Orange Loading Buffer [6X]
Cat No.: DCP-OLB6X
Size: 5 x 1 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Orange Loading Buffer facilitates the loading process by increasing the density of the sample and providing visual tracking. It is recommended for DNA fragments smaller than 500 bp. The Orange G dye runs ahead of most DNA samples, ensuring it doesn't obscure visualization of DNA bands. When added to the DNA sample, it increases the overall density of the mixture relative to the buffer. The increased density helps the DNA sample sink to the gel wells' bottom during loading rather than diffusing into the surrounding running buffer. Tris-HCl provides a stable pH environment for the DNA/RNA samples and helps maintain the integrity of nucleic acids during electrophoresis. EDTA acts as a chelating agent and inhibits metal-dependent nucleases, protecting DNA/RNA from degradation. Orange G serves as a tracking dye and allows visual monitoring of the electrophoresis progress. Glycerol increases the density of the sample and ensures that the DNA/RNA sample sinks to the bottom of the well when loaded.
* Please note that this buffer contains Orange G dye.
Advantages:
- Increased sample density: Glycerol in the buffer ensures the DNA sample sinks to the bottom of the well, preventing sample loss.
- DNA protection: EDTA in the buffer binds divalent metal ions, inhibiting metal-dependent nucleases and protecting DNA from degradation.
- Compatibility: Works with various DNA staining methods and can be used with both agarose and polyacrylamide gels.
- No DNA masking: The dyes used do not interfere with DNA visualization under UV light.
Applications:
- Agarose gel electrophoresis for DNA fragment analysis.
- PCR product visualization.
- Restriction enzyme digest analysis.
- DNA quantification and quality assessment.
Composition:
| Ingredients |
Concentration |
| Glycerol |
50% |
| Orange G dye |
0.25% |
| Tris-HCl |
10 mM |
| EDTA |
60mM |
Quality Control:
-
Appearance: Orange color.
-
pH: 7.6
-
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
-
DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
-
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
-
Storage: -20 ℃
-
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
