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Mouse Vasodilator-stimulated Phosphoprotein, VASP ELISA Kit
Product#: BTL-E2560Mo
$686.40
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Mouse Vasodilator-stimulated Phosphoprotein, VASP ELISA Kit
Cat. No#: BTL-E2560Mo
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 1.58ng/L
Detection range: 3-900ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Mouse
Product overview
Full product name
Vasodilator-stimulated phosphoprotein
SKU#.: ? BTL- |
E2560Mo |
Assay type |
Sandwich |
Size |
1.58ng/L |
Detection range |
3-900ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Mouse |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation (By similarity). |
UniProt accession |
39,667 |
GeneID |
22323 |
Synonyms |
Vasodilator-stimulated phosphoprotein;VASP |
Gene names |
Vasp |
Research area |
Signal Transduction; Neuroscience; Cell Biology; Cardiovascular; Metabolism |
Target protein |
VASP |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2560Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/L | 1.96 | 1.957 | 1.908 |
| 1.954 | |||
| 240ng/L | 1.134 | 1.045 | 0.996 |
| 0.955 | |||
| 120ng/L | 0.417 | 0.448 | 0.399 |
| 0.48 | |||
| 60ng/L | 0.226 | 0.247 | 0.198 |
| 0.269 | |||
| 30ng/L | 0.099 | 0.117 | 0.068 |
| 0.136 | |||
| 0ng/L | 0.057 | 0.049 | 0 |
| 0.041 |
Precision
We measured random samples of E2560Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 111.03 | 5.14 | 4.63 |
| Intra-Assay | 2 | 18 | 323.18 | 9.72 | 3.01 |
| Intra-Assay | 3 | 18 | 218.72 | 7.89 | 3.61 |