Sterile; Ultrapure, Filtered 0.1-micron membrane Once
World’s cleanest buffer for all cell and molecular biology experiments
Description
PBS Blocking Buffer with PVP is used in immunoassays such as Western blotting and ELISA to prevent non-specific binding, thereby enhancing the specificity and sensitivity of the assays. PVP is a synthetic polymer, which means it does not contain proteins that could potentially interfere with the detection of protein targets. This makes it particularly useful for detecting small proteins or when protein-protein interactions are a concern. PVP has very low autofluorescence, making it advantageous for assays involving fluorescence detection. This helps minimize background noise in detection channels, leading to clearer and more reliable results. PVP can provide effective blocking in a shorter time compared to traditional protein-based blockers.
* PBS can interfere with alkaline phosphatase activity, so TBS might be preferred if alkaline phosphatase is used as a detection enzyme.
Applications
Western Blotting: PVP is used as a blocking agent to prevent non-specific binding of antibodies to nitrocellulose and PVDF membranes. This helps in achieving clearer and more specific detection of target proteins.
ELISA: In enzyme-linked immunosorbent assays, PVP can be used to block non-specific sites on microplates, improving the accuracy and sensitivity of the assay by reducing background noise.
Detection of Small Proteins: PVP is particularly useful for detecting small proteins, as it does not contain proteins that might interfere with the assay, unlike protein-based blockers such as milk or BSA.
Advantages:
Low Autofluorescence: PVP has the advantage of no or very-low autofluorescence in detection channels, making it suitable for assays requiring fluorescence detection.
Effective Blocking: PVP, particularly PVP-40, has been shown to provide effective blocking in a shorter time compared to traditional blocking agents like skim milk. For example, PVP-40 blocking for 10 minutes can achieve comparable results to skim milk blocking for 1 hour.
Non-Protein Blocking Agent: As a non-protein polymer, PVP is useful for detecting small proteins and can be combined with other blocking agents like casein to enhance blocking efficiency.
Compatibility: PVP is compatible with nitrocellulose and PVDF membranes and can be used in various buffer systems, including PBS and TBS.
Composition:
Ingredients
Concentration
PVP-40
1%
Sodium Chloride
137 mM
Potassium Chloride
2.7 mM
Sodium Phosphate Dibasic Anhydrous
10.1 mM
Potassium Phosphate Monobasic Anhydrous
1.76 mM
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.5
Sterility: Filtered 0.1 micron once in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: 4 ℃
Shelf life: 9 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
DiagnoCine Precision Sterile buffers can be filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration two times. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
