Sterile; Ultrapure, Filtered 0.1-micron membrane Once
World’s cleanest buffer for all cell and molecular biology experiments
Description
TBS Blocking Buffer with PVP(Polyvinylpyrrolidone) is used in various immunoassays, such as Western blotting and ELISA, to reduce non-specific binding and improve assay sensitivity. PVP is a synthetic polymer, which means it does not contain proteins that could potentially interfere with the detection of protein targets. TBS can help reduce nonspecific background staining, which is particularly beneficial in applications like immunohistochemistry (IHC) where background noise can obscure results. This is especially important in tissues with high endogenous peroxidase activity. TBS is preferred over PBS when using alkaline phosphatase-conjugated detection systems. PBS contains phosphate, which can inhibit the activity of alkaline phosphatase, leading to reduced signal intensity in assays. TBS has a higher buffering capacity compared to PBS, which can be advantageous in maintaining a stable pH during longer incubation periods or under varying temperature conditions.
Applications
Western Blotting: PVP in TBS is used to block non-specific binding sites on membranes, ensuring that antibodies bind specifically to target proteins. This helps in achieving clearer and more specific detection of target proteins.
ELISA: In enzyme-linked immunosorbent assays, PVP can be used to block non-specific sites on microplates, improving the accuracy and sensitivity of the assay by reducing background noise.
Detection of Small Proteins: PVP is particularly useful for detecting small proteins, as it does not contain proteins that might interfere with the assay, unlike protein-based blockers such as milk or BSA.
Advantages:
Non-Protein Blocking Agent: Being a non-protein polymer, PVP does not introduce additional proteins into the assay system, which could potentially interfere with the detection of target proteins. This makes it ideal for assays where protein-protein interactions are a concern.
Low Autofluorescence: PVP exhibits very low autofluorescence, making it suitable for assays involving fluorescence detection, as it minimizes background noise in detection channels.
Rapid Blocking: PVP-40 has been shown to provide effective blocking in a shorter time compared to traditional blockers like skim milk. For example, PVP-40 blocking for 10 minutes can achieve similar results to 1-hour skim milk blocking.
Compatibility: PVP is compatible with various detection systems and can be used in combination with other blocking agents like casein to enhance blocking efficiency. It is also suitable for use with nitrocellulose and PVDF membranes.
Versatility: TBS is often preferred over PBS in blocking buffers when alkaline phosphatase is used as a detection enzyme, as PBS can interfere with its activity.
Composition:
Ingredients
Concentration
PVP-40
1%
Tris-Base
50 mM
Sodium Chloride
150 mM
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.5
Sterility: Filtered 0.1 micron Once in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: 4 ℃
Shelf life: 9 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
DiagnoCine Precision Sterile buffers can be filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration two times. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
