PNGase F
Cat. No.: FNK-RP802-075
Size: 75000units
Storage: -20℃
Description:
PNGaseF(N-GlycosidaseF) is a recombinant glycosidase from
Elizabethkingia miricola (Flavobacterium Meningosepticum).
Specifications:
Supplied Reagents
PNGaseF
10 X PNGaseF Reaction Buffer
10 X Denaturing Solution
10% NP-40
Concentration : 500,000units/mL
Storage Buffer:
20 mM Tris-HCl (pH7.5 at 25℃)
50 mM NaCl
5 mM EDTA50% Glycerol
10 X PNGaseF Reaction Buffer: 0.5 M Sodium Phosphate(pH7.5)
10 X Denaturing Buffer: 5% SDS, 0.4M DTT
Source: Recombinant protein with a N-terminal DYKDDDDK tag and 6xHis tag, expressed in E.coli.
Molecular Weight: 36kDa.
Purity: > 95%, as determined by SDS-PAGE visualized by CBB stain.
Unit Definition: One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10µg of denatured RNase B in 1hour at 37℃ in a total reaction volume of 10µl.
Standard Procedure:
1. Combine 1-20 μg of glycoprotein, 1 μl of 10X Denaturing Buffer and H20 (if necessary) to make a 10 μl total reaction volume.
2. Denature glycoprotein by heating reaction at 100℃ for 10 minutes.
3. Make a total reaction volume of 20 μl by adding 2 μl of 10X PNGase Reaction Buffer, 2 µl of 10% NP-40, H20 and 1-5 μl PNGaseF.
4. Incubate at 37 ℃ for 1 hour
References:
1) Maley, F. et al. (1989). Anal. Biochem. 180, 195-204.
2) Tretter, V. et al. (1991). Eur. J. Biochem.. 199, 647-652.
3) Plummer, T.H. Jr. and Tarentino, A.L. (1991). Glycobiology. 1, 257-263.
| For Research Use Only. Not for use in diagnostic procedures. |
