PBST with Bovine Serum Albumin (3%) Blocking Buffer
Cat No.: DCP-BSAPBST3X
Size: 500 mL & 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
PBST Blocking Buffer with Bovine Serum Albumin (3%) is a specialized reagent used in various immunoassays, including Western blotting, ELISA, and immunohistochemistry. BSA-PBST blocking buffer is an essential reagent in many laboratory protocols, helping to improve the specificity and sensitivity of protein detection assays while reducing background interference. Bovine Serum Albumin (BSA) acts as the primary blocking agent. It has a strong binding affinity, making it effective in blocking assays. BSA blocking buffer is typically more effective than non-fat milk in systems that utilize biotin-avidin interactions because it contains a single purified protein devoid of endogenous biotin. Phosphate Buffered Saline (PBS) serves as the buffer medium. Tween-20 at 0.05% helps to reduce surface tension and improve the blocking efficiency.
Note: It's important to note that while BSA blocking buffer is highly effective, the optimal blocking agent can vary depending on the specific assay and antibodies being used. Researchers may need to experiment with different concentrations or alternative blocking agents to achieve the best results for their particular application.
Applications
Western Blotting: Used to block non-specific binding sites on membranes before the addition of antibodies.
Immunohistochemistry (IHC): Prevents non-specific binding of antibodies to tissue sections.
Advantages:
More effective than nonfat milk blocking buffers for biotin-avidin systems.
Contains a single purified protein devoid of endogenous biotin.
The addition of Tween-20 helps to further reduce non-specific binding.
The use of BSA helps to minimize background signals, improving the clarity of results.
Composition:
Ingredients
Concentration
BSA
3%
Sodium Chloride
137 mM
Potassium Chloride
2.7 mM
Sodium Phosphate dibasic
10 mM
Potassium Phosphate monobasic
1.8 mM
Tween-20
0.05%
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.4
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: -20 ℃
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
We plan to continue using it.
Jaemin *****
Satisfaction
Quality
Value
Style
Blocking Buffer,
We usually prepare it using powdered BSA, and this is also good.
