Native Loading Buffer [5X]
Cat No.: DCP-NLB5X
Size: 20 ml
Sterile; Ultrapure, Filtered 1-micron membrane once and 0.45-micron membrane ONCE
Description:
Native loading buffer, also known as native sample buffer, is a specialized reagent used in protein electrophoresis to prepare samples for analysis under non-denaturing conditions. The native conditions allow enzymes and other proteins to maintain their biological activity, which can be assessed after electrophoresis. In native PAGE, proteins migrate based on their native mass-to-charge ratio, allowing separation of proteins with the same size but different shapes or charges. It allows loading of samples into gel wells and provides visual tracking of sample migration during electrophoresis. Native loading buffers do not contain denaturing agents like SDS or reducing agents, allowing proteins to retain their native conformation, oligomeric states, and protein-protein interactions during electrophoresis.
Note: It cannot be used for molecular weight estimation like SDS-PAGE.
Applications
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Used for native PAGE to separate proteins based on size, shape, and charge
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Retains biological activity and protein-protein interactions
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Allows separation of proteins with the same size but different shapes/charges
Advantages:
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Preserves native protein structure and oligomeric states.
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Maintains protein-protein interactions.
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Allows analysis of protein complexes.
Composition:
| Ingredients |
Concentration |
| Tris-HCl |
250 mM |
| Glycerol |
40% |
| Bromophenol Blue |
0.01% |
Quality Control:
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Appearance: Blue
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pH: 6.8
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Sterility: Filtered 1-micron membrane once and 0.45-micron membrane ONCE in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
