FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. This high-glucose (4.5 g/L) RPMI 1640 base is supplied without L-glutamine, sodium pyruvate, or phenol red so glutamine source, energy substrate, and optical background can each be defined by the end user.

• High-glucose RPMI 1640 base: 4500 mg/L (4.5 g/L) D-glucose, formulated without L-glutamine, sodium pyruvate, and phenol red for full user control of carbon source and optical background
• Quadruple-stage nano-filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II), reaching a 0.04 µm final cut-off
• Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> Bacterial Endotoxins Test)
• pH 7.4 (USP <791>); sodium bicarbonate buffering (2000 mg/L) supports a 5% CO₂ atmosphere
• Prepared with Type 1 water (18.2 MΩ·cm) under an ISO 13485:2016 quality management system
• Micro-batch precision manufacturing with full lot traceability and Certificate of Analysis available on request
• Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
• Manufactured and released at Diagnocine, Totowa, NJ

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized organisms (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings, triggering inflammation in primary cultures, and shortening device lifetimes. FluxMPS™ is built to address these failure modes at the filtration stage.

Quadruple-stage filtration system

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is processed through a validated four-stage filtration train — two paired 0.1 µm prefilter / 0.04 µm final-filter stages run in series — reaching a 0.04 µm final cut-off. This retains mycoplasma-sized organisms and subvisible particulates that pass through conventional 0.22 µm filtration.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm final filter cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter would not capture.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2 effluent, but redundant protection for the final filter.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter prior to ISO Class 5 aseptic fill & finish.

Designed for next-generation cell culture platforms

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid is intended for organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) MPS Grade variant — a separate six-stage ultra nano-filtered line beyond this Microfluidics Suitable product — is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

• Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
• Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
• Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade version.

Lot-release quality parameters

Every production lot of FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid undergoes the complete quality-release battery below before shipment.

Full composition (mg/L)

Complete formulation with CAS numbers — 38 components across 4 categories. All ingredient names and mg/L values reproduced from manufacturer specification. Custom compositions available on request.

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS, with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

How DCP-RPMIG-QPR1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as “Not specified”.

Frequently asked questions

Common questions about FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Sodium Pyruvate, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.

Supporting literature

Peer-reviewed publications supporting the scientific rationale for Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.

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3. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
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5. Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
6. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
7. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
8. Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
9. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175
10. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539