OmniFix Tissue Clearing Kit
FluxMPS™ OmniFix Tissue Clearing Kit
The FluxMPS™ OmniFix Tissue Clearing Kit (Cat. No. DCP-OTCK) delivers optical clarity across a broad range of pre-fixed tissue samples through a precision 0.1 µm single-stage filtration process — providing ultra-low particulate, ready-to-use reagents engineered for deep imaging, enhanced antibody penetration, and reproducible histological results in diverse research workflows.
- 0.1 µm single-stage filtration: DiagnoCine Precision™ filtered-sterilized through a 0.1-micron membrane once, surpassing standard 0.22 µm filtration for ultra-low particulate purity.
- Broad fixation compatibility: Validated for EMA, PFA, formalin, ethanol/methanol, and glutaraldehyde-fixed tissues.
- Ready-to-use dual-solution system: Solution A (EZ View Solution) + Solution B (TF Lipid Removal Solution) pre-formulated for minimal preparation time and user error.
- High transparency: Rapidly transforms opaque samples into optically clear tissues while preserving structural integrity.
- Antigen preservation: Maintains protein epitopes and fluorescent signals for reliable immunostaining and advanced microscopy.
- Versatile imaging support: Compatible with confocal, light-sheet, and wide-field fluorescence microscopy as well as histological analysis.
- ISO 13485 manufacturing: CE-approved facilities, assembled at DiagnoCine Precision, Totowa, New Jersey, USA.
- Customizable: pH, concentration, additives, and modifications available on request — contact support@diagnocine.com.
- Solution A (EZ View Solution)100 mL | pH 7.4 ± 0.04
- Solution B (TF Lipid Removal Solution)100 mL | pH 6–7
- AppearanceClear-colorless solution
- Filtration0.1 µm membrane, once
- Sterility MethodNon-sterile environment fill
- StorageRoom Temperature
- Shelf Life — Solution A6 months
- Shelf Life — Solution B1 month
- Post-clearing (short-term)RT, up to 1 week in EZ View
- Post-clearing (long-term)RT, up to 70 days in EZ View
Engineered where conventional clearing methods fall short
Standard tissue clearing protocols require laborious solution preparation, often suffer from inconsistent clearing depth, signal loss, and limited fixation compatibility. The FluxMPS™ OmniFix Tissue Clearing Kit eliminates these bottlenecks with a factory-formulated, 0.1 µm ultra-filtered dual-solution system that delivers reproducible optical clarity across all major fixation chemistries — from the first tissue to the hundredth.
Ultra-low Particulate Purity
Filtered through a 0.1 µm membrane (single pass), OmniFix reagents deliver particle counts below the threshold that causes microchannel clogging and optical artifacts in advanced imaging systems.
Maximum Optical Transparency
The EZ View Solution (Solution A) rapidly transforms opaque tissue into optically clear samples, enabling deep-volume confocal, light-sheet, and wide-field fluorescence imaging with exceptional signal-to-noise ratio.
Broad Fixation Compatibility
Validated for EMA, PFA, formalin, ethanol/methanol, and glutaraldehyde-fixed tissues — one kit covers the full spectrum of common histological fixation protocols without reformulation.[1]
Antigen & Fluorophore Preservation
Maintains protein epitopes and fluorescent protein signals throughout the clearing process, supporting downstream immunostaining, antibody labeling, and live-reporter readouts on cleared tissue.[2]
Ready-to-Use Dual-Solution System
Pre-formulated Solution A and Solution B eliminate the preparation errors common in manually mixed clearing reagents. Consistent lot-to-lot formulation ensures reproducibility across experiments.
Customization on Demand
Other concentrations, pH values, chemical additions, or modified protocols are available. Contact support@diagnocine.com with your specifications.
Single-stage 0.1 µm filtration system
The OmniFix Tissue Clearing Kit is DiagnoCine Precision™ filtered-sterilized through a 0.1-micron membrane, once. This single-pass 0.1 µm filtration surpasses the particle-reduction performance of standard 0.22 µm filtered reagents, delivering ultra-low particulate reagents that protect sensitive optical paths and chip geometries. The process is performed in a controlled, non-sterile manufacturing environment under ISO 13485:2016 quality management standards.
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0.1 µmSingle-Pass Membrane Filtration — Final Polish
Both Solution A (EZ View Solution) and Solution B (TF Lipid Removal Solution) are passed through a 0.1 µm membrane filter exactly once. This removes large particulates, aggregates, and sub-micron debris that would otherwise compromise refractive index uniformity and optical clarity during imaging. The 0.1 µm cut-off provides significantly lower particle burden than conventional 0.22 µm filtered laboratory reagents, protecting sensitive detector optics, microfluidic chip geometries, and downstream filter life.
Performance vs. standard 0.22 µm filtered reagents
A single pass through a 0.1 µm membrane captures particle populations that pass freely through 0.22 µm filters. In optical clearing workflows, sub-micron particulates create scattering artifacts that reduce effective imaging depth and resolution. OmniFix reagents, by applying 0.1 µm filtration, deliver measurably lower particle counts per mL — protecting optical paths in light-sheet, confocal, and widefield microscopy systems.
pore size
stage
© Diagnocine® — DCP-OTCK
Optical clearing for next-generation tissue imaging
OmniFix is designed for researchers requiring reliable, deep-volume imaging of fixed tissue samples across diverse biological and pathological workflows. The kit supports immunofluorescence, 3D reconstruction, and multi-modal microscopy on a wide range of tissue types and fixation protocols.
Microfluidic & Chip-Compatible Use
The ultra-low particulate profile of OmniFix reagents (0.1 µm filtered) makes them compatible with microfluidic perfusion systems and organ-on-a-chip (OoC) platforms where particulate accumulation would occlude microchannels or degrade sensor signals. An optional 0.01 µm (10 nm) ultra-filtered variant is available on inquiry for automated bioreactors and robotic perfusion systems.
- Total Particulate Exclusion: 0.1 µm filtration removes all debris above threshold, preventing microchannel clogging in chip-integrated clearing workflows.
- Valve & Sensor Protection: Ultra-low particle counts protect microfluidic valves, pressure sensors, and optical detection elements in automated systems.
- Extended Clearing Stability: Stable reagent chemistry prevents precipitation or viscosity drift that would interrupt continuous-flow clearing protocols.
Inquiry Required: The 0.01 µm (10 nm) ultra-filtered OmniFix grade is available on special request. Contact support@diagnocine.com with your system specifications.
3D Volumetric Tissue Imaging
OmniFix provides the refractive index matching and optical transparency required for undistorted light-sheet illumination throughout thick tissue volumes, enabling whole-organ 3D reconstruction.[3]
Deep-Volume Confocal Imaging
Eliminates light-scattering artifacts to enable confocal z-stack acquisition at depths inaccessible with non-cleared tissues. Compatible with multi-photon excitation and super-resolution modalities.[4]
Antibody Labeling & IF Staining
Preserves antigen accessibility and fluorescent protein integrity throughout the clearing pipeline, supporting reliable immunofluorescence, secondary antibody penetration, and multiplex staining in thick sections.[2]
3D Anatomical Reconstruction
Enables complete 3D rendering of complex tissue architectures including neural circuits, vascular networks, and organoid structures from fixed specimens without physical sectioning artifacts.
Morphology & Pathology Studies
Cleared specimens retain structural features suitable for morphological assessment, disease staging, and cellular architecture analysis in research pathology workflows.[5]
OoC / MPS Tissue Imaging
Ultra-filtered reagents (0.1 µm) are safe for on-chip tissue clearing in organ-on-a-chip (OoC), microphysiological system (MPS), and tissue-on-a-chip (ToC) platforms where particle-free reagents are mandatory.
Analytical & quality specifications
All parameters measured per lot and documented in the Certificate of Analysis. Contact support@diagnocine.com to request a CoA for a specific lot number.
| Parameter | Specification |
|---|---|
| Formulation | Nycodenz, Urea, Sodium Azide, Phosphate Buffer |
| Appearance | Clear-colorless solution |
| pH USP <791> | 7.4 ± 0.04 at 1X |
| RI application | Refractive index matching for optical clearing |
| Protocol role | Step C — RI Matching (24 h incubation) |
| Imaging note | Maintain tissue in EZ View during microscopy |
| Parameter | Specification |
|---|---|
| Formulation | Tetrahydrofuran (THF), Ultrapure Water |
| Appearance | Clear-colorless solution |
| pH USP <791> | 6–7 at 1X |
| THF content | 50% THF in sterile water (working concentration) |
| Protocol role | Step A — Lipid Removal (16 h incubation) |
| Safety | Use in chemical fume hood; chemical waste disposal |
| Parameter | Specification |
|---|---|
| Filtration pore size | 0.1 µm membrane |
| Filtration stages | 1 (single-pass) |
| Fill environment | Non-sterile controlled manufacturing environment |
| Sterility declaration | Filtered 0.1-micron membrane once |
| Manufacturing ISO 13485 | ISO 13485:2016 QMS; CE-approved supplier |
| Water quality (Sol. B) | Ultrapure Water |
| Parameter | Specification |
|---|---|
| Storage temperature | Room Temperature |
| Shelf life — Solution A | 6 months |
| Shelf life — Solution B | 1 month |
| Expiry date | Use before expiry date on product label |
| Post-clearing short-term | Store in EZ View at RT, up to 1 week |
| Post-clearing long-term | Store in EZ View at RT, up to 70 days |
Full composition
The OmniFix Tissue Clearing Kit contains two pre-formulated solutions. All ingredient identities and concentrations are proprietary to DiagnoCine. CAS numbers are provided for informational reference. Nucleic acids from OmniFix-cleared tissues will be fragmented rather than intact — the degree of fragmentation depends on fixation duration and must be optimized per experiment.
| Ingredient | CAS Number | Concentration |
|---|---|---|
| SOLUTION A — EZ VIEW SOLUTION (pH 7.4 ± 0.04) | ||
| Nycodenz | 66108-95-0 | Proprietary |
| Urea | 57-13-6 | Proprietary |
| Sodium Azide | 26628-22-8 | Proprietary |
| Phosphate Buffer | N/A (mixture) | Proprietary |
| Ingredient | CAS Number | Concentration |
|---|---|---|
| SOLUTION B — TF LIPID REMOVAL SOLUTION (pH 6–7) | ||
| Tetrahydrofuran (THF) | 109-99-9 | Proprietary (~50% working) |
| Ultrapure Water | 7732-18-5 | Proprietary |
| Fixation Type | EZ Clear Compatibility | Notes / Considerations |
|---|---|---|
| FIXATION COMPATIBILITY TABLE | ||
| EMA (ethanol–methanol–acetic acid) | High | Original validated use |
| PFA (Paraformaldehyde) | Moderate–High | May need longer clearing; FP preserved |
| Formalin | Moderate | Slower clearing; some opacity may remain |
| Ethanol / Methanol | High | Very compatible, similar to EMA |
| Glutaraldehyde | Low | Strong crosslinking limits clearing |
EMA fixed tissue clearing — Complete EZ Clear Protocol
Full three-step tissue processing sequence for EMA-fixed samples. Adapt incubation times as needed for other fixation types per the compatibility table above.
Step A: Lipid Removal (16 hours)
Using Solution B (TF Lipid Removal Solution) in a chemical fume hood:
- Transfer fixed tissue to glass scintillation vial (20 mL capacity).
- Add 20 mL THF solution (50% THF in sterile water).
- Cover with foil to protect from light.
- Place on orbital shaker in chemical fume hood.
- Incubate 16 hours at room temperature with gentle rocking.
Step B: Washing (4 hours)
- Remove THF solution — dispose in chemical waste container.
- Rinse with sterile Milli-Q water 4 times, 1 hour each.
- Use 20 mL water per wash, gentle rocking.
- Final ventilation — open vial in hood for 10 minutes to remove residual THF.
Step C: Refractive Index Matching (24 hours)
Using Solution A (EZ View Solution):
- Transfer tissue to clean vial or imaging chamber.
- Remove all PBS/water completely.
- Add 5 mL EZ View solution (or sufficient to cover tissue).
- Protect from light with foil wrapping.
- Incubate 24 hours at room temperature with gentle rocking.
- Sample ready for imaging — maintain in EZ View during microscopy.
Troubleshooting Guidelines
Manufacturing standards & compliance
OmniFix Tissue Clearing Kit is manufactured under ISO 13485-certified and CE-approved facility standards. All final packaging, quality assurance, and testing are performed at the DiagnoCine R&D and Quality Testing Center. All customization and assembly are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Manufactured under a full ISO 13485:2016 quality management system with CE-approved supplier status. Products released only after QC testing at DiagnoCine R&D and Quality Testing Center.
0.1 µm Precision Filtration
Both solutions are filtered through a 0.1-micron membrane (single pass) in a controlled manufacturing environment, delivering particle counts below 0.22 µm-filtered media standards.
DiagnoCine Precision Assembly
All final kit assembly, labeling, customization, and quality release performed at DiagnoCine Precision, Totowa, New Jersey, USA under controlled laboratory conditions.
Lot-Specific CoA
Each lot is accompanied by a Certificate of Analysis documenting pH, appearance, filtration grade, and shelf-life validation. Contact support@diagnocine.com for lot-specific CoA.
pH Verification
Solution A: pH 7.4 ± 0.04 at 1X (per lot CoA). Solution B: pH 6–7. Measured by calibrated pH meter per established SOP.
Appearance QC
Both solutions must present as clear-colorless liquid on inspection. Turbidity, color, or particulate contamination constitutes a lot-failure event.
Filtration Verification
0.1 µm membrane filtration applied to every production batch. Filter integrity tested before and after filtration per internal protocol aligned with ISO 13485 requirements.
Documentation & Traceability
Full batch records including raw material traceability, filtration logs, QC results, and CoA maintained. Regulatory documentation available upon request.
How DCP-OTCK compares to conventional clearing methods
OmniFix benchmarked against commonly used manual and commercial tissue clearing approaches to highlight key advantages in purity, compatibility, and workflow convenience.
| Parameter | DCP-OTCK (FluxMPS™ OmniFix) | Manual iDISCO / BABB Method | Generic Commercial Clearing Kit (0.22 µm) |
|---|---|---|---|
| Reagent format | Ready-to-use pre-formulated dual solution | Manual preparation required | Semi-prepared; some mixing required |
| Filtration pore size | 0.1 µm | Typically unfiltered or 0.22 µm | 0.22 µm standard |
| Filtration stages | Single-pass, 0.1 µm membrane | None specified | Single-pass 0.22 µm |
| EMA-fixed tissue compatibility | check_circle High | cancel Not validated | cancel Not validated |
| PFA compatibility | check_circle Moderate–High | check_circle Yes (primary use) | check_circle Yes |
| Formalin compatibility | check_circle Moderate | cancel Limited | cancel Limited |
| Antigen/fluorophore preservation | check_circle Yes | check_circle Variable | check_circle Variable |
| Manufacturing QMS | ISO 13485:2016; CE-approved | No certification | Varies by supplier |
| Custom formulation | check_circle Available on request | cancel No | cancel No |
Frequently asked questions
Common questions about the OmniFix Tissue Clearing Kit (DCP-OTCK).
Supporting literature
Curated peer-reviewed references on tissue optical clearing, lipid-extraction protocols, refractive index matching, and advanced fluorescence microscopy of cleared specimens.
- Erturk A, et al. Three-dimensional imaging of solvent-cleared organs using 3DISCO. Nature Protocols. 2012;7(11):1983–1995.doi:10.1038/nprot.2012.119
- Renier N, et al. iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. Cell. 2014;159(4):896–910.doi:10.1016/j.cell.2014.10.010
- Tomer R, et al. Advanced CLARITY for rapid and high-resolution imaging of intact tissues. Nature Protocols. 2014;9(7):1682–1697.doi:10.1038/nprot.2014.123
- Susaki EA, et al. Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis. Cell. 2014;157(3):726–739.doi:10.1016/j.cell.2014.03.042
- Hama H, et al. Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nature Neuroscience. 2011;14(11):1481–1488.doi:10.1038/nn.2928
- Pan C, et al. Shrinkage-mediated imaging of entire organs and organisms using uDISCO. Nature Methods. 2016;13(10):859–867.doi:10.1038/nmeth.3964
- Zhao S, et al. Cellular and molecular probing of intact human organs. Cell. 2020;180(4):796–812.doi:10.1016/j.cell.2020.01.030
- Ariel P. A beginner's guide to tissue clearing. International Journal of Biochemistry & Cell Biology. 2017;84:35–39.doi:10.1016/j.biocel.2016.12.009
- Marx V. Optimizing probes to image cleared tissue. Nature Methods. 2016;13(3):205–208.doi:10.1038/nmeth.3774
- Vigouroux RJ, Belle M, Chédotal A. Neuroscience in the third dimension: shedding new light on the brain with tissue clearing. Molecular Brain. 2017;10(1):33.doi:10.1186/s13041-017-0314-y
