Yes. A full CoA per lot covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 + 25mM HEPES in immune cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519-524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407-420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119-1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588-3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446-452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201-1212. doi:10.1039/c3lc41017j

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