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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-MEM-PBN1X
$34.10
DCP-MEM-PBN1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose (Low, 1.0 g/L) Without Sodium Bicarbonate Without HEPES Without Sodium Pyruvate

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Quadruple-stage filtration train: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II) reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (USP <85> BET), controlled per manufacturing batch
  • Low-glucose MEM base (1.0 g/L D-Glucose); formulated without sodium pyruvate and without sodium bicarbonate; pH 7.4 (USP <791>)
  • Phenol red included (11 mg/L) for visual pH monitoring; L-glutamine supplied at 292 mg/L
  • Manufactured under an ISO 13485:2016 quality management system with aseptic fill & finish in Totowa, NJ
  • Mycoplasma risk managed via 0.1 µm mycoplasma-retentive final-stage filtration (not tested per lot)
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-MEM-PBN1X |Sizes: 500 mL, 1000 mL |Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L)
  • L-Glutamine292 mg/L
  • Sodium PyruvateNot added
  • HEPESNot added
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings, triggering inflammation in primary cultures, and shortening device lifetimes. FluxMPS™ is built to address these failure modes at the filtration stage.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.

target

Total metabolic control

Defined low-glucose carbon source without sodium pyruvate gives researchers full control over energy substrate availability in metabolic flux experiments.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696), supporting low trace-metal and organic-carbon (TOC) background for sensitive assay systems.

visibility

Low background for imaging

Low particulate baseline supports confocal microscopy, live-cell biosensors, and TEER measurements with reduced contaminant interference.

science

Rich, stable nutrient profile

Micro-batch precision locks in amino acid and vitamin concentrations for lot-to-lot reproducibility in long-term perfusion studies.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is processed through a four-pass filtration train — two dedicated 0.1 µm/0.04 µm prefilter-and-final-filter pairs run in series — reaching a 0.04 µm final cut-off that addresses particulate classes a single 0.22 µm pass does not retain.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge and extends filter life.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates, including mycoplasma-sized organisms (0.2–0.3 µm), that pass through a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge and providing full-train redundancy against upstream breakthrough.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish performed in an ISO Class 5 laminar-flow workstation under ISO 13485-aligned conditions.

Performance vs. conventional media

Four sequential stages reaching 0.04 µm deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every lot.

5×
 
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is controlled through 0.1 µm mycoplasma-retentive filtration at the final-stage pair (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate 1X Liquid (DCP-MEM-PBN1X) Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEM-PBN1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid is intended for organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant of this formulation is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion. This is a distinct filtration tier from the 0.04 µm Microfluidics Suitable product described on this page.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade version.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation prevents microchannel clogging and maintains laminar flow integrity.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Low-glucose base with a defined carbon source supports precise metabolic flux and Warburg-effect studies.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin variability causes off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurement stability.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

Defined low-glucose base without sodium pyruvate supports isotope-tracing metabolic studies. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate load supports confocal imaging and biosensor integration workflows.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation Contains L-Glutamine, Phenol Red, Calcium, Magnesium, Glucose (low, 1.0 g/L); without Sodium Bicarbonate, HEPES, Sodium Pyruvate
Appearance Orange-to-red colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 1000 mg/L (1.0 g/L)
L-Glutamine 292 mg/L
Sodium Pyruvate Not added
Phenol Red 11 mg/L (present)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement Bicarbonate-free as supplied; CO2 incubation and/or buffer supplementation (sodium bicarbonate or HEPES) should be selected per your culture system
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Pack sizes 500 mL, 1000 mL
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.00
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30189-89-0 31.300
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-45-6 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 11.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water (ASTM D1193 Type I / ISO 3696).

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing ensures lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay on every manufacturing batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result: Contact for specification (see Certificate of Analysis).

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com with your lot number.
Product Comparison

How DCP-MEM-PBN1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter DCP-MEM-PBN1X (FluxMPS™) Conventional MEM (0.22 µm) Standard MEM alternative
Grade Microfluidics Suitable 0.22 µm filtered (standard grade) 0.22 µm filtered (standard grade)
Base Formulation MEM, Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid MEM Standard MEM Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-MEM-PBN1X is Microfluidics Suitable, processed through our Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2), delivering ultra-low particulate counts that reduce microchannel clogging risk in OoC and MPS devices.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm) or many subvisible particulates that accumulate in microchannels. FluxMPS™ uses four sequential stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788> Method 1.
This MEM base is supplied without sodium pyruvate (an alternative energy substrate for some cell lines) and without sodium bicarbonate (the standard CO2-dependent buffer). Sodium pyruvate can be added at the time of use for pyruvate-dependent cell lines, and either sodium bicarbonate or HEPES can be added depending on your incubation environment. Contact support@diagnocine.com for a custom pre-formulated version.
This formulation is supplied without sodium bicarbonate and without HEPES. CO2 incubation and buffering approach should be selected based on your culture system — add sodium bicarbonate for standard CO2 incubation, or add HEPES for open-air / non-CO2 conditions.
Yes. FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid can be supplemented with FBS (5–20%), growth factors, antibiotics, or other additives per standard practice. Filter serum and protein-containing supplements through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm membrane for serum, as it will strip serum of functional protein and lipoprotein content. Add supplements immediately before use.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> BET (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. Batch-specific results are reported on the Certificate of Analysis, available from support@diagnocine.com.
Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, lot number, expiry, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  5. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  6. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  7. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  8. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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