Sterile: Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
Nonident P-40 (NP-40) is a non-ionic detergent commonly used in cell lysis and protein extraction buffers. This buffer solubilizes membrane proteins without denaturing them, enabling their extraction and purification. The non-ionic nature of NP-40 detergent allows for the extraction of cytoplasmic proteins from both adherent and suspension-cultured mammalian cells without disrupting the native structure and function of the proteins. NP-40 has a high boiling point (~290°C) and a low melting point (< 0°C). The density is around 1.06 g/cm³ at 20°C. Due to its mild, non-denaturing properties, it is suitable for downstream applications like immunoassays (e.g., ELISA, Luminex), Western blotting, and immunoprecipitation. This NP-40 buffer contains glycerol which helps stabilize proteins by preventing denaturation and maintaining their native conformation. Glycerol improves the solubility of proteins, particularly hydrophobic proteins, by creating a more favorable environment for them to remain in solution. The addition of glycerol to lysis buffers can lead to improved protein yields during extraction processes.
Advantages:
The non-denaturing nature of NP-40 helps to maintain protein-protein interactions, enabling the study of protein complexes.
It can selectively lyse the cytoplasmic membrane while leaving the nuclear membrane intact.
Applications:
It is used for solubilizing and extracting membrane proteins and protein complexes without denaturing them.
It enables the study of membrane-associated proteins in their native state.
It is commonly used in cell lysis, immunoprecipitation, and protein purification protocols.
Composition:
Ingredients
Concentration
Tris HCl
20 mM
NaCl
137 mM
Glycerol
10%
NP-40
1%
EDTA
2 mM
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.4
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
RNase activity: No RNase activity was detected after incubating ribosomal RNA with this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: 4℃
Shelf life: 3 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
