Non-Sterile; Ultrapure, Filtered 0.1-micron membrane Once and 0.04-micron membrane Once.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
Phosphate-buffered saline (PBS) with Triton X-100 is a commonly used buffer solution in biological research, particularly for applications that require cell lysis and protein solubilization. Triton X-100 is a nonionic detergent, which makes it less harsh than ionic detergents. This mildness helps in solubilizing membrane proteins while preserving protein-protein interactions and maintaining proteins in their native state. Due to its non-denaturing properties, Triton X-100 helps retain the functionality of proteins during the washing or lysis process. This is crucial for studies involving protein interactions or when the native structure of proteins needs to be preserved. The PBS component provides a stable pH environment, which is essential for maintaining protein stability during experiments. PBS typically maintains a physiological pH of around 7.4, which is ideal for many biological assays. This buffer is suitable for a variety of applications, including cell lysis, immunoprecipitation, and protein extraction. It is particularly useful in preparing cell extracts for techniques like Western blotting and ELISA.
Applications
Cell Permeabilization: Triton X-100 is a nonionic detergent that effectively permeabilizes cell membranes.
Protein Extraction: The buffer is commonly used for lysing cells to extract proteins. The combination of PBS and Triton X-100 helps solubilize membrane proteins while preserving their functional state, making it suitable for downstream applications like Western blotting and ELISA.
Washing Steps in Assays: PBS with Triton X-100 is often used as a wash buffer in various assays, including ELISA and Western blotting. The detergent helps reduce background noise by washing away unbound antibodies or other reagents.
Immunostaining: In immunohistochemistry protocols, PBS with Triton X-100 is used to permeabilize fixed tissues, facilitating better antibody access to target antigens.
Cell Culture Applications: The buffer can be used to wash cells before and after treatments, helping to maintain physiological conditions while preparing samples for further analysis.
DNA Extraction: In some DNA extraction protocols, PBS with Triton X-100 is used to lyse cells and release nucleic acids for subsequent purification steps.
Advantages:
Mimics Physiological Conditions: PBS closely mimics the physiological pH, osmolarity, and ion concentrations of the human body, providing an optimal environment for cell survival and function during experimental procedures. This reduces the risk of cellular stress or damage.
The combination of PBS and Triton X-100 is commonly used for lysing cells to extract proteins while preserving their functional state.
It helps reduce background noise by washing away unbound antibodies or other reagents.
Composition:
Ingredients
Concentration
Sodium Chloride
137 mM
Potassium Chloride
2.7 mM
Sodium Phosphate dibasic
10 mM
Potassium Phosphate monobasic
1.8 mM
Triton X-100
1%
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.4
Sterility: Filtered 0.1-micron membrane Once and 0.04-micron membrane Once.
Storage and Shelf Life:
Storage: 4 ℃
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
