Orange Loading Buffer [6X]
FluxMPS™ Orange Loading Buffer [6X]
FluxMPS™ Orange Loading Buffer [6X] delivers a precise pH 7.6 Tris-HCl/EDTA formulation with defined 10 mM / 60mM molarity, ultra-filtered through a quadruple-stage 0.1 µm (x2) and 0.04 µm (x2) membrane process for microchannel-safe, low-background performance in DNA and RNA electrophoresis workflows. Orange G dye and glycerol provide reliable sample tracking and loading density without obscuring band visualization.
- Quadruple-stage 0.1 µm (x2) and 0.04 µm (x2) membrane filtration — sterile, ultrapure formulation
- Increased sample density via glycerol for reliable well loading
- EDTA chelation protects DNA/RNA from metal-dependent nuclease degradation
- Orange G tracking dye runs ahead of most DNA samples without obscuring band visualization
- Compatible with both agarose and polyacrylamide gels
- DNase- and RNase-activity tested (none detected after 18-hour room-temperature incubation)
- Customization available — alternate pH, tracking dyes, and concentrations on request
- pH7.6
- Tris-HCl10 mM
- EDTA60mM
- Glycerol50%
- Orange G Dye0.25%
- AppearanceOrange color
- Sterility0.1 µm x2 + 0.04 µm x2
- DNase/RNase ActivityNone detected
- Storage-20°C
- Shelf Life1 year
Engineered where standard loading buffers fail
Conventional 0.22 µm-filtered loading buffers can carry subvisible particulates, inconsistent tracking dye behavior, and unverified nuclease activity into sensitive DNA/RNA electrophoresis workflows. FluxMPS™ Orange Loading Buffer [6X] is engineered to remove those failure points at the source.
Microchannel-safe purity
Quadruple-stage 0.1 µm (x2) and 0.04 µm (x2) membrane filtration removes particulates that can interfere with fine capillary electrophoresis and lab-on-chip DNA analysis systems[1].
Precise, stable pH
Tris-HCl buffering at pH 7.6 provides a stable environment that helps maintain the integrity of DNA and RNA samples during electrophoresis.
Ultrapure-grade water
Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm) consistent with USP <85> manufacturing practices.
Low background for imaging & assays
Orange G dye runs ahead of most DNA samples under 500 bp, ensuring it does not obscure visualization of DNA bands under UV or blue-light imaging.
Defined, traceable composition
Glycerol, Orange G dye, Tris-HCl, and EDTA concentrations are controlled and documented on a per-lot basis.
Customization on demand
Alternate concentrations, additional tracking dyes, different pH, and other modifications are available — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of Orange Loading Buffer [6X] is sterile-filtered with two passes through a 0.1 µm membrane and two passes through a 0.04 µm membrane, in a sterile environment, delivering an ultrapure loading buffer for demanding molecular biology workflows.
- 1
0.1 µm Pre-filtration I
Removes large particulates and aggregates from the Tris-HCl/EDTA/glycerol/Orange G formulation, extending downstream filter life and protecting fine gel-loading applications from debris.
- 2
0.04 µm Pre-filtration II
A finer 0.04 µm membrane pass retains fine particulates and bioburden ahead of the final sterile-filtration stages.
- 3
0.1 µm Sterile-filtration I
A second 0.1 µm pass provides redundant particulate and bioburden reduction before final polishing.
- 4
0.04 µm Sterile-filtration II — Final Polish
The second 0.04 µm pass delivers the final polish in a sterile, ISO Class 5 (Class 100) fill environment. Sequential dual-membrane filtration reduces bioburden below the size of the smallest known mycoplasma organisms, reported at approximately 0.2 micron, consistent with DiagnoCine Precision's dual-membrane sterility standard[3].
Performance vs. conventional buffer
Sequential 0.1 µm (x2) and 0.04 µm (x2) membrane filtration removes finer particulates than the single-pass 0.22 µm filtration typical of standard loading buffers, supporting cleaner downstream electrophoresis, imaging, and DNA/RNA visualization.
© Diagnocine® — DCP-OLB6X
Built for demanding molecular biology workflows
Orange Loading Buffer [6X] facilitates the loading process by increasing sample density and providing visual tracking for DNA fragments smaller than 500 bp.
Automated Bioreactors & Robotics
For automated liquid-handling and capillary electrophoresis platforms, an optional 0.01 µm (10 nm) ultra-filtered variant is available on request to further protect fine valves, sensors, and microfluidic pathways in robotics-driven genomic workstations.
- Total Particulate Exclusion: further reduction of sub-micron particulate for automated pipetting and dispensing systems
- Valve & Sensor Protection: reduced particulate load helps protect fine microfluidic valves and optical sensors
- Extended System Uptime: cleaner reagent input supports fewer clogs and less maintenance downtime in automated platforms
Inquiry Required: the 0.01 µm (10 nm) ultra-filtered grade is produced on request — contact support@diagnocine.com.
Agarose & Polyacrylamide Gel Loading
Increases sample density and provides visual tracking for reliable well loading on both agarose and polyacrylamide gels.
PCR Product Visualization
Orange G tracking dye monitors electrophoresis progress without obscuring visualization of amplicon bands.
Restriction Enzyme Digest Analysis
Supports confirmation of restriction digest fragment patterns during vector construction and plasmid QC.
DNA/RNA Quantification & Quality Assessment
Compatible with standard DNA staining methods for downstream quantification and quality assessment.
Automated Electrophoresis & Capillary Systems
Ultra-filtered purity supports use in automated capillary electrophoresis and lab-on-chip nucleic acid analysis platforms.
UV/Blue-Light Gel Documentation
The dyes used do not interfere with DNA visualization under UV light, supporting clean gel documentation imaging.
Full technical profile
Physical, chemical, purity, storage, and regulatory parameters for Orange Loading Buffer [6X], DCP-OLB6X.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Glycerol, Orange G dye, Tris-HCl, EDTA |
| Appearance | Orange color |
| pH (USP <791>) | 7.6 |
| Molarity / Concentration | Tris-HCl 10 mM; EDTA 60mM |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment |
| DNase Activity | None detected after incubation with plasmid DNA for 18 hours at room temperature |
| RNase Activity | None detected after incubation with ribosomal RNA for 18 hours at room temperature |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Fill Environment | ISO Class 5 (Class 100) aseptic fill |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facilities |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO 13485:2016 | ISO 13485-certified suppliers |
| Regulatory Alignment | CE-approved facilities |
| Traceability | Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center; customization and assembly performed at DiagnoCine Precision, Totowa, New Jersey, USA |
| Production Method | Sequential 0.1 µm (x2) and 0.04 µm (x2) membrane filtration |
| Intended Use | For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans. |
Full composition
Orange Loading Buffer [6X] is a single-system Tris-HCl/EDTA buffer combined with glycerol and Orange G tracking dye. All components are manufactured and released on a per-lot basis.
| Component | CAS Number | Concentration |
|---|---|---|
| Glycerol | 56-81-5 | 50% |
| Orange G dye | 1936-15-8 | 0.25% |
| Tris-HCl | 1185-53-1 | 10 mM |
| EDTA | 60-00-4 | 60mM |
Manufacturing & compliance
Orange Loading Buffer [6X] is manufactured under ISO 13485-certified and CE-approved facilities, with final packaging, quality assurance, and testing completed at the DiagnoCine R&D and Quality Testing Center.
Sterility USP <71>
Verified via sequential 0.1 µm and 0.04 µm dual-membrane filtration in a sterile environment.
DNase/RNase Activity Testing
None detected after 18-hour incubation with plasmid DNA and ribosomal RNA at room temperature.
Documentation / CoA
Certificate of Analysis available detailing appearance, pH, sterility/filtration, and DNase/RNase results.
CE-Approved Facilities
Manufactured under ISO 13485-certified and CE-approved supplier facilities.
How DCP-OLB6X compares
Orange Loading Buffer [6X] against conventional single-pass filtered loading buffers.
| Parameter | DCP-OLB6X (FluxMPS™) | Conventional Loading Buffer | Standard Alternative (0.22 µm filtered) |
|---|---|---|---|
| Filtration architecture | Quadruple-stage (0.1 µm x2 + 0.04 µm x2) | Single 0.22 µm pass | Single 0.22 µm pass |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Sterile-filtered manufacturing | check_circle | cancel | cancel |
| DNase/RNase tested | check_circle None detected | cancel | cancel |
| Manufacturing QMS | ISO 13485:2016 | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Standard purified water | Standard purified water |
Frequently asked questions
Common questions about Orange Loading Buffer [6X], DCP-OLB6X.
Supporting literature
Curated literature relevant to buffer chemistry, tracking dyes, and sterile filtration referenced in this description.
- Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual, 3rd ed. Tris-EDTA buffer systems for nucleic acid electrophoresis. doi:10.1101/pdb.top75
- Voet D, Voet JG. Biochemistry. EDTA as a chelating agent and inhibitor of metal-dependent nucleases. doi:10.1002/9780470117951
- Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev. doi:10.1128/MMBR.62.4.1094-1156.1998
- Sigma-Aldrich Technical Bulletin. Orange G as a gel loading tracking dye. doi:10.1016/j.ab.2010.01.001
- Lee PY, Costumbrado J, Hsu CY, Kim YH. Agarose gel electrophoresis for the separation of DNA fragments. J Vis Exp. doi:10.3791/3923
- ISO 13485:2016 Medical devices - Quality management systems - Requirements for regulatory purposes. doi:10.3403/30295400
- United States Pharmacopeia. USP <71> Sterility Tests. doi:10.31003/USPNF_M99105_02_01
- Green MR, Sambrook J. Analysis of DNA by agarose gel electrophoresis and gel documentation imaging. Cold Spring Harb Protoc. doi:10.1101/pdb.top093435
