UltraClean PVSA-TAE, 1X
Cat No.:
DCP-TAEPVSA1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest RNase-safe buffer for RNA handling, cell, and molecular biology experiments
Description:
PVSA-TAE, 1X is a ready-to-use, high-purity Tris–acetate–EDTA buffer formulated to be RNase-safe for RNA and DNA workflows that use TAE as the primary buffer system. It is supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor that helps preserve RNA integrity during sample handling, electrophoresis preparation, and short-term storage. The buffer provides the ionic strength and buffering characteristics of standard 1X TAE, making it suitable for use with nucleic acid samples, gels, and electrophoresis-related procedures where RNA and DNA stability are critical.
Applications:
- RNA and DNA Sample Preparation: Dilution and preparation of RNA and DNA samples in a TAE-based buffer with added RNase protection prior to loading on gels or downstream processing.
- Electrophoresis-Related Workflows: Buffer environment for nucleic acid handling in workflows associated with agarose gel electrophoresis when RNA integrity must be maintained.
- RNA Handling and Short-Term Storage: Temporary storage of RNA-containing samples, purified nucleic acids, or gel-extracted fragments in an RNase-controlled TAE buffer.
- Probe and Fragment Handling: Dilution and handling of oligonucleotides, probes, and nucleic acid fragments in workflows that are standardized around TAE rather than other buffer systems.
- General Molecular Biology: Any procedure requiring 1X TAE with enhanced RNA protection, where compatibility with TAE-based protocols is desired.
Advantages:
- RNase-Safe: PVSA provides robust inhibition of a wide range of RNases, enhancing RNA protection without relying solely on protein-based inhibitors.
- High Purity: Prepared from molecular biology-grade Tris, EDTA, and PVSA, and filtered to reduce particulates, microbes, and adventitious nucleases.
- Nucleic Acid Friendly: Low ionic strength and EDTA chelation support nucleic acid stability while limiting divalent-cation–dependent nuclease activity.
- Versatile Buffer System: TE-based formulation is compatible with many standard nucleic acid handling and storage protocols.
- Ready-to-Use 1X Solution: Supplied at working strength to reduce preparation time and contamination risk.
Composition:
| Ingredients |
Concentration |
| Tris base |
40 mM |
| Acetate |
20 mM |
| EDTA |
1 mM |
| PVSA (Polyvinylsulfonic Acid) |
1g/L |
| Water (Ultrapure, nuclease-free) |
To volume |
Quality Control:
Storage and Shelf Life:
-
Store at 2–8°C.
-
Do not freeze.
-
Shelf life: 12 months.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
