Does this medium require a CO2 incubator?

This formulation contains 2000 mg/L (23.8 mM) sodium bicarbonate as its primary buffer, plus 25 mM HEPES as a secondary buffer. The bicarbonate system is calibrated for approximately 5% CO2 to maintain pH 7.4; incubate at 37 degC, 5% CO2. The added HEPES provides extra pH stability during open-bench handling outside the incubator.

FluxMPS RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid

Q: Is DCP-RPMIGH-P1X suitable for organ-on-a-chip (OoC) applications?

Yes. DCP-RPMIGH-P1X is a Microfluidics Suitable formulation engineered for MPS and OoC platforms. If your protocol requires pyruvate, add sodium pyruvate (typically 100-110 mg/L final) directly to the medium at time of use; filter any stock solution at 0.2 um for protein-containing additions or 0.1 um for defined, protein-free additions.

Q: Can DCP-RPMIGH-P1X be supplemented with serum, growth factors, or other components?

Yes. This is a basal medium formulation. Supplement with FBS, serum replacements, growth factors, or other additives as required by your cell type and protocol. Filter serum or protein-containing supplements at 0.2 um using a low-protein-binding PES or PVDF membrane; a 0.04 um membrane is not appropriate for serum. Defined, protein-free additions may be filtered at 0.1 um.

Q: What endotoxin level should I expect, and how is it verified?

Endotoxin is controlled per manufacturing batch to a release specification of less than 0.05 EU/mL, verified by the Limulus Amebocyte Lysate (LAL) assay per USP (Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) before each batch is released.

verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid

L-Glutamine Sodium Bicarbonate Phenol Red 25mM HEPES Calcium Magnesium High Glucose Sodium Pyruvate

FluxMPS™ DCP-RPMIGH-P1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid RPMI 1640 formulated with high glucose (4500 mg/L), 25 mM HEPES and sodium bicarbonate, without sodium pyruvate. It is engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms where microchannel-safe purity matters.

Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
High glucose (4500 mg/L) RPMI 1640 base with 25 mM HEPES and sodium bicarbonate dual buffering
Formulated without sodium pyruvate — add back per your protocol if required
Manufactured under an ISO 13485:2016 quality management system with full lot traceability
Type 1 ultrapure water (18.2 MΩ·cm) with controlled trace-metal and TOC content
ISO Class 5 (Class 100) aseptic fill & finish
pH 7.4; customizable glucose, HEPES, salts and nutrient composition on request

CAT. NO.

DCP-RPMIGH-P1X | Cell culture media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid

Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose, [-] Sodium Pyruvate
Glucose4500.000 mg/L
pH (USP <791>)7.4
Osmolality (USP <785>)Contact for specification
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Storage2–8°C, away from light
Shelf Life12 months from date of manufacture, unopened
ShippingCold pack
Pack Sizes500 mL, 1000 mL

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that can confound sensitive on-chip and imaging assays.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Reduced-particulate media for OoC microfluidic chips.

target

Total metabolic control

High-glucose, HEPES-supplemented formulation without sodium pyruvate for precise control of carbon-source inputs in your experimental system.

water_drop

Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm resistivity, with controlled trace-metal and total organic carbon (TOC) content at the point of formulation.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal, biosensor, and live-cell imaging applications on-chip.

science

Rich, stable nutrient profile

40 verified ingredients per lot. Full CAS traceability. Micro-batch precision manufacturing.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter/final-filter pairs — reach a final 0.04 µm polish under ISO Class 5 aseptic conditions.

1

0.1 µm Prefiltration I

Removes large aggregates and debris; protects the first 0.04 µm final filter cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a 0.22 µm filter. Filtration is a risk-mitigation step and is not a substitute for lot-specific mycoplasma testing.
3

0.1 µm Prefiltration II

Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter; ISO Class 5 aseptic fill & finish. Final product QC release gate.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size across four filtration passes

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm and 0.04 µm mycoplasma-retentive filtration; this is not a per-lot mycoplasma assay result.

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS DCP-RPMIGH-P1X RPMI 1640 High Glucose 25mM HEPES Quadruple-stage filtration 0.1 micron x2 plus 0.04 micron x2 organ-on-a-chip microfluidic cell culture media Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) — DCP-RPMIGH-P1X.
© Diagnocine® — DCP-RPMIGH-P1X

Applications

OoC and MPS Applications

FluxMPS™ DCP-RPMIGH-P1X delivers Microfluidics Suitable purity for organ-on-a-chip and microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant of this formulation is available on request for automated bioreactors and robotic perfusion systems requiring the ultra nano-filtered tier.

Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates that foul automated systems
Valve & Sensor Protection: Reduces micro-fouling in automated perfusion and recirculation systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

0.04 µm filtered media for organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip platforms where reduced particulate media helps prevent channel fouling.

OoCToCBoCLoCMPS

Cancer Biology

Cancer Cell Lines & Metabolic Research

High-glucose RPMI base supports NCI-60 cancer lines and Warburg-effect metabolic studies with precise carbon-source control.

MCF-7MDA-MB-231HeLaJurkat

Stem Cell Biology

iPSC-Derived Models

Ultra-filtered base for iPSC differentiation protocols requiring defined, low-particulate media.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cells

Low-particulate formulation for primary cells and endothelial monolayer studies on-chip.

HUVECsHAECsPrimary hepatocytes

Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation for ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomics

Live-Cell Imaging

Microscopy & Optical Sensing

Reduced particulate background supports confocal microscopy, biosensor measurements, and TEER monitoring on-chip.

ConfocalBiosensorsTEER

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Certificate of Analysis available: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose, [-] Sodium Pyruvate
Appearance	Red-colored, clear solution
Total ingredients	40
pH USP <791>	7.4
Osmolality USP <785>	Contact for specification
Glucose	4500.000 mg/L
L-Glutamine	300.000 mg/L
Sodium Pyruvate	Not added
Phenol Red	5.300 mg/L (phenol red sodium salt)

Sterility, Purity & Safety Parameters

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL (batch release specification)
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std. ISO	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	5% CO₂ recommended (consistent with 2000 mg/L sodium bicarbonate at pH 7.4); the 25 mM HEPES buffer adds pH stability during open-bench handling

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid: 40 ingredients verified per lot with CAS numbers for full raw-material traceability. All ingredients from the original formulation are preserved exactly.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate	13477-34-4	100.000
Magnesium sulfate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	2000.000
Sodium chloride	7647-14-5	6000.000
Sodium phosphate dibasic anhydrous	7558-79-4	800.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	10.000
L-Arginine hydrochloride	1119-34-2	241.000
L-Asparagine	70-47-3	50.000
L-Aspartic acid	56-84-8	20.000
L-Cystine dihydrochloride	30925-07-6	65.200
L-Glutamic acid	56-86-0	20.000
L-Glutamine	56-85-9	300.000
L-Histidine hydrochloride monohydrate	5934-29-2	20.960
L-Hydroxyproline	51-35-4	20.000
L-Isoleucine	73-32-5	50.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	40.000
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	15.000
L-Proline	147-85-3	20.000
L-Serine	56-45-1	30.000
L-Threonine	72-19-5	20.000
L-Tryptophan	73-22-3	5.000
L-Tyrosine Disodium Salt	69847-45-6	28.830
L-Valine	72-18-4	20.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	3.000
D-Biotin	58-85-5	0.200
D-Ca-Pantothenate	137-08-6	0.250
Folic acid	59-30-3	1.000
Niacinamide	98-92-0	1.000
Pyridoxine hydrochloride	58-56-0	1.000
Riboflavin	83-88-5	0.200
Thiamine hydrochloride	67-03-8	1.000
p-Amino benzoic acid (PABA)	150-13-0	1.000
OTHERS
D-Glucose	50-99-7	4500.000
Glutathione reduced	70-18-8	1.000
HEPES	7365-45-9	5958.000
Phenol red sodium salt	34487-61-1	5.300
i-Inositol	87-89-8	35.000

Customization available: pH, glucose, HEPES, salts, and nutrient composition modifications available on request. Contact support@diagnocine.com.

Quality Assurance

ISO 13485 Manufacturing & Compliance

Every batch of FluxMPS™ DCP-RPMIGH-P1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

verified

ISO 13485:2016 QMS

Full quality management system with documented procedures, deviation control, and CAPA. Every lot traceable from raw material to final release.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity Type 1 water, produced in-house with controlled trace-metal and TOC content.

biotech

ISO Class 5 Fill & Finish

Final aseptic filling in ISO Class 5 (Class 100) cleanroom. Immediate post-filtration fill to reduce recontamination risk.

assignment

Micro-Batch Precision

Small-batch manufacturing with per-lot QC release. Every batch tested independently — not pooled or blended across lots.

Endotoxin USP <85> BET

LAL-based Bacterial Endotoxins Test performed per manufacturing batch. Release specification: < 0.05 EU/mL.

Particulate USP <788> Method 1

Light obscuration particulate analysis. NMT 25/mL at ≥10 µm; NMT 3/mL at ≥25 µm. Consistent with USP <788> parenteral-grade particulate limits.

Osmolality USP <785>

Osmolality verified per lot using vapor pressure or freezing-point depression osmometry per USP <785>.

Documentation / CoA

Full Certificate of Analysis available per lot. Includes all QC parameters, test dates, and raw material lot numbers. Request: support@diagnocine.com.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request at support@diagnocine.com.

Product Comparison

How DCP-RPMIGH-P1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Parameter	DCP-RPMIGH-P1X (FluxMPS™)	Conventional 0.22 µm Filtered	Standard Alternative
Grade	Microfluidics Suitable (0.04 µm)	Not tiered (single-stage filtration)	Not tiered (single-stage filtration)
Formulation	[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 25mM HEPES, [-] Sodium Pyruvate	Standard RPMI 1640	Standard RPMI 1640
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 stages	1 stage	1–2 stages
Mycoplasma-retentive filtration	check_circle	cancel	cancel
Endotoxin (release specification)	FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance	check_circle	cancel	cancel
Water quality	Type 1, 18.2 MΩ·cm	Type 2 typical	Type 2 typical
Manufacturing QMS	ISO 13485:2016	ISO 9001 typical	Variable
Microfluidic channel compatibility	check_circle	cancel	cancel
Custom formulation	check_circle	cancel	Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIGH-P1X and its use in OoC and MPS applications.

Is DCP-RPMIGH-P1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-RPMIGH-P1X is a Microfluidics Suitable formulation engineered for MPS and OoC platforms. The 0.04 µm final filtration reduces the particulate load that can foul microfluidic channels, making it suitable for OoC, ToC, BoC, and LoC applications sensitive to particulate contamination.

What makes the filtration process superior to standard 0.22 µm filtered media?expand_more

Why is sodium pyruvate excluded, and how do I supplement for my cell type?expand_more

This formulation is manufactured without sodium pyruvate so researchers can control carbon-source inputs independently of the 4500 mg/L glucose already present. If your protocol requires pyruvate, add sodium pyruvate (typically 100–110 mg/L final) directly to the medium at time of use; filter any stock solution per the guidance in the next question.

Does this medium require a CO₂ incubator?expand_more

This formulation contains 2000 mg/L (23.8 mM) sodium bicarbonate as its primary buffer, plus 25 mM HEPES as a secondary buffer. The bicarbonate system is calibrated for approximately 5% CO₂ to maintain pH 7.4; incubate at 37°C, 5% CO₂. The added HEPES provides extra pH stability during open-bench handling outside the incubator.

Can DCP-RPMIGH-P1X be supplemented with serum, growth factors, or other components?expand_more

Yes. This is a basal medium formulation. Supplement with FBS, serum replacements, growth factors, or other additives as required by your cell type and experimental protocol. When filtering serum or protein-containing supplements before addition, use a 0.2 µm low-protein-binding PES or PVDF filter; a 0.04 µm membrane is not appropriate for serum and will strip essential lipoproteins and clog rapidly. Defined, protein-free additions may be filtered at 0.1 µm.

What endotoxin level should I expect, and how is it verified?expand_more

Endotoxin is controlled per manufacturing batch to a release specification of < 0.05 EU/mL, verified by the Limulus Amebocyte Lysate (LAL) assay per USP <85> (Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) before each batch is released.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. A CoA is available per lot upon request at support@diagnocine.com. It includes pH, osmolality (where applicable), endotoxin, sterility, mycoplasma filtration statement, USP <788> particulate data, appearance, and full raw material lot traceability.

Scientific References

Supporting literature

Curated peer-reviewed references relevant to OoC/MPS applications and FluxMPS™ ultra-filtered cell culture media.

Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nature Biotechnology. 2014;32(8):760–772.doi:10.1038/nbt.2989
Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507(7491):181–189.doi:10.1038/nature13118
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
Luni C, Serena E, Elvassore N. Human-on-chip for therapy development and fundamental science. Current Opinion in Biotechnology. 2014;25:45–50.doi:10.1016/j.copbio.2013.08.015
Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Pyruvate: 1X Liquid

Engineered where standard media fails

Microchannel-safe purity

Total metabolic control

Ultrapure-grade water

Low background for imaging

Rich, stable nutrient profile

Customization on demand

Quadruple-stage filtration system

0.1 µm Prefiltration I

0.04 µm Final filtration I

0.1 µm Prefiltration II

0.04 µm Final filtration II — Polish

Performance vs. conventional media

OoC and MPS Applications

Automated Bioreactors & Robotics

Micro Physiological System (MPS) & Chip

Cancer Cell Lines & Metabolic Research

iPSC-Derived Models

Endothelial & Primary Cells

Metabolic Flux Analysis

Microscopy & Optical Sensing

Analytical release specifications

Full composition (mg/L)

ISO 13485 Manufacturing & Compliance

ISO 13485:2016 QMS

Ultrapure Type 1 Water

ISO Class 5 Fill & Finish

Micro-Batch Precision

Endotoxin USP <85> BET

Particulate USP <788> Method 1

Osmolality USP <785>

Documentation / CoA

How DCP-RPMIGH-P1X compares

Frequently asked questions

Supporting literature

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