FluxMPS RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid

Q: What makes the filtration process superior to standard 0.22 micron filtered media?

Standard 0.22 micron filtration does not retain mycoplasma (0.2-0.3 micron) or subvisible particulates that accumulate in microchannels. FluxMPS uses four sequential stages reaching 0.04 micron, delivering approximately 5x lower particulate counts per USP 788.

Q: Can DCP-RPMIG-QR1X be supplemented with serum, growth factors, or other components?

Yes. Can be supplemented with FBS (5-20%), growth factors, antibiotics, or other additives per standard practice. When adding serum or protein-containing supplements, pre-filter using a 0.2 micron low-protein-binding PES or PVDF membrane; the medium's own 0.04 micron final-stage filtration is not intended for post-supplementation filtration of serum-containing formulations. Add supplements immediately before use.

Q: Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. A batch-specific CoA is available for every shipment and includes appearance, pH (USP 791), osmolality (USP 785), endotoxin (USP 85 BET), sterility (USP 71), particulate matter (USP 788), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.

ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid

Sodium Bicarbonate Calcium Magnesium Glucose Sodium Pyruvate L-Glutamine Phenol Red HEPES

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

Quadruple-stage filtration: paired 0.1 µm prefilters and 0.04 µm final filters, two full passes, for microchannel-safe purity
Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> BET), tested per manufacturing batch
RPMI 1640 base formulation, High Glucose (4500 mg/L); pH 7.4 (USP <791>)
L-Glutamine and phenol red excluded from this formulation — supplement L-Glutamine or GlutaMAX™ as needed at time of use
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for low trace-metal and organic-carbon background
ISO Class 5 aseptic fill & finish; manufactured under an ISO 13485:2016 quality management system
Mycoplasma risk mitigated via dedicated 0.1 µm mycoplasma-retentive filtration stages (not tested per lot)
Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request

DCP-RPMIG-QR1X | Cell Culture Media Size: 500 mL and 1000 mL UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid

Glucose4500 mg/L (4.5 g/L)
L-GlutamineNot present — supplement as needed
Sodium Pyruvate110 mg/L
Phenol RedNot present
pH (USP <791>)7.4
Osmolality (USP <785>)See Certificate of Analysis
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Storage2–8°C, protect from light
Shelf Life12 months from date of manufacture, unopened

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that can accumulate inside microchannels — corrupting biosensor readings, stressing primary cultures, and shortening device lifetimes. FluxMPS™ is engineered to address these failure modes through a validated quadruple-stage filtration train.

filter_alt

Microchannel-safe purity

Quadruple-stage filtration reaching a 0.04 µm final pore size, including dedicated 0.1 µm mycoplasma-retentive stages; USP <788> particulate compliance verified per lot.

target

Total metabolic control

High-glucose, L-glutamine-free base formulation gives researchers direct control over nitrogen source timing for metabolic flux experiments and Warburg-pathway studies.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm), controlled for low trace-metal and organic-carbon (TOC) content.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy, live-cell biosensors, and TEER measurements where particulate scatter must be minimized.

science

Rich, stable nutrient profile

Micro-batch precision manufacturing locks in amino acid and vitamin concentrations for lot-to-lot reproducibility in long-term perfusion studies.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid is processed through a four-stage filtration train — two paired 0.1 µm prefilter / 0.04 µm final-filter passes — reaching a 0.04 µm final pore size, well below mycoplasma size and the subvisible particulates that 0.22 µm filtration cannot address.

1

0.1 µm Prefiltration I

Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm final-filter cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass through a 0.22 µm filter.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge from breakthrough load.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter; ISO Class 5 aseptic fill and finish in a validated laminar-flow workstation.

Performance vs. conventional media

Two paired prefilter / final-filter passes reaching a 0.04 µm final pore size deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> compliance verified on every lot.

5×

0.04

µm final filter pore size — sub-mycoplasma polishing

Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is mitigated by dedicated 0.1 µm mycoplasma-retentive filtration stages; this is a filtration control, not a per-lot mycoplasma assay.

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid (DCP-RPMIG-QR1X) Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II for organ-on-a-chip and microfluidic applications by Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIG-QR1X

Applications

Designed for next-generation cell culture platforms

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid is validated for organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available for automated bioreactor and robotic perfusion systems requiring the highest particulate exclusion.

Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation supports microchannel patency and laminar flow integrity.

OoCToCBoCLoCMPS

Cancer Biology

Warburg Effect & Metabolic Research

High-glucose, glutamine-free base with a controlled endotoxin release specification supports precise metabolic flux analysis.

MCF-7MDA-MB-231HeLaA549

Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurement.

HUVECsHAECsPrimary hepatocytes

Metabolomics

Metabolic Flux Analysis

Chemically defined, glutamine-free base supports isotope tracing (¹³C) and NMR metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium formats.

¹³C tracingNMR metabolomics

Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load supports high-content confocal imaging and biosensor applications.

ConfocalBiosensorsTEER

Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters

Parameter	Specification
Formulation	High Glucose (4500 mg/L); Sodium Bicarbonate (2000 mg/L); Sodium Pyruvate (110 mg/L); L-Glutamine and Phenol Red not added
Appearance	Pale yellow to colorless, clear solution (phenol red-free)
pH USP <791>	7.4
Osmolality USP <785>	See Certificate of Analysis
Glucose	4500 mg/L (4.5 g/L)
L-Glutamine	Not present — supplement as needed
Sodium Pyruvate	110 mg/L
Phenol Red	Not present

Sterility, Purity & Safety Parameters

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL (batch release specification — see Manufacturing & Compliance)
Sterility USP <71>	No growth after 14 days
Mycoplasma	0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	Compliant
Particulate ≥25 µm USP <788> Method 1	Compliant
Water Purity Type 1	Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, protected from light
Freeze-thaw	Not recommended
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	5% CO₂ (derived from 2000 mg/L sodium bicarbonate buffering at pH 7.4)

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Cell culture / reagent grade
Traceability	Full lot documentation, CoA available
Manufacturing QMS	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch precision manufacturing
Intended use	For Research Use Only (RUO)

Formulation

Full composition (mg/L)

Complete formulation with CAS numbers. Total: 39 components across 4 categories. All ingredient names and mg/L values reproduced from manufacturer specification. Custom compositions available on request.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate	13477-34-4	100.000
Magnesium sulfate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	2000.000
Sodium chloride	7647-14-5	6000.00
Sodium phosphate dibasic anhydrous	7558-79-4	800.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	10.000
L-Arginine hydrochloride	1119-34-2	241.000
L-Asparagine	70-47-3	50.000
L-Aspartic acid	56-84-8	20.000
L-Cystine dihydrochloride	30189-89-0	65.200
L-Glutamic acid	56-86-0	20.000
L-Histidine hydrochloride monohydrate	5934-29-2	20.960
L-Hydroxyproline	51-35-4	20.000
L-Isoleucine	73-32-5	50.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	40.000
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	15.000
L-Proline	147-85-3	20.000
L-Serine	56-45-1	30.000
L-Threonine	72-19-5	20.000
L-Tryptophan	73-22-3	5.000
L-Tyrosine Disodium Salt	69847-45-6	28.830
L-Valine	72-18-4	20.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	3.000
D-Biotin	58-85-5	0.200
D-Ca-Pantothenate	137-08-6	0.250
Folic acid	59-30-3	1.000
Niacinamide	98-92-0	1.000
Pyridoxine hydrochloride	58-56-0	1.000
Riboflavin	83-88-5	0.200
Thiamine hydrochloride	67-03-8	1.000
Vitamin B12	68-19-9	0.005
p-Amino benzoic acid (PABA)	150-13-0	1.000
OTHERS
D-Glucose	50-99-7	4500.000
Glutathione reduced	70-18-8	1.000
Sodium Pyruvate	113-24-6	110.000
i-Inositol	87-89-8	35.000

Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.

Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS, with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity Type 1 water, controlled for trace-metal and organic-carbon (TOC) content.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay on every batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance on every lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result reported on the Certificate of Analysis.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, raw material traceability, and release signatures for every lot.

Certificate of Analysis: Request via support@diagnocine.com with your lot number.

Product Comparison

How DCP-RPMIG-QR1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter	DCP-RPMIG-QR1X (FluxMPS™)	Conventional RPMI 1640 (0.22 µm)	Standard RPMI 1640 alternative
Grade	Microfluidics Suitable	Standard filtration (0.22 µm)	Standard filtration (0.22 µm)
Base Formulation	RPMI 1640, High Glucose w/o L-Glutamine, Phenol Red	RPMI 1640 Standard	RPMI 1640 Equivalent
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 stages	1 stage	1–2 stages
Mycoplasma-retentive filtration	check_circle	cancel	cancel
Endotoxin (release specification)	FluxMPS™ — < 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance	check_circle USP <788>	cancel	cancel
Water quality	Ultrapure Type 1 (18.2 MΩ)	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	Variable	Variable
Microfluidic channel compatibility	check_circle Validated	cancel Risk of clogging	cancel Risk of clogging
Custom formulation	check_circle On request	cancel	Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.

Is DCP-RPMIG-QR1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-RPMIG-QR1X uses our quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), delivering ultra-low particulate counts that support microchannel patency in OoC and MPS devices.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

Standard 0.22 µm filtration does not retain mycoplasma (0.2–0.3 µm) or subvisible particulates that accumulate in microchannels. FluxMPS™ uses four sequential stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788>.

Why is L-Glutamine excluded, and how do I supplement for my cell type?expand_more

L-Glutamine is unstable in liquid storage and degrades over time, so it is left out of this formulation and added fresh at time of use. Supplement with 2–4 mM L-Glutamine or a stabilized dipeptide such as GlutaMAX™ per your cell line's requirements; sodium pyruvate (110 mg/L) is already included as an alternate carbon/energy source.

Does this medium require a CO₂ incubator?expand_more

Yes. This formulation contains 2000 mg/L sodium bicarbonate, which buffers to pH 7.4 at approximately 5% CO₂. A standard 5% CO₂ incubator is recommended.

Can DCP-RPMIG-QR1X be supplemented with serum, growth factors, or other components?expand_more

Yes. FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid can be supplemented with FBS (5–20%), growth factors, antibiotics, or other additives per standard practice. When adding serum or protein-containing supplements, pre-filter using a 0.2 µm low-protein-binding PES or PVDF membrane; the medium's own 0.04 µm final-stage filtration is not intended for post-supplementation filtration of serum-containing formulations. Add supplements immediately before use.

What endotoxin level should I expect, and how is it verified?expand_more

Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> BET and must meet the release specification of < 0.05 EU/mL before shipment. Batch-specific results are documented in the Certificate of Analysis, available from support@diagnocine.com.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788>), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.

Scientific References

Supporting literature

Peer-reviewed publications supporting the scientific rationale for ultra-filtered media and microfluidic cell culture applications.

Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175
Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid

FluxMPS™ RPMI 1640 Medium, High Glucose w/o L-Glutamine, Phenol Red: 1X Liquid

Engineered where standard media fails

Microchannel-safe purity

Total metabolic control

Ultrapure-grade water

Low background for imaging

Rich, stable nutrient profile

Customization on demand

Quadruple-stage filtration system

0.1 µm Prefiltration I

0.04 µm Final filtration I

0.1 µm Prefiltration II

0.04 µm Final filtration II — Polish

Performance vs. conventional media

Designed for next-generation cell culture platforms

Automated Bioreactors & Robotics

Micro Physiological System (MPS) & Chip

Warburg Effect & Metabolic Research

iPSC-Derived Models

Endothelial & Primary Cells

Metabolic Flux Analysis

Microscopy & Optical Sensing

Lot-release quality parameters

Full composition (mg/L)

ISO 13485:2016 manufacturing & compliance

ISO 13485:2016 QMS

Ultrapure Type 1 Water

ISO Class 5 Fill & Finish

Micro-Batch Precision

Endotoxin — USP <85> BET

Particulate — USP <788> Method 1

Osmolality — USP <785>

Documentation — CoA & Full Lot Records

How DCP-RPMIG-QR1X (FluxMPS™) compares

Frequently asked questions

Supporting literature

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