Q Sepharose XL, 300 mL
Cat. No.: GOV-DC-17507201
Pack Size: 300 mL
Feature:
Q Sepharose XL is a strong anion exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
Description:
Q Sepharose XL is a strong anion exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
- High loading capacities give more productive capture from clarified feedstocks.
- Strong anion exchanger designed for capture steps
- High chemical stability for effective CIP/sanitization
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow resin. Dextran chains are covalently coupled to the agarose matrix and modified with quaternary ammonium (Q) strong anion exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.
With its high loading capacities and excellent physical and chemical stability, Q Sepharose XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.
As member of the BioProcess resins range, Q Sepharose XL meets industrial demands with security of supply and comprehensive technical and regulatory support.
| Parameter |
Q Sepharose XL, 300 mL |
| BioProcess resin |
Yes |
| Certificate of Analysis |
Yes |
| Chemical stability |
Commonly used buffers, nonionic detergents, 1 M NaOH, 6 M guanidine hydrochloride |
| Ion Exchanger Type |
Strong anion exchanger |
| Ionic Capacity |
0.18-0.26 mmol Cl-/ml medium |
| Licensing |
For licensing information, see the Licensing Statements page in the About Us section. |
| Ligand |
Quaternary amine |
| Ligand Coupling Method |
Dextran extenders through ether bonds |
| Matrix |
6% cross-linked agarose with dextran surface extenders |
| Particle Size |
45 μm-165 μm |
| Particle size, d50V1 |
~90 μm |
| Pressure/Flow Specification |
300-500 cm/h |
| Storage |
4 to 30°C, 20% Ethanol |
| pH stability, CIP2 |
2-14 |
| pH stability, operational3 |
2-12 |
| Pack size |
300 mL |
- 1Median particle size of the cumulative volume distribution.
- 2pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
- 3pH range where resin can be operated without significant change in function.
