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Mouse Shadow of Prion Protein, SPRN ELISA Kit
Product#: BTL-E2468Mo
$686.40
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Mouse Shadow of Prion Protein, SPRN ELISA Kit
Cat. No#: BTL-E2468Mo
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.025ng/mL
Detection range: 0.05-20ng/mL
Sample type: Serum, plasma, cell culture supernates
Reactive with: Mouse
Product overview
Full product name
Shadow of prion protein
SKU#.: ? BTL- |
E2468Mo |
Assay type |
Sandwich |
Size |
0.025ng/mL |
Detection range |
0.05-20ng/mL |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Mouse |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Prion-like protein that has PrP(C)-like neuroprotective activity. May act as a modulator for the biological actions of normal and abnormal PrP. |
UniProt accession |
14,591 |
GeneID |
212518 |
Synonyms |
C630041J07;Protein shadoo;sh;Shadow of prion protein;SPRN |
Gene names |
Sprn |
Research area |
Neuroscience |
Target protein |
SPRN |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2468Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/mL | 2.417 | 2.35 | 2.296 |
| 2.284 | |||
| 6ng/mL | 1.348 | 1.388 | 1.334 |
| 1.427 | |||
| 3ng/mL | 0.839 | 0.807 | 0.753 |
| 0.775 | |||
| 1.5ng/mL | 0.434 | 0.438 | 0.385 |
| 0.443 | |||
| 0.75ng/mL | 0.307 | 0.293 | 0.239 |
| 0.279 | |||
| 0ng/mL | 0.054 | 0.054 | 0 |
| 0.053 |
Precision
We measured random samples of E2468Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 8.44 | 0.3 | 3.5 |
| Intra-Assay | 2 | 18 | 2.82 | 0.13 | 4.46 |
| Intra-Assay | 3 | 18 | 1.96 | 0.09 | 4.46 |
