Monoclonal Antibody Against Human Blood Group Lea (4620)
Product#: FNK-2AHBGLA
$260.92
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Monoclonal Antibody Against Human Blood Group Lea (4620)
| Cat. No: | FNK-2AHBGLA |
| Clone No: | Hyb-4620 |
| Manufacturing Process: | Hybridoma obtained by fusing mouse myeloma cells (NS-1) with BALB/c mouse spleen cells immunized with blood group substance derived from human saliva is proliferated in the BALB/c mouse abdominal cavity and ascites is collected. |
| Specificity: | This monoclonal antibody specifically agglutinates human blood group Le(a+b-) red blood cells (coombs test). |
| Purification: | Ammonium sulfate precipitation. |
| Ig Class: | IgG1 |
| Form: | Saline (containing 0.1% NaN3) |
| Protein Concentration: | 0.5 mg/mL |
| Package: | 0.2 mL |
| Storage: | 2-8℃ (Below -20℃ for long term storage) |
| Shipping Condition: | Dry Ice |
| Application: | Hemagglutination |
Antigen Characterization
- Type: Primary
- Application: ELISA, Western Blot
- Species: Human
- Recombinant: Yes
Human Diseases
- Bombay Phenotype (hh): Individuals with this phenotype lack the H antigen, which is necessary for the formation of Lewis antigens, leading to potential complications in blood transfusions and organ transplants.
- Gastrointestinal Infections: Lewis antigens, including Lea, have been associated with susceptibility to infections such as Helicobacter pylori, which can lead to gastric diseases.
- Transfusion Reactions: Mismatched blood transfusions involving Lewis antigens can lead to adverse reactions, particularly in individuals with anti-Lea antibodies.
- Cancer: Altered expression of Lewis antigens has been linked to tumor progression and metastasis in various cancers, where they may influence immune evasion and cell adhesion.
Cellular Signaling Pathways
- Cell Adhesion and Migration: The Lea antigen is involved in cell-cell interactions and adhesion processes, influencing leukocyte migration during inflammatory responses.
- Immune Response Modulation: The presence of Lea can affect T cell activation and differentiation, thereby modulating overall immune responses.
- Interaction with Pathogens: Lewis antigens may serve as receptors for certain pathogens, influencing their ability to adhere to host tissues and evade immune detection.
- Glycosylation Pathways: The synthesis of Lea involves specific glycosyltransferases that add fucose residues to oligosaccharides, impacting various cellular functions related to glycosylation.
Aliases for Lea (Lewis a) Gene
- Lewis A antigen (Lea)
- Le(a) antigen
- Lewis blood group antigen A
- Le(a) glycoprotein
References
- WF;, Dunstan RA;Simpson MB;Rosse. “Lea Blood Group Antigen on Human Platelets.” American Journal of Clinical Pathology, U.S. National Library of Medicine, pubmed.ncbi.nlm.nih.gov/3966446/.
- Subramaniyan, Rajeswari. “Serological Characteristics of Lewis Antibodies and Their Clinical Significance - a Case Series.” Hematology, Transfusion and Cell Therapy, U.S. National Library of Medicine, 2023, www.ncbi.nlm.nih.gov/pmc/articles/PMC10244237/.
- "Lewis System: Anti-Lewis.” Professional Education, 30 Jan. 2023, professionaleducation.blood.ca/en/transfusion/best-practices/serological-best-practices/lewis-system-anti-lewis.
- “The Lewis Blood Group System and Secretor Status.” Biomedical Scientist, 23 Jan. 2024, thebiomedicalscientist.net/2019/01/07/lewis-blood-group-system-and-secretor-status.
- Dean L. Blood Groups and Red Cell Antigens [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2005. Chapter 6, The Hh blood group. Available from: https://www.ncbi.nlm.nih.gov/books/NBK2268/
- Alhajj M, Zubair M, Farhana A. Enzyme Linked Immunosorbent Assay. [Updated 2023 Apr 23]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK555922/
- Ewald, D. R., & Sumner, S. C. (2016). Blood type biochemistry and human disease. Wiley interdisciplinary reviews. Systems biology and medicine, 8(6), 517–535. https://doi.org/10.1002/wsbm.1355
