PBST with Bovine Serum Albumin (3%) Blocking Buffer
FluxMPS™ PBST with Bovine Serum Albumin (3%) Blocking Buffer
An MPS-grade, sterile PBST blocking buffer formulated with 3% Bovine Serum Albumin (BSA) and 0.05% Tween-20 for Western blotting, ELISA, and immunohistochemistry. Manufactured with quadruple-stage filtration (0.1 µm membrane twice and 0.04 µm membrane twice) in Ultrapure Type 1 water for ultra-low particulate, low-background immunoassay and microfluidic workflows.
- Quadruple-stage filtration: 0.1 µm membrane twice and 0.04 µm membrane twice for ultra-clean, microchannel-safe purity
- 3% BSA blocking protein — a single purified protein devoid of endogenous biotin, ideal for biotin-avidin detection systems
- Formulated in phosphate buffered saline with 0.05% Tween-20 to reduce surface tension and non-specific background
- Sterile; DNase- and RNase-tested for confident use in molecular biology protocols
- Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm)
- Precise, controlled pH 7.4 formulation
- Manufactured under ISO 13485-certified, CE-approved facilities
- Customizable concentration, pH, and additive content available on request
- pH7.4
- Blocking ProteinBSA, 3%
- DetergentTween-20, 0.05%
- Sodium Chloride137 mM
- Potassium Chloride2.7 mM
- Filtration0.1 µm x2 + 0.04 µm x2
- SterilitySterile, filtered
- AppearanceClear, colorless liquid
- Storage-20°C
- Shelf Life1 year
Engineered where standard blocking buffers fail
Conventional 0.22 µm-filtered blocking buffers can carry subvisible particulates, endogenous biotin, and inconsistent pH into sensitive immunoassays and microfluidic channels, elevating background signal and clogging fine-bore systems. FluxMPS™ PBST with 3% BSA is built to remove those failure points at the source.
Microchannel-safe purity
Sequential 0.1 µm and 0.04 µm filtration passes yield an ultra-low particulate buffer suited to fine-bore microfluidic and organ-on-a-chip channels.
Precise, stable pH
Formulated to a defined pH of 7.4 in phosphate buffered saline, supporting reproducible blocking performance across assays.
Ultrapure-grade water
Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm), aligned with USP <85> water quality expectations.
Low background for imaging & assays
A single purified BSA protein free of endogenous biotin reduces non-specific background in Western blot, ELISA, and IHC.
Defined, traceable composition
Every salt, protein, and detergent component is manufactured and released against a fixed formulation of PBS, 3% BSA, and 0.05% Tween-20.
Customization on demand
Concentration, pH, and additional chemicals, compounds, proteins, or supplements can be added on request.
Quadruple-stage filtration system
FluxMPS™ PBST with 3% BSA Blocking Buffer is sterile-filtered through a quadruple-stage membrane sequence — 0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice — in a sterile environment, supporting an ultra-clean, low-particulate buffer for demanding immunoassay and microfluidic workflows.
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1
0.1 µm Pre-filtration I
Removes large particulates and aggregates, protecting downstream membranes and extending filter life.
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2
0.04 µm Pre-filtration II
Retains fine particulates and bioburden approaching the size range of the smallest mycoplasma organisms, which can be about 0.2 microns.
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3
0.1 µm Sterile-filtration I
A second 0.1 µm pass provides redundant sterile filtration ahead of final polishing.
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4
0.04 µm Sterile-filtration II — Final Polish
A second 0.04 µm pass delivers the final sterile polish, filled in a sterile environment to help prevent mycoplasma contamination.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration passes remove finer particulates than a single 0.22 µm filtration step typical of conventional blocking buffers, supporting cleaner background in downstream immunoassays and microfluidic channels.
© Diagnocine® — DCP-BSAPBST3X
Built for immunoassay, microfluidic & MPS workflows
FluxMPS™ PBST with 3% BSA Blocking Buffer blocks non-specific binding sites prior to antibody incubation in Western blotting, enhances signal-to-noise in ELISA, and prevents non-specific antibody binding to tissue sections in immunohistochemistry (IHC).
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant is available on request for automated bioreactor and robotic liquid-handling systems where fine-bore valves and sensors demand additional particulate exclusion.
- Total Particulate Exclusion: further reduction of subvisible particulates beyond the standard quadruple-stage architecture
- Valve & Sensor Protection: helps protect fine-bore automated fluidic valves and optical sensors
- Extended Perfusion Stability: supports longer uninterrupted runs in automated perfusion systems
Inquiry Required: contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade.
Micro Physiological System (MPS) & Chip
Low-particulate, low-background blocking buffer suited to microfluidic immunostaining workflows.
Wash, Dilution & Reconstitution
PBST base with 0.05% Tween-20 supports wash and dilution steps ahead of protein detection assays.
iPSC-Derived Model Handling
DNase- and RNase-tested formulation suited to blocking steps in iPSC-derived immunostaining protocols.
Endothelial & Primary Cell Perfusion
Ultra-clean, sterile-filtered buffer for blocking steps in endothelial and primary cell immunostaining.
ELISA, Blotting & Blocking
A biotin-free, purified BSA blocking protein improves specificity and sensitivity in Western blotting, ELISA, and IHC.
Microscopy & Optical Sensing
Low-background blocking chemistry supports cleaner signal in fluorescence-based imaging and biosensing.
Full technical specifications
Every parameter below reflects the manufactured, tested formulation of DCP-BSAPBST3X.
| Parameter | Specification |
|---|---|
| Formulation / Composition | PBS with 3% BSA and 0.05% Tween-20 |
| Appearance | Clear, colorless liquid |
| pH (USP <791>) | 7.4 |
| Sodium Chloride | 137 mM |
| Potassium Chloride | 2.7 mM |
| Sodium Phosphate dibasic (Na2HPO4) | 10 mM |
| Potassium Phosphate monobasic (KH2PO4) | 1.8 mM |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment |
| DNase Activity | None detected after incubation of plasmid DNA and this product for 18 hr at room temperature |
| RNase Activity | None detected after incubation of ribosomal RNA and this product for 18 hr at room temperature |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facilities |
| Fill Environment | Sterile environment |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Blocking Protein Grade | Single purified BSA protein, devoid of endogenous biotin |
| Manufacturing QMS | ISO 13485:2016 |
| Regulatory Alignment | CE-approved facilities |
| Production & Traceability | Final packaging, quality assurance, and testing performed at the Diagnocine R&D and Quality Testing Center; customization assembled at Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
PBST with 3% BSA Blocking Buffer is manufactured and lot-released against the fixed formulation below.
| Component | CAS Number | Concentration |
|---|---|---|
| BSA (Bovine Serum Albumin) | 9048-46-8 | 3% |
| Sodium Chloride | 7647-14-5 | 137 mM |
| Potassium Chloride | 7447-40-7 | 2.7 mM |
| Sodium Phosphate dibasic (Na2HPO4) | 7558-79-4 | 10 mM |
| Potassium Phosphate monobasic (KH2PO4) | 7778-77-0 | 1.8 mM |
| Tween-20 | 9005-64-5 | 0.05% |
Manufacturing & compliance
DCP-BSAPBST3X is manufactured, filled, and tested under a documented quality system spanning raw material sourcing through final release.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm).
Sterile Fill & Finish
Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment.
Micro-Batch Precision
Final packaging, quality assurance, and testing performed at the Diagnocine R&D and Quality Testing Center.
DNase/RNase Testing
No DNase or RNase activity detected after 18 hr incubation with plasmid DNA and ribosomal RNA, respectively, at room temperature.
Sterility (USP <71>)
Filtered and filled in a sterile environment to help prevent mycoplasma contamination.
pH Control (USP <791>)
Manufactured to a defined pH of 7.4.
Documentation / CoA
Certificate of Analysis available for this lot.
How DCP-BSAPBST3X compares
A side-by-side view of the FluxMPS™ quadruple-stage BSA-PBST blocking buffer against conventional 0.22 µm-filtered blocking buffer formats.
| Parameter | DCP-BSAPBST3X (FluxMPS™) | Conventional 0.22 µm-Filtered Buffer | Standard Alternative (Nonfat Milk Blocking) |
|---|---|---|---|
| Blocking Protein | 3% single purified BSA, biotin-free | Undefined protein blend | Nonfat milk (contains endogenous biotin) |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | Not filtered |
| Number of Filtration Stages | 4 | 1 | N/A |
| DNase/RNase Tested | check_circle | cancel | cancel |
| Biotin-Avidin Compatible | check_circle | cancel | cancel |
| Manufacturing QMS (ISO 13485) | check_circle | cancel | cancel |
| Microfluidic / OoC Channel Compatibility | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Answers to common questions about DCP-BSAPBST3X.
Supporting literature
Curated references relevant to BSA blocking chemistry, immunoassay methods, and microfluidic / organ-on-a-chip applications.
- Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976. doi:10.1016/0003-2697(76)90527-3
- Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA. 1979. doi:10.1073/pnas.76.9.4350
- Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA): quantitative assay of immunoglobulin G. Immunochemistry. 1971. doi:10.1016/0019-2791(71)90454-X
- Vogt RF, Phillips DL, Henderson LO, Whitfield W, Spierto FW. Quantitative differences among various proteins as blocking agents for ELISA microtiter plates. J Immunol Methods. 1987. doi:10.1016/0022-1759(87)90253-9
- Bordeaux J, Welsh A, Agarwal S, et al. Antibody validation. Biotechniques. 2010. doi:10.2144/000113610
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010. doi:10.1126/science.1188302
- Wu MH, Huang SB, Lee GB. Microfluidic cell culture systems for drug research. Lab Chip. 2010. doi:10.1039/B909900J
We plan to continue using it.
Jaemin *****
Blocking Buffer,
We usually prepare it using powdered BSA, and this is also good.
We plan to continue using it.
