PBS NP-40 Buffer [1X]
Cat No.: DCP-PBSNP1X
Size: 500 mL & 1 L
Non-Sterile; Ultrapure, Filtered 0.1-micron membrane Once and 0.04-micron membrane Once.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
PBS with NP-40 is a versatile lysis buffer that balances effective protein extraction with preservation of protein structure, making it a good choice for many western blot applications. PBS with NP-40 buffer is primarily used for cell lysis and protein extraction in western blotting and other protein analysis techniques. Phosphate buffered saline helps to maintain physiological pH and osmolarity. It also provides a stable environment for proteins. NP-40 is a non-ionic detergent which disrupts cell membranes while preserving protein-protein interactions. NP-40 helps solubilize membrane-bound and cytoplasmic proteins. It forms micelles around hydrophobic regions of proteins, keeping them in solution. This allows cellular contents to be released into the buffer. As a mild detergent, NP-40 generally doesn't denature proteins. This helps maintain protein-protein interactions and enzyme activity.
Applications
- Cell Lysis: Effectively disrupts cell membranes to release cellular contents. Suitable for lysing cultured cells and tissue samples.
- Protein Extraction: Efficiently extracts cytoplasmic and membrane-bound proteins. Preserves protein-protein interactions better than harsher detergents.
- Sample Preparation: Used to prepare whole cell lysates for western blotting. Can be used for immunoprecipitation experiments.
- Washing steps in immunoassays.
Advantages:
- Mild non-ionic detergent (NP-40) helps solubilize proteins without denaturing them
- Preserves protein-protein interactions better than harsher ionic detergents
- Effective for extracting most cytoplasmic and membrane proteins
- Compatible with many downstream applications, including immunoprecipitation
Composition:
| Ingredients |
Concentration |
| Sodium Chloride |
137 mM |
| Potassium Chloride |
2.7 mM |
| Sodium Phosphate dibasic |
10 mM |
| Potassium Phosphate monobasic |
1.8 mM |
| NP-40 |
1% |
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.4
Sterility: Filtered 0.1-micron membrane Once and 0.04-micron membrane Once.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: 4 ℃
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
