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Mouse Fatty Acid Synthase, FASN ELISA Kit
Product#: BTL-E4250Mo
$686.40
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Mouse Fatty Acid Synthase, FASN ELISA Kit
Cat. No#: BTL-E4250Mo
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.57ng/L
Detection range: 1-400ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Mouse
Product overview
Full product name
Fatty Acid Synthase
SKU#.: ? BTL- |
E4250Mo |
Assay type |
Sandwich |
Size |
0.57ng/L |
Detection range |
1-400ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Mouse |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain. |
UniProt accession |
272,428 |
GeneID |
14104 |
Synonyms |
[Acyl-carrier-protein] S-malonyltransferase;3-hydroxyacyl-[acyl-carrier-protein] dehydratase;3-oxoacyl-[acyl-carrier-protein] reductase;3-oxoacyl-[acyl-carrier-protein] Synthase;Acyl-[acyl-carrier-protein] hydrolase;Enoyl-[acyl-carrier-protein] reductase;FASN;Fatty acid Synthase;Type I Fatty Acid Synthase [Includes: [Acyl-carrier-protein] S-acetyltransferase; |
Gene names |
FASN |
Research area |
Metabolism |
Target protein |
FASN |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4250Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 240ng/L | 2.347 | 2.232 | 2.186 |
| 2.117 | |||
| 120ng/L | 1.703 | 1.704 | 1.658 |
| 1.705 | |||
| 60ng/L | 1.259 | 1.209 | 1.163 |
| 1.158 | |||
| 30ng/L | 0.637 | 0.645 | 0.599 |
| 0.652 | |||
| 15ng/L | 0.414 | 0.391 | 0.345 |
| 0.368 | |||
| 0ng/L | 0.056 | 0.046 | 0 |
| 0.036 |
Precision
We measured random samples of E4250Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 44.2 | 2.31 | 5.2 |
| Intra-Assay | 2 | 18 | 73.9 | 3.65 | 4.9 |
| Intra-Assay | 3 | 18 | 15 | 0.65 | 4.4 |