DiagnoCine Precision® ELISA Kit Reagents included
- SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
- Universal Blocking Buffer: use when background interference compromises signal-to-noise ratio
- Protease Inhibitor Cocktail: incorporate during the sample preparation phase or add directly to the ELISA protocol as required to prevent protein degradation.
Exclusive additions: All kits come with three DiagnoCine Precision® reagents for improving the effectiveness of your testing and clarity of your results
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Pig Dehydroepiandrosterone (DHEA)
Cat.No.: JNB-JL26061
Size: 96TSpecies: PigAssay Type: CompetitiveSensitivity: 0.071ng/mLDetection Range: 0.156-10ng/mLAlternative Names: 3β-Hydroxyandrost-5-en-17-one;Androstenolone;Prasterone;Androst-5-en-3β-ol-17-one; 5,6-Didehydroepiandrosterone;DehydroisoepiandrosteroneResearch Areas: Cardiovascular BiologyAssay Time: 1h15mAbbreviation: DHEATest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with Pig DHEA antibody. Add Sample, Standard, and HRP-antigen by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is opposite to the Pig DHEA in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. You can calculate the concentration of Pig DHEA in the samples by comparing the OD of the samples to the standard curve.Specificity: This kit recognizes Pig DHEA in samples. No significant cross-reactivity or interference between Pig DHEA and analogues was observed.Recovery rate: 80%-120%Precision: Intra-CV and Inter-CV <10% Shipping: Ice Bag