DiagnoCine Precision® ELISA Kit Reagents included
- SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
- Universal Blocking Buffer: use when background interference compromises signal-to-noise ratio
- Protease Inhibitor Cocktail: incorporate during the sample preparation phase or add directly to the ELISA protocol as required to prevent protein degradation.
Exclusive additions: All kits come with three DiagnoCine Precision® reagents for improving the effectiveness of your testing and clarity of your results
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Mouse Prion Protein (PrPc)
Cat.No.: JNB-JL52328
Size: 96T
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.077ng/mL
Detection Range: 0.156-10ng/mL
Alternative Names: CD230; GSS; ASCR; CJD; PRIP; PrP; PrP2730;Prnp;Prn;AltPrP;Major prion protein; Creutzfeldt-Jakob Disease; Gerstmann-Strausler-Scheinker Syndrome; Fatal Familial Insomnia;Alternative prion protein; major prion protein
Research Areas: Signal Transduction
Assay Time: 2h45m
Abbreviation: PrPc
Test Principle: This assay employs the sandwich enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PrPc. Add Sample, Standard, Biotin-antibody, and Streptavidin-HRP by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is positive to the Mouse PrPc in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Mouse PrPc in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Mouse PrPc in samples. No significant cross-reactivity or interference between Mouse PrPc and analogues was observed.
Recovery rate: 80%-120%
Precision: Intra-CV and Inter-CV <10%
Shipping: Ice Bag
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