.jpg "/Content/Upload/Product/Mouse_ELISA_kits_Diagnocine_1200(11225).jpg")
Mouse Dehydroepiandrosterone (DHEA)
Product#: JNB-JL12271
$614.40
Availability:
Ships in 24 hours
DiagnoCine Precision® ELISA Kit Reagents included
- SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
- Universal Blocking Buffer: use when background interference compromises signal-to-noise ratio
- Protease Inhibitor Cocktail: incorporate during the sample preparation phase or add directly to the ELISA protocol as required to prevent protein degradation.
Exclusive additions: All kits come with three DiagnoCine Precision® reagents for improving the effectiveness of your testing and clarity of your results
All ELISA kit types available—including special MOQ pricing! Need 48T or other Questions?Contact us anytime—we’re happy to help! support@diagnocine.com
Mouse Dehydroepiandrosterone (DHEA)
Cat.No.: JNB-JL12271
Size: 96T
Species: Mouse
Assay Type: Competitive
Sensitivity: 0.39ng/mL
Detection Range: 0.78-50ng/mL
Alternative Names: 3β-Hydroxyandrost-5-en-17-one;Androstenolone;Prasterone;Androst-5-en-3β-ol-17-one; 5,6-Didehydroepiandrosterone;Dehydroisoepiandrosterone
Research Areas: Neuroscience
Assay Time: 1h45m
Abbreviation: DHEA
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with Mouse DHEA antigen. Add Sample, Standard, Biotin-antibody, and Streptavidin-HRP by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is opposite to the Mouse DHEA in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Mouse DHEA in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Mouse DHEA in samples. No significant cross-reactivity or interference between Mouse DHEA and analogues was observed.
Recovery rate: 80%-120%
Precision: Intra-CV and Inter-CV <10%
Shipping: Ice Bag
