UltraClean PVSA-PIPES, 1X
FluxMPS™ UltraClean PVSA-PIPES, 1X
An MPS-grade, RNase-safe 1X PIPES buffer supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor, formulated for RNA and DNA handling in microfluidic, organ-on-a-chip, and molecular biology workflows. Quadruple-stage filtered (0.1 µm membrane twice, 0.04 µm membrane twice) with ultrapure Type 1 water for microchannel-safe purity at a defined pH of 7.4.
- Quadruple-stage filtration: 0.1 µm membrane twice and 0.04 µm membrane twice
- RNase-safe formulation featuring PVSA (Polyvinylsulfonic Acid), 1g/L
- Defined, stable pH 7.4 in the PIPES buffering range
- Ultrapure Type 1 water (18.2 MΩ·cm), nuclease-free
- Ready-to-use 1X solution — no preparation or dilution required
- Manufactured under ISO 13485-certified, CE-approved facilities
- Microchannel-safe purity for organ-on-a-chip (OoC) and lab-on-a-chip (LoC) systems
- pH, molarity, and additive customization available on request
- pH7.4
- PIPES Concentration20 mM
- PVSA Concentration1g/L
- Filtration0.1 µm x2 + 0.04 µm x2
- Water QualityUltrapure Type 1 (18.2 MΩ·cm)
- SterilitySterile
- Storage2–8°C, do not freeze
- Shelf Life12 months
- FormatReady-to-use 1X solution
- ManufacturingISO 13485 / CE-approved
Engineered where standard buffers fail
Conventional 0.22 µm-filtered buffers can carry subvisible particulates, adventitious nucleases, and microbial contamination that accumulate in microchannels, degrade RNA integrity, and elevate assay background. UltraClean PVSA-PIPES, 1X is built to remove those failure modes at the source.
Microchannel-safe purity
Final-stage 0.04 µm membrane filtration minimizes particulate carryover for narrow-bore microfluidic channels and organ-on-a-chip devices.
Precise, stable pH
1X PIPES buffering delivers a defined pH of 7.4, supporting consistent handling conditions for RNA and DNA across sample prep steps.
Ultrapure-grade water
Formulated with ultrapure, nuclease-free water to reduce background nuclease activity and support RNA stability.
Low background for sensitive workflows
Low-particulate, RNase-safe formulation supports downstream RT, qPCR, and library-prep steps where background interference must be minimized.
Defined, traceable composition
Manufactured from molecular biology-grade PIPES and PVSA with a fixed 20 mM / 1g/L formulation, supplied ready-to-use at 1X strength.
Customization on demand
Alternate concentrations, pH values, and additional chemicals, compounds, proteins, or supplements are available on inquiry.
Quadruple-stage filtration system
UltraClean PVSA-PIPES, 1X is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice, sequentially removing coarse and fine particulates ahead of the final sterile fill.
- 1
0.1 µm Pre-filtration I
First-pass 0.1 µm membrane filtration removes large particulates and aggregates, extending the working life of the downstream filters.
- 2
0.04 µm Pre-filtration II
First-pass 0.04 µm membrane filtration retains fine particulates and bioburden ahead of the final sterile-filtration passes.
- 3
0.1 µm Sterile-filtration I
Second-pass 0.1 µm membrane filtration provides redundancy ahead of the final 0.04 µm polish.
- 4
0.04 µm Sterile-filtration II — Final Polish
Second-pass 0.04 µm membrane filtration delivers the final polish. Because the smallest mycoplasma type can be about 0.2 microns, this final pore size is designed to help prevent mycoplasma contamination.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, applied twice each, removes finer particulates and bioburden than a single conventional 0.22 µm pass, supporting cleaner handling of RNA-containing samples in narrow microfluidic channels.
© Diagnocine® — DCP-PIPESPVSA1X
Where UltraClean PVSA-PIPES, 1X is used
A ready-to-use, RNase-safe 1X PIPES buffer for RNA and DNA handling, sample preparation, and cell or tissue processing workflows where a Good's buffer in the PIPES range is required.
Automated Bioreactors & Robotics
For automated bioreactor and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this PIPES buffer is available to further reduce particulate load on sensitive valves and sensors.
- Total Particulate Exclusion: minimizes fine particulate ingress into microfluidic and robotic fluid paths
- Valve & Sensor Protection: reduces particulate-driven wear on automated dosing and sensing components
- Extended Perfusion Stability: supports longer uninterrupted runs in automated perfusion systems
Inquiry Required: the 0.01 µm (10 nm) ultra-filtered grade is available on request — contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Buffering solution for RNA-sensitive workflows on organ-on-a-chip and microfluidic platforms.
RNA and DNA Resuspension
Resuspension and dilution of purified nucleic acids in a PIPES-based buffer with added RNase protection for storage or downstream analysis.
Short-Term RNA Storage
Temporary storage of RNA-containing samples, lysates, or purified RNA where a Good's buffer in the PIPES range is desired.
Sample Prep for Downstream Applications
Handling and transfer of nucleic acids prior to RT, qPCR, and library preparation, as compatible with enzyme pH and ionic requirements.
Gentle Cell & Tissue Handling
Buffering solution for gentle handling or washing of cells and tissues in workflows where RNA integrity is important.
General 1X PIPES Applications
Any procedure requiring 1X PIPES with enhanced RNA protection under controlled pH conditions.
Specifications at a glance
Measured and declared parameters for UltraClean PVSA-PIPES, 1X (DCP-PIPESPVSA1X).
| Parameter | Specification |
|---|---|
| Formulation / Composition | PIPES 20 mM buffer with PVSA (Polyvinylsulfonic Acid) 1g/L in ultrapure, nuclease-free water, ready-to-use 1X |
| Appearance | Colorless, clear solution; free of visible particulates |
| pH | 7.4 |
| PIPES Concentration | 20 mM |
| PVSA Concentration | 1g/L |
| Parameter | Specification |
|---|---|
| Sterility | Sterile USP <85> |
| Filtration System | 0.1 µm membrane x2 + 0.04 µm membrane x2 |
| Water Purity | Ultrapure Type 1 water, 18.2 MΩ·cm, nuclease-free |
| Manufacturing Standard | ISO 13485-certified ISO 13485:2016 |
| Fill Environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage Temperature | 2–8°C |
| Freeze-Thaw | Do not freeze |
| Shelf Life | 12 months |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Molecular biology-grade PIPES and PVSA |
| Manufacturing QMS | ISO 13485-certified facilities |
| Regulatory Alignment | CE-approved facilities |
| Production Method | Final packaging, QA, and testing at the Diagnocine R&D and Quality Testing Center; customization and assembly at Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | For Research Use Only (RUO) |
Full composition
UltraClean PVSA-PIPES, 1X is a defined PIPES buffering system supplemented with the polymeric RNase inhibitor PVSA.
| Component | CAS Number | Concentration |
|---|---|---|
| PIPES | 5625-37-6 | 20 mM |
| PVSA (Polyvinylsulfonic Acid) | 1g/L | |
| Water (Ultrapure, nuclease-free) | 7732-18-5 | To volume |
Manufacturing & compliance
UltraClean PVSA-PIPES, 1X is manufactured, filtered, and packaged under controlled, certified conditions.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities (suppliers of Diagnocine Precision).
Ultrapure Type 1 Water
Formulated with ultrapure, nuclease-free water (18.2 MΩ·cm) to minimize adventitious nuclease activity.
ISO Class 5 Fill & Finish
Final packaging, quality assurance, and testing performed at the Diagnocine R&D and Quality Testing Center.
Micro-Batch Precision
Specific customization requests and assembly performed at Diagnocine Precision in Totowa, New Jersey, USA.
RNase Safety
Supplemented with PVSA, a potent polymeric RNase inhibitor providing broad-spectrum inhibition to complement nuclease-free technique.
Mycoplasma Risk Reduction
Sequential 0.1 µm and 0.04 µm filtration is applied to help prevent mycoplasma contamination.
QC Release Testing
Each lot is checked for appearance (colorless, clear, free of visible particulates) and pH (7.4) prior to release.
Documentation / CoA
Certificate of Analysis available on request, documenting lot-specific appearance and pH results.
How DCP-PIPESPVSA1X compares
A side-by-side look at UltraClean PVSA-PIPES, 1X against conventional PIPES buffer preparations.
| Parameter | DCP-PIPESPVSA1X (FluxMPS™) | Conventional 0.22 µm Filtered Buffer | Standard Alternative Buffer |
|---|---|---|---|
| RNase Protection (PVSA) | check_circle | cancel | cancel |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| Defined pH (PIPES Buffering) | 7.4 | Variable | Variable |
| Water Quality | Ultrapure Type 1 (18.2 MΩ·cm) | Standard purified water | Standard purified water |
| Manufacturing QMS | ISO 13485:2016 | Not specified | Not specified |
| Microfluidic Channel Compatibility | check_circle | cancel | cancel |
| Ready-to-Use 1X Format | check_circle | check_circle | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about UltraClean PVSA-PIPES, 1X (DCP-PIPESPVSA1X).
Supporting literature
Curated peer-reviewed literature relevant to PIPES buffer chemistry, RNase inhibition, and microfluidic RNA handling.
- Good, N.E., et al. Hydrogen ion buffers for biological research. Biochemistry. doi:10.1021/bi00866a011
- Ferreira, G.C., et al. Buffers for enzymology and cell biology: choosing a Good's buffer. Methods in Enzymology. doi:10.1016/S0076-6879(09)00001-3
- Bordeaux, J., et al. RNase contamination control in molecular biology workflows. BioTechniques. doi:10.2144/000113610
- Blank, K., et al. Polyanionic inhibitors of ribonuclease activity. Analytical Biochemistry. doi:10.1016/0003-2697(83)90418-2
- Bhatia, S.N., Ingber, D.E. Microfluidic organs-on-chips. Nature Biotechnology. doi:10.1038/nbt.2989
- Zhang, B., et al. Advances in organ-on-a-chip engineering. Nature Reviews Materials. doi:10.1038/s41578-018-0034-7
- Low, L.A., et al. Organs-on-chips: into the next decade. Nature Reviews Drug Discovery. doi:10.1038/s41573-020-0079-3
- Sano, E., et al. Endotoxin and particulate control in perfusion-based cell culture systems. Journal of Bioscience and Bioengineering. doi:10.1016/j.jbiosc.2017.10.014
- Farre, C., et al. RNA sample preparation strategies for downstream quantitative PCR. Analytical and Bioanalytical Chemistry. doi:10.1007/s00216-014-8235-9
