UltraClean PVSA-Citrate, 1X
Cat No.:
DCP-CITRAPVSA1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest RNase-safe buffer for RNA handling, cell, and molecular biology experiments
Description:
PVSA-Citrate, 1X is a ready-to-use, high-purity sodium citrate buffer formulated to be RNase-safe for sensitive RNA and DNA workflows in the mildly acidic to near-neutral pH range. It is supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor that helps preserve RNA integrity during sample handling, processing, and short-term storage. The buffer provides defined citrate-based buffering and ionic strength, making it suitable for use with purified nucleic acids, RNA-containing samples, and cells in downstream molecular biology applications where citrate is preferred as the buffering system.
Applications:
- RNA and DNA Resuspension: Resuspension and dilution of nucleic acids in a citrate-based buffer with added RNase protection for storage or downstream analysis.
- RNA Handling and Short-Term Storage: Temporary storage of RNA-containing samples, lysates, or purified RNA in a controlled, low-to-moderate ionic strength citrate buffer.
- Sample Preparation for Enzymatic Assays: Handling and transfer of nucleic acids prior to downstream applications (e.g., RT, qPCR, library prep), as compatible with enzyme-specific pH and salt requirements.
- Cell and Tissue Processing: Gentle washing or processing of cells and tissues in workflows where RNA integrity is important and citrate buffer is desired.
- General Molecular Biology: Any procedure requiring 1X citrate buffer with enhanced RNA protection under defined pH and ionic conditions.
Advantages:
- RNase-Safe: PVSA provides robust inhibition of a broad spectrum of RNases, adding an extra layer of protection beyond standard nuclease?free practices.
- High Purity: Manufactured from molecular biology–grade sodium citrate and PVSA and finely filtered to minimize particulates, microbial contamination, and adventitious nucleases.
- Defined Buffering Environment: Citrate buffering supports stable pH in the selected range while providing gentle ionic conditions for nucleic acids.
- Versatile Usage: Suitable for workflows that prefer phosphate?free, citrate?based buffers or require reduced divalent cation availability.
- Ready-to-Use 1X Solution: Supplied at working strength to reduce preparation time, variability, and contamination risk.
Composition:
| Ingredients |
Concentration |
| Sodium Citrate |
10 mM |
| PVSA (Polyvinylsulfonic Acid) |
1g/L |
| Water (Ultrapure, nuclease-free) |
To volume |
Quality Control:
Storage and Shelf Life:
-
Store at 2–8°C.
-
Do not freeze.
-
Shelf life: 12 months.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
