No More Sales - OptiVitro CHO Cell Culture Basal Medium CE01, Serum-free, dry powder (10L)

OptiVitro  CHO Cell Culture Basal Medium CE01, Serum-free, dry powder (10L)  

Cat. No.: EC-CE000-N034
Size: 10L


Description

CHO CE01 Basal Medium SF is an animal origin component-free, protein-free, chemically defined medium. It’s fit for high density culture of CHO-K1, CHO-DG44, CHO-S cells and high level expression of recombinant proteins.


Product Features

• Chemically defined, protein-free, animal-component free
• Support high yield for multiple types of CHO cells
• Enhance productivity and quality
• Stable supply, GMP manufactured
• Provide customized service,optimize your medium to fit workflow needs

Prepare media

1. 1.Measure 80% of the final volume WFI or distilled water in a clean vessel.
2. 2.Add 22.15g/L CHO CE01 Basal Medium SF powder slowly to the vessel, mix for 30 minutes.
3. 3.Adjust the pH to 8.5 with 5 N NaOH solution. After adjusting, continue stirring for an additional 10 minutes.
4. 4.Adjust the pH to 7.0 with 6 N HCl solution. After adjusting, continue stirring for an additional 5 minutes.
5. 5.Add 2.317g/L NaHCO3 and mix for 10 minutes.
6. 6.QS to final production volume and mix for 5 minutes.
7. 7.Measure the pH and osmolality. pH should be 6.90 to 7.10. Osmolality should be 270 to 300 mOsm/kg.
8. 8.Sterilize immediately by membrane filtration (< 0.22 microns), and store at 2 to 8°C in the dark until use.

 
Product Data

Batch culture

Figure 1. Cell viability , density and IgG titer of OptiVitro® CE01 and Medium 1 cultured in batch culture for 6 days Cell line: CHO-K1

Fed-batch culture

Figure 2. OptiVitro® CE01 matched with feed CA01 can compare favourably with world-class medium, supports persistent cell survival and high level expression of mAb（9.047 g/L). Cell line: CHO-K1.


Figure 3. OptiVitro® CE01 matched with feed CA01 can compare favourably with world-class medium,supports persistent cell survival and high level expression of BsAb（2.4 g/L). Cell line: CHO-K1.



Product Information
 

Product ID	Product	Storage	Size
EC-CE000-N033	OptiVitro  CHO Cell Culture Basal Medium CE01, Serum-free, dry powder	2-8°C, Store dark	1L
EC-CE000-N034	OptiVitro  CHO Cell Culture Basal Medium CE01, Serum-free, dry powder	2-8°C, Store dark	10L
EC-CE000-N035	OptiVitro  CHO Cell Culture Basal Medium CE01, Serum-free, dry powder	2-8°C, Store dark	100L