UltraClean PVSA-Supplemented PBS Buffer, 1X (Phosphate Buffered Saline), RNase-Safe
Cat No.:
DCP-PBSPVSA1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest RNase-safe PBS buffer for RNA handling, cell, and molecular biology experiments
Description:
PVSA-Supplemented PBS Buffer, 1X is a ready-to-use, high-purity phosphate-buffered saline formulated to be RNase-safe for demanding molecular biology and cell biology workflows. It is supplemented with polyvinylsulfonic acid (PVSA), a potent, polymeric RNase inhibitor that helps preserve RNA integrity during sample handling, washing, and storage. The buffer is isotonic and maintained near physiological pH, making it suitable for use with cells, tissues, and RNA-containing preparations in sensitive downstream applications.
Applications:
-
RNA Handling and Storage: Washing, resuspension, and temporary storage of RNA-containing samples, cells, or tissues where RNase control is critical.
-
RNA Extraction and Purification: RNase-safe wash and resuspension buffer for columns, magnetic beads, or cell pellets in RNA isolation workflows.
-
RNA FISH / In Situ Hybridization: Preparation and washing of samples and probes in RNA fluorescence in situ hybridization and related hybridization assays.
-
RT-qPCR and RNA Library Prep: Dilution of RNA samples, standards, and primers, and washing of reaction components in workflows requiring strict nuclease control.
-
General Molecular Biology: Any procedure requiring isotonic PBS with enhanced RNA protection, including immunostaining steps on RNA-sensitive samples and gentle cell handling.
Advantages:
-
RNase-Safe: PVSA provides robust inhibition of a wide range of RNases, protecting RNA without relying solely on protein-based inhibitors.
-
High Purity: Manufactured from molecular biology-grade components and subjected to multiple fine-pore filtration steps to minimize particulates, microbes, and adventitious nucleases.
-
Isotonic and Physiological pH: Formulated to maintain osmolarity and pH compatible with mammalian cells and tissues, reducing cellular stress during handling.
-
Free of Added Calcium and Magnesium: Lacks divalent cations that can activate certain nucleases or affect cell adhesion and signaling, improving compatibility with many assays.
-
Ready-to-Use 1X Solution: No dilution or mixing required, minimizing preparation time and contamination risk.
Composition:
| Ingredients |
Concentration |
| Sodium Chloride (NaCl) |
137 mM |
| Potassium Chloride (KCl) |
2.7 mM |
| Sodium Phosphate Dibasic (Na₂HPO₄) |
10.1 mM |
| Potassium Phosphate Monobasic (KH₂PO₄) |
1.76 mM |
| PVSA (Polyvinylsulfonic Acid) |
1 g/L |
| Water (Ultrapure, nuclease-free) |
To volume |
Quality Control:
Storage and Shelf Life:
-
Store at 2–8°C.
-
Do not freeze.
-
Shelf life: 12 months.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
