PBST with Bovine Serum Albumin (3%) Blocking Buffer
PBST with Bovine Serum Albumin (3%) Blocking Buffer
A research-grade, nonsterile PBST blocking buffer formulated with 3% Bovine Serum Albumin (BSA) and 0.05% Tween-20 in Ultrapure Type 1 water for Western blotting, ELISA, and immunohistochemistry. 0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
- 0.1 µm membrane filtration removes large particulates and aggregates
- 3% BSA blocking protein — a single purified protein devoid of endogenous biotin, ideal for biotin-avidin detection systems
- Formulated in phosphate buffered saline with 0.05% Tween-20 to reduce surface tension and non-specific background
- Nonsterile; DNase- and RNase-tested for confident use in molecular biology protocols
- Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm)
- Precise, controlled pH 7.4 formulation
- Manufactured under ISO 13485-certified facilities
- Customizable concentration, pH, and additive content available on request
- pH7.4
- Blocking ProteinBSA, 3%
- DetergentTween-20, 0.05%
- Sodium Chloride137 mM
- Potassium Chloride2.7 mM
- Filtration0.1 µm membrane filtration (single step)
- SterilityNonsterile
- AppearanceClear, colorless liquid
- Storage-20°C
- Shelf Life1 year
Engineered where standard blocking buffers fail
Particulates, endogenous biotin, and inconsistent pH can raise background signal in sensitive immunoassays. PBST with 3% BSA is built to address those failure points at the source.
0.1 µm filtered
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
Precise, stable pH
Formulated to a defined pH of 7.4 in phosphate buffered saline, supporting reproducible blocking performance across assays.
Ultrapure-grade water
Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm), aligned with USP <85> water quality expectations.
Low background for imaging & assays
A single purified BSA protein free of endogenous biotin reduces non-specific background in Western blot, ELISA, and IHC.
Defined, traceable composition
Every salt, protein, and detergent component is manufactured and released against a fixed formulation of PBS, 3% BSA, and 0.05% Tween-20.
Customization on demand
Concentration, pH, and additional chemicals, compounds, proteins, or supplements can be added on request.
0.1 µm membrane filtration
PBST with 3% BSA Blocking Buffer undergoes 0.1 µm membrane filtration.
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0.1 µm Membrane Filtration
Removes large particulates and aggregates, helping protect downstream applications.
Filtration performance
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
© Diagnocine® — DCP-BSAPBST3X
Built for immunoassay workflows
PBST with 3% BSA Blocking Buffer blocks non-specific binding sites prior to antibody incubation in Western blotting, enhances signal-to-noise in ELISA, and prevents non-specific antibody binding to tissue sections in immunohistochemistry (IHC).
Dot Blot & Protein Arrays
Blocks unoccupied binding sites on membranes before antibody probing in dot blot and protein array formats.
Wash, Dilution & Reconstitution
PBST base with 0.05% Tween-20 supports wash and dilution steps ahead of protein detection assays.
iPSC-Derived Model Handling
DNase- and RNase-tested formulation suited to blocking steps in iPSC-derived immunostaining protocols.
Endothelial & Primary Cells
Blocking buffer for endothelial and primary cell immunostaining.
ELISA, Blotting & Blocking
A biotin-free, purified BSA blocking protein improves specificity and sensitivity in Western blotting, ELISA, and IHC.
Microscopy & Optical Sensing
Low-background blocking chemistry supports cleaner signal in fluorescence-based imaging and biosensing.
Full technical specifications
Every parameter below reflects the manufactured, tested formulation of DCP-BSAPBST3X.
| Parameter | Specification |
|---|---|
| Formulation / Composition | PBS with 3% BSA and 0.05% Tween-20 |
| Appearance | Clear, colorless liquid |
| pH (USP <791>) | 7.4 |
| Sodium Chloride | 137 mM |
| Potassium Chloride | 2.7 mM |
| Sodium Phosphate dibasic (Na2HPO4) | 10 mM |
| Potassium Phosphate monobasic (KH2PO4) | 1.8 mM |
| Parameter | Specification |
|---|---|
| Sterility | Nonsterile |
| Filtration | 0.1 µm membrane filtration (single step) |
| DNase Activity | None detected after incubation of plasmid DNA and this product for 18 hr at room temperature |
| RNase Activity | None detected after incubation of ribosomal RNA and this product for 18 hr at room temperature |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified facilities |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Blocking Protein Grade | Single purified BSA protein, devoid of endogenous biotin |
| Manufacturing QMS | ISO 13485:2016 |
| Production & Traceability | Final packaging, quality assurance, and testing performed at the Diagnocine R&D and Quality Testing Center; customization assembled at Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
PBST with 3% BSA Blocking Buffer is manufactured and lot-released against the fixed formulation below.
| Component | CAS Number | Concentration |
|---|---|---|
| BSA (Bovine Serum Albumin) | 9048-46-8 | 3% |
| Sodium Chloride | 7647-14-5 | 137 mM |
| Potassium Chloride | 7447-40-7 | 2.7 mM |
| Sodium Phosphate dibasic (Na2HPO4) | 7558-79-4 | 10 mM |
| Potassium Phosphate monobasic (KH2PO4) | 7778-77-0 | 1.8 mM |
| Tween-20 | 9005-64-5 | 0.05% |
Manufacturing & compliance
DCP-BSAPBST3X is manufactured, filled, and tested under a documented quality system spanning raw material sourcing through final release.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified facilities.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm).
Filtration
0.1 µm membrane filtration (single step).
Micro-Batch Precision
Final packaging, quality assurance, and testing performed at the Diagnocine R&D and Quality Testing Center.
DNase/RNase Testing
No DNase or RNase activity detected after 18 hr incubation with plasmid DNA and ribosomal RNA, respectively, at room temperature.
Sterility
Nonsterile.
pH Control (USP <791>)
Manufactured to a defined pH of 7.4.
Documentation / CoA
Certificate of Analysis available for this lot.
How DCP-BSAPBST3X compares
A side-by-side view of DCP-BSAPBST3X against conventional blocking buffer formats.
| Parameter | DCP-BSAPBST3X | Conventional Blocking Buffer | Standard Alternative (Nonfat Milk Blocking) |
|---|---|---|---|
| Blocking Protein | 3% single purified BSA, biotin-free | Undefined protein blend | Nonfat milk (contains endogenous biotin) |
| DNase/RNase Tested | check_circle | cancel | cancel |
| Biotin-Avidin Compatible | check_circle | cancel | cancel |
| Manufacturing QMS (ISO 13485) | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Answers to common questions about DCP-BSAPBST3X.
Supporting literature
Curated references relevant to BSA blocking chemistry and immunoassay methods.
- Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976. doi:10.1016/0003-2697(76)90527-3
- Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA. 1979. doi:10.1073/pnas.76.9.4350
- Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA): quantitative assay of immunoglobulin G. Immunochemistry. 1971. doi:10.1016/0019-2791(71)90454-X
- Vogt RF, Phillips DL, Henderson LO, Whitfield W, Spierto FW. Quantitative differences among various proteins as blocking agents for ELISA microtiter plates. J Immunol Methods. 1987. doi:10.1016/0022-1759(87)90253-9
- Bordeaux J, Welsh A, Agarwal S, et al. Antibody validation. Biotechniques. 2010. doi:10.2144/000113610
We plan to continue using it.
Jaemin *****
Blocking Buffer,
We usually prepare it using powdered BSA, and this is also good.
We plan to continue using it.
