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Mouse Thrombopoietin, TPO ELISA Kit
Product#: BTL-E0113Mo
$686.40
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Mouse Thrombopoietin, TPO ELISA Kit
Cat. No#: BTL-E0113Mo
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 1.54ng/L
Detection range: 3-900ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Mouse
Product overview
Full product name
Thrombopoietin
SKU#.: ? BTL- |
E0113Mo |
Assay type |
Sandwich |
Size |
1.54ng/L |
Detection range |
3-900ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Mouse |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. |
UniProt accession |
37,836 |
GeneID |
21832 |
Synonyms |
C-mpl ligand;Megakaryocyte colony-stimulating factor;Megakaryocyte growth and development factor;MGDF;ML;Myeloproliferative leukemia virus oncogene ligand;THPO;Thrombopoietin |
Gene names |
THPO |
Research area |
Cardiovascular |
Target protein |
THPO |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0113Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/L | 2.308 | 2.208 | 2.133 |
| 2.109 | |||
| 240ng/L | 1.432 | 1.449 | 1.373 |
| 1.466 | |||
| 120ng/L | 0.702 | 0.688 | 0.612 |
| 0.674 | |||
| 60ng/L | 0.436 | 0.465 | 0.389 |
| 0.494 | |||
| 30ng/L | 0.246 | 0.255 | 0.179 |
| 0.265 | |||
| 0ng/L | 0.078 | 0.076 | 0 |
| 0.073 |
Precision
We measured random samples of E0113Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 139.9 | 7.04 | 5 |
| Intra-Assay | 2 | 18 | 257.6 | 7.4 | 2.9 |
| Intra-Assay | 3 | 18 | 118.4 | 5.4 | 4.6 |