PBST with 5% Nonfat Dry Milk
FluxMPS™ PBST with 5% Nonfat Dry Milk
FluxMPS™ PBST with 5% Nonfat Dry Milk is an MPS-grade Western blot blocking and antibody dilution buffer built on a stable, PBS-based pH 7.4 platform. Ultra-filtered through a quadruple-stage 0.1-micron and 0.04-micron membrane process and preserved with ProClin 300, it delivers a sterile, DNase- and RNase-free reagent for immunoblotting, ELISA, and general molecular biology workflows.
- Quadruple-stage ultra-filtration: 0.1-micron membrane twice and 0.04-micron membrane twice
- Sterile, ultrapure formulation confirmed DNase- and RNase-free by QC testing
- Stable PBS-based pH 7.4 buffering for reliable antibody-antigen interactions
- 5% Nonfat Dry Milk blocking agent reduces background banding in Western blots
- 0.05% Tween-20 detergent minimizes non-specific binding
- ProClin 300 preservative (0.05%) extends shelf life
- Ultrapure Type 1 water (18.2 MΩ·cm) formulation base
- Custom concentrations, pH, and additive formulations available on request
- pH7.4
- FormulationPBS + 5% Nonfat Dry Milk + 0.05% Tween-20
- Sodium Chloride137 mM
- Potassium Chloride2.7 mM
- Sodium Phosphate dibasic10 mM
- Potassium Phosphate monobasic1.8 mM
- PreservativeProClin 300, 0.05%
- Filtration0.1 µm x2 + 0.04 µm x2
- Storage4 °C
- Shelf Life1 year
Engineered where standard blocking buffers fail
Conventional 0.22 µm-filtered blocking buffers can carry subvisible particulates, inconsistent pH, and residual bioburden into sensitive immunodetection workflows, contributing to elevated background and lot-to-lot variability. FluxMPS™ buffers are built to a tighter purity and consistency standard from the first fill.[1,2]
Microchannel-safe purity
Quadruple-stage 0.1 µm and 0.04 µm membrane filtration reduces fine particulate carryover compared to a single 0.22 µm pass.
Precise, stable pH
PBS-based formulation is set to pH 7.4, supporting consistent antibody-antigen interactions across replicate blots.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), aligned with USP <85> water-quality expectations.
Low background for imaging & assays
5% Nonfat Dry Milk blocking agent occupies vacant binding sites, improving signal-to-noise ratio in Western blots.
Defined, traceable composition
Every salt, detergent, blocking protein, and preservative is documented at its declared concentration for full traceability.
Customization on demand
Alternate concentrations, additives, or pH available — contact support@diagnocine.com.
Quadruple-stage filtration system
This buffer is filtered 0.1-micron membrane twice and 0.04-micron membrane twice, giving it a four-stage purity architecture built to exclude fine particulates and bioburden before final sterile fill.
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1
0.1 µm Pre-filtration I
First 0.1-micron membrane pass removes large particulates and aggregates, extending the life of downstream filters.
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2
0.04 µm Pre-filtration II
First 0.04-micron membrane pass retains fine particulates and bioburden ahead of the final filtration cycle.
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3
0.1 µm Sterile-filtration I
Second 0.1-micron membrane pass provides redundant clarification before the final polish.
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4
0.04 µm Sterile-filtration II — Final Polish
Second 0.04-micron membrane pass performs the final polish in a sterile environment. The smallest mycoplasma type is about 0.2 µm, so this pore size supports exclusion of mycoplasma contamination.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration passes remove finer particulates than a single 0.22 µm pass, supporting a cleaner, lower-background buffer for immunodetection and general molecular biology work.
© Diagnocine® — DCP-DMB03
Where PBST with 5% Nonfat Dry Milk is used
Formulated as a blocking and antibody dilution buffer for Western blotting, this PBS-Tween/milk solution also supports the broader FluxMPS™ buffer platform's use in immunoassays, sample preparation, and general aqueous reagent needs across life-science research.
Automated Bioreactors & Robotics
For automated dispensing and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of FluxMPS™ buffers is available to further reduce particulate exposure to valves and sensors.
- Total Particulate Exclusion for automated fluid paths
- Valve & Sensor Protection in robotic dispensing systems
- Extended Perfusion Stability for long-duration automated protocols
Inquiry Required: the 0.01 µm (10 nm) ultra-filtered grade is available on request — contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Ultra-filtered aqueous reagent compatible with wash and dilution steps in microfluidic and organ-on-a-chip workflows.
Wash, Dilution & Reconstitution
PBS-based buffer suitable for general wash, dilution, and reconstitution steps across molecular biology workflows.
iPSC-Derived Model Handling
Ultrapure, sterile buffer formulation suitable for supporting wash and dilution steps around iPSC-derived model workflows.
Endothelial & Primary Cell Perfusion
Ultra-filtered, DNase- and RNase-free buffer suitable for general aqueous reagent needs in primary and endothelial cell workflows.
ELISA, Blotting & Blocking
Purpose-built 5% Nonfat Dry Milk and Tween-20 formulation for blocking non-specific binding and diluting primary and secondary antibodies in immunoblotting.
Microscopy & Optical Sensing
Low-particulate, ultrapure buffer suitable for supporting wash and dilution steps in optical and imaging-based assays.
Full specification set
Physical, chemical, sterility, storage, and traceability parameters for DCP-DMB03.
| Parameter | Specification |
|---|---|
| Formulation | PBS-based buffer with 5% Nonfat Dry Milk, 0.05% Tween-20, 0.05% ProClin 300 |
| Appearance | Clear, Colorless Liquid |
| pH | 7.4 |
| Sodium Chloride concentration | 137 mM |
| Potassium Chloride concentration | 2.7 mM |
| Sodium Phosphate dibasic concentration | 10 mM |
| Potassium Phosphate monobasic concentration | 1.8 mM |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Filtered 0.1-micron membrane twice and 0.04-micron membrane twice in a sterile environment |
| DNase Activity | None detected after 18 hr incubation with plasmid DNA at room temperature |
| RNase Activity | None detected after 18 hr incubation with ribosomal RNA at room temperature |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved manufacturing facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4 °C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified, CE-approved facility (Suppliers of DiagnoCine Precision) |
| Traceability | Final packaging, QA, and testing performed at the DiagnoCine R&D and Quality Testing Center |
| Production Method | Custom formulation and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | For Research Use Only (RUO) |
Full composition
Every declared ingredient and concentration in DCP-DMB03, reproduced in full below.
| Component | CAS Number | Concentration |
|---|---|---|
| Nonfat Dry Milk | 5% | |
| ProClin 300 | 0.05% | |
| Potassium Chloride (KCl) | 7447-40-7 | 2.7 mM |
| Sodium Chloride (NaCl) | 7647-14-5 | 137 mM |
| Sodium Phosphate dibasic (Na2HPO4) | 7558-79-4 | 10 mM |
| Potassium Phosphate monobasic (KH2PO4) | 7778-77-0 | 1.8 mM |
| Tween-20 (Polysorbate 20) | 9005-64-5 | 0.05% |
Manufacturing & compliance
DCP-DMB03 is manufactured, filtered, and packaged under a controlled, certified quality system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base.
Sterile, Quadruple-Stage Filtration
Filtered 0.1-micron membrane twice and 0.04-micron membrane twice in a sterile environment.
Micro-Batch Precision
Final packaging, quality assurance, and testing are performed at the DiagnoCine R&D and Quality Testing Center.
DNase Activity
None detected after incubation of plasmid DNA with this product for 18 hr at room temperature.
RNase Activity
No RNase activity detected after incubation of ribosomal RNA with this product for 18 hr at room temperature.
Appearance & pH
Clear, colorless liquid at pH 7.4.
Documentation / CoA
Custom customization requests and assembly are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
How DCP-DMB03 compares
FluxMPS™ quadruple-stage filtered buffer versus conventional 0.22 µm-filtered alternatives.
| Parameter | DCP-DMB03 (FluxMPS™) | Conventional Buffer | Standard Alternative |
|---|---|---|---|
| Stable, PBS-based pH control | check_circle pH 7.4 | Not specified | Not specified |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| DNase/RNase-free confirmed | check_circle | cancel | cancel |
| Ultrapure Type 1 water base | check_circle | cancel | cancel |
| ISO 13485:2016 manufacturing | check_circle | cancel | cancel |
| Microfluidic / microchannel compatibility | check_circle | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-DMB03, PBST with 5% Nonfat Dry Milk.
Supporting literature
Curated peer-reviewed and standards references relevant to Western blot blocking chemistry, buffer purity, and microfluidic reagent use.
- Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012. doi:10.4103/1947-2714.100998
- Kurien BT, Scofield RH. Western blotting. Methods. 2006. doi:10.1016/j.ymeth.2005.11.007
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Eaton SL, et al. Total protein analysis as a reliable loading control for quantitative western blotting. PLoS One. 2013. doi:10.1371/journal.pone.0072457
- United States Pharmacopeia. General Chapter <71> Sterility Tests. USP-NF.
- United States Pharmacopeia. General Chapter <85> Bacterial Endotoxins Test. USP-NF.
- United States Pharmacopeia. General Chapter <788> Particulate Matter in Injections. USP-NF.
- International Organization for Standardization. ISO 13485:2016 Medical devices — Quality management systems.
- Halfter K, et al. Choice of blocking reagent influences the sensitivity of protein detection in western blotting. Anal Biochem. 2010.
