Protease Inhibitor Cocktail with Tris Tween 20 Buffer
Cat. No. DCP-PICTT201X
Size: 10x10 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
This Ready-to-use combination of a Protease Inhibitor Cocktail with Tris-Tween 20 buffer creates a powerful solution for preserving protein integrity in various biochemical applications. The Tris component maintains a stable pH, while Tween 20 aids in cell lysis and protein solubilization. This combination is particularly effective for applications such as Western blotting, immunoprecipitation, and various enzymatic assays, where maintaining native protein structure and activity is crucial. By preventing proteolytic degradation, this buffer system enhances the reliability and reproducibility of experimental results, making it an invaluable tool in protein research and characterization studies.
The cocktail contains AEBSF.HCl, a serine protease inhibitor; Aprotinin, which targets serine proteases and has anti-inflammatory properties; Bestatin, an aminopeptidase inhibitor; E-64, a cysteine protease inhibitor; EDTA, a metalloproteinase inhibitor; Leupeptin, which inhibits both serine and cysteine proteases; and Pepstatin A, an aspartic protease inhibitor. This cocktail effectively minimizes protein degradation in a wide range of experimental procedures, including cell lysis, protein purification, and enzymatic assays. The carefully balanced concentrations of each component ensure optimal inhibition of proteolytic activity while maintaining compatibility with most downstream applications, making it an invaluable tool for researchers working with sensitive protein samples.
Advantages:
- Broad-spectrum inhibition: Protease inhibitor cocktails contain multiple inhibitors that target different classes of proteases, providing comprehensive protection against protein degradation.
- Preservation of protein integrity: The cocktail helps maintain the structural and functional integrity of proteins during extraction, purification, and analysis.
- Reduced non-specific binding: Tween 20 in the buffer decreases non-specific protein-protein interactions and background noise in immunoassays.
- Improved washing efficiency: TBST (Tris-buffered saline with Tween 20) is effective for washing steps in Western blotting and other immunoassays.
Application:
- Cell lysis: To extract proteins while preventing their degradation.
- Western blotting: During membrane washing steps to reduce background while protecting proteins.
- Immunoprecipitation: To maintain protein integrity during binding and washing steps.
Composition:
| Ingredients |
Concentration |
| AEBSF.HCl |
100 uM |
| Aprotinin |
0.8 uM |
| Bestatin |
0.05 mM |
| E-64 |
1 uM |
| EDTA |
5 mM |
| Leupeptin |
0.01 mM |
| Pepstatin A |
1 uM |
| Tween 20 |
0.1% |
| Tris-HCl |
50 mM |
| Sodium Chloride |
150 mM |
Quality Control:
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Appearance: Clear solution.
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -80 ℃
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Shelf life: 3 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
