PBS Blocking Buffer with PVP
FluxMPS™ PBS Blocking Buffer with PVP
An MPS-grade, sterile-filtered phosphate-buffered saline formulated with 1% PVP-40, a non-protein blocking polymer engineered for Western blotting, ELISA, and other immunoassays that demand low-autofluorescence, high-specificity blocking. Single-pass 0.1 µm membrane filtration and a precisely defined pH 7.5 formulation deliver ultra-clean, reproducible buffer performance for microfluidic and molecular biology workflows.
- Sterile, single-pass 0.1 µm membrane-filtered buffer
- Formulated with 1% PVP-40, a synthetic non-protein blocking polymer
- Defined pH 7.5 phosphate-buffered saline chemistry (137 mM NaCl, 2.7 mM KCl, 10.1 mM Na2HPO4, 1.76 mM KH2PO4)
- Low autofluorescence formulation minimizes background in fluorescence-based detection
- DNase- and RNase-tested, no activity detected
- Manufactured under ISO 13485-certified, CE-approved facilities
- Custom pH, concentration, and additive formulations available on request
- pH7.5
- FormulationPBS with 1% PVP-40
- Sodium Chloride137 mM
- Potassium Chloride2.7 mM
- Sodium Phosphate Dibasic10.1 mM
- Potassium Phosphate Monobasic1.76 mM
- Filtration0.1 µm membrane, once
- SterilitySterile-filtered
- Storage4°C
- Shelf Life9 months
Engineered where standard blocking buffers fail
Conventional 0.22 µm-filtered buffers and protein-based blockers can introduce subvisible particulates, non-specific protein binding, and background autofluorescence that compromise sensitive immunoassays and imaging-based readouts. FluxMPS™ PBS Blocking Buffer with PVP is formulated and filtered to minimize these failure modes.
Microchannel-safe purity
Single-pass 0.1 µm membrane filtration in a sterile environment removes particulates that can accumulate in fine microfluidic channels and pipette tips.
Precise, stable pH
Formulated at pH 7.5 with a defined phosphate-buffered saline composition for consistent, reproducible blocking performance lot to lot.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm), meeting USP <85> water quality expectations for sensitive assay buffers.
Low background for imaging & assays
PVP-40 exhibits very low autofluorescence, reducing background noise in fluorescence detection channels compared with protein-based blockers.
Defined, traceable composition
Every salt and polymer component is formulated to a stated concentration, supporting reproducible blocking chemistry across experiments.
Customization on demand
Alternate concentrations, additional chemicals, compounds, proteins, supplements, or pH values can be formulated on request.
Single-stage 0.1 µm filtration
FluxMPS™ PBS Blocking Buffer with PVP is sterile-filtered through a 0.1 µm membrane once, in a sterile environment, delivering an ultra-clean buffer for cell and molecular biology experiments.
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0.1 µm Sterile Filtration
The buffer is passed once through a 0.1 µm membrane in a sterile environment, reducing particulates and bioburden for research-grade purity.
Performance vs. conventional buffer
A single-pass 0.1 µm sterile filtration removes finer particulates than a conventional 0.22 µm filtration pass, supporting cleaner blocking and detection chemistry.
© Diagnocine® — DCP-PVPPBS1X
Where FluxMPS™ PBS Blocking Buffer with PVP is used
A non-protein blocking buffer suited to immunoassays, microfluidic sample handling, and imaging workflows where background reduction and reproducible chemistry matter.
Automated Bioreactors & Robotics
For automated liquid handling, robotics, and closed bioreactor systems, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer can be formulated to further protect fine valves and sensors from particulate fouling.
- Total Particulate Exclusion for sensitive automated fluid paths
- Valve & Sensor Protection in closed-loop and robotic systems
- Extended Perfusion Stability for long-duration automated protocols
Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade for automated bioreactor or robotic platforms.
Micro Physiological System (MPS) & Chip
Low-particulate, sterile-filtered buffer suitable for use in microfluidic and chip-based culture and assay systems.
Wash, Dilution & Reconstitution
A defined-pH, sterile PBS base suitable for washing, diluting, and reconstituting samples ahead of downstream assays.
iPSC-Derived Model Handling
Non-protein, low-background buffer compatible with sensitive handling of iPSC-derived cell models.
Endothelial & Primary Cell Perfusion
Sterile, defined-composition PBS suitable for wash and perfusion steps with endothelial and primary cell cultures.
ELISA, Blotting & Blocking
1% PVP-40 blocks non-specific binding on nitrocellulose and PVDF membranes and microplates, improving specificity in Western blotting and ELISA.
Microscopy & Optical Sensing
Very low PVP autofluorescence reduces background in fluorescence detection channels used in confocal and biosensor workflows.
Full specification summary
Measured and declared parameters for FluxMPS™ PBS Blocking Buffer with PVP.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Phosphate-buffered saline with 1% PVP-40 |
| Appearance | Clear, colorless liquid |
| pH USP <791> | 7.5 |
| Sodium Chloride | 137 mM |
| Potassium Chloride | 2.7 mM |
| Sodium Phosphate Dibasic Anhydrous | 10.1 mM |
| Potassium Phosphate Monobasic Anhydrous | 1.76 mM |
| Parameter | Specification |
|---|---|
| Sterility | Filtered 0.1 µm membrane once in a sterile environment |
| DNase Activity | None detected (plasmid DNA, 18 hr incubation, room temperature) |
| RNase Activity | None detected (ribosomal RNA, 18 hr incubation, room temperature) |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facilities |
| Fill Environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 9 months |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO 13485 | ISO 13485:2016 |
| Regulatory Alignment | CE-approved manufacturing facilities |
| Production Location | Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Every buffer salt and blocking polymer component, exactly as formulated in FluxMPS™ PBS Blocking Buffer with PVP.
| Component | CAS Number | Concentration |
|---|---|---|
| PVP-40 (Polyvinylpyrrolidone) | 9003-39-8 | 1% |
| Sodium Chloride (NaCl) | 7647-14-5 | 137 mM |
| Potassium Chloride (KCl) | 7447-40-7 | 2.7 mM |
| Sodium Phosphate Dibasic Anhydrous (Na2HPO4) | 7558-79-4 | 10.1 mM |
| Potassium Phosphate Monobasic Anhydrous (KH2PO4) | 7778-77-0 | 1.76 mM |
Manufacturing & compliance
FluxMPS™ PBS Blocking Buffer with PVP is manufactured, packaged, and tested under a controlled quality system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities that supply Diagnocine Precision.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base.
ISO Class 5 Fill & Finish
Final filtration and fill are performed in an ISO Class 5 (Class 100) controlled environment.
Micro-Batch Precision
Final packaging, quality assurance, and testing are completed at the Diagnocine R&D and Quality Testing Center.
DNase / RNase Testing
Each lot is evaluated for DNase and RNase activity after 18 hr incubation with plasmid DNA and ribosomal RNA, respectively, at room temperature.
Sterility Assurance
Sterile filtration through a 0.1 µm membrane is performed once in a sterile environment.
Formulation Verification
Buffer salt and PVP-40 concentrations are formulated to the stated targets for each production lot.
Documentation / CoA
Certificates of Analysis document appearance, pH, sterility, and nuclease testing results for this product.
How DCP-PVPPBS1X compares
A snapshot of how this PVP-based blocking buffer compares with conventional protein-based and standard blocking buffer options.
| Parameter | DCP-PVPPBS1X (FluxMPS™) | Conventional Protein-Based Blocker | Standard 0.22 µm Buffer |
|---|---|---|---|
| Blocking chemistry | check_circle PVP-40 (non-protein) | cancel Protein-based (milk/BSA) | cancel Protein-based (milk/BSA) |
| Final filtration pore size | 0.1 µm | 0.22 µm | 0.22 µm |
| Sterile filtration | check_circle | check_circle | cancel |
| Low autofluorescence | check_circle | cancel | cancel |
| DNase / RNase tested | check_circle | cancel | cancel |
| ISO 13485 manufacturing | check_circle | cancel | cancel |
| Microfluidic / immunoassay compatible | check_circle | check_circle | check_circle |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about FluxMPS™ PBS Blocking Buffer with PVP.
Supporting literature
Curated literature supporting the use of PVP-based blocking chemistry, phosphate-buffered saline, and sterile-filtered buffers in immunoassays and microfluidic research.
- Bass, J.J. et al. An overview of technical considerations for Western blotting applications to physiological research. Scand J Med Sci Sports. 2017. doi:10.1111/sms.12702
- Mahmood, T. and Yang, P.C. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012. doi:10.4103/1947-2714.100998
- Aydin, S. A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides. 2015. doi:10.1016/j.peptides.2014.11.005
- Kurien, B.T. and Scofield, R.H. Western blotting. Methods. 2006. doi:10.1016/j.ymeth.2005.07.018
- Bewick, V., Cheek, L. and Ball, J. Statistics review: presenting and summarising data. Crit Care. 2003. doi:10.1186/cc2401
- Bhatia, S.N. and Ingber, D.E. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Ronaldson-Bouchard, K. and Vunjak-Novakovic, G. Organs-on-a-chip: a fast track for engineered human tissues in drug development. Cell Stem Cell. 2018. doi:10.1016/j.stem.2018.02.011
- Good, N.E. et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011