/Content/Upload/Product/NO-More_Sale_img_by-Diagncine_1200_199.jpg

No More Sales-Long ssDNA Preparation Kit for 1.5kb, Commercial Entities

Product#: Inactivated-DS615 C

$99,999.00

Qty:

Availability:

Ships in 1-2 Weeks

You are currently tracked by Google's cookies

based on your browsing history record.

We apologize for any inconvenience.

Please, Feel free to Explore or Search

the Diagnocine website for more information.

Let me know if you need further adjustments!

Long ssDNA Preparation Kit for 1.5kb, Commercial Entities

Cat. No. DS615 C
Store RT,-20°C
Size 1 kit

Description

The Long ssDNA(Long Single Strand DNA) Preparation Kits (LsODN Preparation Kits) provide a simple and easy method for generation of a long ssDNA (within 1,500 base or within 3,000 base). A long ssDNA prepared by this kit has defined sequence and length as it does not include inside mutation and terminal deletion caused by PCR, exonuclease side reaction, not high-fidelity reverse transcriptase reaction or not high-fidelity synthetic oligonucleotides.

The procedure is almost same as the method to obtain dsDNA fragments. The DNA of interest is cloned into a plasmid. The resulting plasmid harboring the DNA is digested with a pair of two nicking endonucleases or a combination of a nicking endonuclease and a restriction enzyme. The nicked plasmid is denatured by mixing with Denaturing Gel-Loading Buffer and then subjected to agarose gel electrophoresis. The band corresponding to a long ssDNA is excised and extracted with our kit (#DS640) specially designed for this kit.

Kit Contents

DS615 Long ssDNA Preparation Kit for 1.5 kb (LsODN Preparation Kit)
pLSODN-1 10 µg (0.5 µg/µl)
pLSODN-2D 10 µg (0.5 µg/µl)
Denaturing Gel-Loading Buffer 1 ml (100 loadings)
Long ssDNA Gel Extraction Kit for 3kb (DS640) (25preps)
DS625 Long ssDNA Preparation Kit for 3.0 kb (LsODN Preparation Kit)
pLSODN-3 10 µg (0.5 µg/µl)
pLSODN-4D 10 µg (0.5 µg/µl)
Denaturing Gel-Loading Buffer 1 ml (100 loadings)
Long ssDNA Gel Extraction Kit for 3kb (DS640) (25preps)

Features

A long ssDNA (within 1,500 base or within 3,000 base) can be prepared.
A long ssDNA has defined sequence and length.
Simple principle and easy procedure.
High yield and high quality.

Principle

The DNA of interest is cloned into a plasmid using a pair of two nicking endonuclease sites or a combination of a nicking endonuclease site and a restriction enzyme site.
The resulting plasmid harboring the DNA is digested with a pair of two nicking endonucleases or a combination of a nicking endonuclease and a restriction enzyme.
The nicked plasmid is denatured and then subjected to agarose gel electrophoresis.
The band corresponding to a long ssDNA is excised and extracted.

Plasmid Map

Data

Fig. Long ssDNAs prepared by this kit
A 1.5 kb DNA fragment of interest was cloned between the Nt.BspQI and the Nb.BsrDI sites of pLSODN-1. Similarly, a 3.0 kb DNA fragment of interest was cloned between the Nt.BspQI and the Nb.BsrDI sites of pLSODN-3. The resulting plasmids were digested with Nt.BspQI and Nb.BsrD. The double nicked plasmid was mixed with Denaturing Gel-loading Buffer and heated, then loaded to conventional non-denaturing agarose gel electrophoresis. The band corresponding to a long ssDNA was excised and extracted.

Lane 1: The double nicked pLSODN-1 harboring 1.5 kbp DNA fragment
Lane 2: Purified long ssDNA (1.5 kb)
Lane 3: The double nicked pLSODN-3 harboring 3.0 kbp DNA fragment
Lane 4: Purified long ssDNA (3.0 kb)

Reference