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FluxMPS™ MEM Low Glucose with Earle's Salts w/o Sodium Pyruvate

Product#: DCP-MEM-PN1X
$34.10
DCP-MEM-PN1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ MEM Low Glucose with Earle's Salts w/o Sodium Pyruvate

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Without HEPES Without Sodium Pyruvate

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm x2 + 0.04 µm x2) 1X liquid Minimum Essential Medium formulated with Earle's salts, low glucose, L-glutamine, and sodium bicarbonate — without sodium pyruvate — for precise carbon-source control in microfluidic systems, organ-on-a-chip platforms, and sensitive mammalian cell culture.

  • Quadruple-stage filtration: 0.1 µm x2 passes + 0.04 µm x2 passes — Microfluidics Suitable, microchannel-safe purity
  • Endotoxin release specification: less than 0.05 EU/mL (USP <85> BET), tested per manufacturing batch
  • Low-glucose formulation (1.0 g/L D-glucose) with Earle's salts for pH and osmotic balance; formulated without sodium pyruvate
  • Contains L-glutamine (292.0 mg/L), sodium bicarbonate (2200 mg/L), and phenol red indicator (11.0 mg/L); formulated without HEPES
  • Manufactured under an ISO 13485:2016 quality management system with ISO Class 5 (Class 100) aseptic fill; final QC at Diagnocine Precision, Totowa, NJ
  • Sterility per USP <71> — no bacterial or fungal growth observed after 14 days of incubation
  • Custom pH, glucose concentration, salts, HEPES, and nutrient composition available on request — contact support@diagnocine.com
DCP-MEM-PN1X |Size: 500 mL and 1000 mL |Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
FluxMPS™ MEM Low Glucose with Earle's Salts w/o Sodium Pyruvate · Liquid 1X
  • Glucose1.0 g/L (Low)
  • L-Glutamine292.0 mg/L (Included)
  • Sodium PyruvateNot included
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)265–305 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm x2 + 0.04 µm x2 (Quadruple-stage)
  • Storage2–8°C, protected from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingAmbient (cold pack on request)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered MEM-grade media leaves behind subvisible particulates and mycoplasma-sized organisms that clog microchannels, corrupt metabolic assay signals, and undermine reproducibility in organ-on-a-chip and high-sensitivity cell culture systems. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

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Microchannel-Safe Purity

Final 0.04 µm filtration is compliant with USP <788> Method 1 (light obscuration) particulate thresholds — helps prevent aggregate accumulation that can block microfluidic channels and distort flow profiles.

target

Total Metabolic Control

Low-glucose (1.0 g/L) Earle's salt formulation without sodium pyruvate enables precise Warburg-effect studies and carbon-source-defined metabolic experiments free from pyruvate interference.

science

Sub-Mycoplasma Filtration Stages

The 0.1 µm and 0.04 µm membrane stages are sized below the diameter of the smallest mycoplasma species (0.2–0.3 µm), providing mycoplasma-retentive filtration that is not tested per lot.

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Ultrapure Type 1 Water

Formulated with 18.2 MΩ·cm ultrapure Type 1 water — supporting low trace-metal and organic-carbon background for sensitive formulation work.

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Regulatory-Grade QMS

Manufactured under an ISO 13485:2016 quality management system, 21 CFR Part 820 (QMSR) aligned. Every lot released against endotoxin, sterility, pH, osmolality, and cultural-response testing.

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Customization on Demand

pH, glucose concentration, Earle's vs. Hank's salts, HEPES addition, and nutrient modifications available on request — support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ MEM is a Microfluidics Suitable, ready-to-use 0.04 µm-polished MEM. Each lot passes through four sequential filtration stages, run as two dedicated prefilter-plus-final-filter pairs, in an ISO Class 5 (Class 100) aseptic fill environment — delivering validated sterility and ultra-low particulate burden for demanding microfluidic and high-sensitivity cell culture applications.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates and aggregates from raw formulation, and protects the first 0.04 µm final-filter cartridge downstream.

  2. 2

    0.04 µm Final Filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter that protects the second 0.04 µm final-filter cartridge — this is not polishing the first filter's effluent, it is a fresh prefilter for the second pair.

  4. 4

    0.04 µm Final Filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill and finish in an ISO Class 5 (Class 100) environment — delivers the lowest achievable particulate count for this liquid MEM.

Performance vs. conventional media

Conventional 0.22 µm-filtered MEM allows passage of subvisible particles and mycoplasma-sized organisms that can accumulate in microfluidic channels, interfere with optical biosensors, and add background to metabolic flux measurements. The 0.04 µm final filter in FluxMPS™ MEM delivers a substantially cleaner particulate baseline for sensitivity-critical experiments.

5x
Cleaner by particulate count vs. 0.22 µm-filtered media
0.04 µm
Final filter pore size across 4 filtration passes
Sterility & Mycoplasma Control: No bacterial or fungal growth observed after 14 days of incubation per USP <71> specification. Mycoplasma control is achieved through the 0.1 µm and 0.04 µm filtration stages, which are sized below the diameter of the smallest mycoplasma species (0.2–0.3 µm); mycoplasma is not tested per lot.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ MEM Low Glucose with Earle's Salts w/o Sodium Pyruvate (DCP-MEM-PN1X) Quadruple-stage filtration system diagram ? 0.1 μm x2 + 0.04 μm x2 ultra-filtration for organ-on-a-chip and microfluidic cell culture media ? Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system — four progressive membrane stages (0.1 µm x2 + 0.04 µm x2) for Microfluidics Suitable purity.
© Diagnocine® — DCP-MEM-PN1X
Applications

Where FluxMPS™ MEM performs

FluxMPS™ MEM Low Glucose with Earle's Salts supports research applications that demand both precise low-glucose metabolic control and Microfluidics Suitable purity. From microfluidic organ-on-a-chip platforms to Warburg-effect metabolomics, this formulation supports consistent, reproducible results across sensitive biological systems.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of FluxMPS™ MEM is available for automated bioreactor and liquid-handling robotics applications where even 0.04 µm-level particulates may risk valve fouling, sensor drift, or perfusion inconsistency over extended culture runs.

  • Total Particulate Exclusion: 10 nm filtration removes nanoscale aggregates incompatible with precision fluidic systems
  • Valve & Sensor Protection: Helps reduce particulate-driven fouling in microvalves, pressure transducers, and optical sensors
  • Extended Perfusion Stability: Ultra-clean media supply intended for multi-day perfusion culture

Inquiry Required: The 0.01 µm (10 nm) MPS Grade variant is produced in small batches and is not listed as a standard catalog item. Contact support@diagnocine.com to request this variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Microfluidics Suitable, low-particulate MEM for organ-on-a-chip and tissue-chip platforms requiring particulate-controlled media compatible with PDMS and glass microchannel surfaces.

OoC ToC BoC LoC MPS
Cancer Biology

Warburg Effect & Metabolic Research

Low-glucose, pyruvate-free formulation supports isolating glycolytic flux from oxidative phosphorylation — enables Warburg-effect modeling without confounding exogenous carbon sources.

MCF-7 MDA-MB-231 HeLa A549
Stem Cell Biology

iPSC-Derived Models

Earle's salt formulation supports iPSC-derived cell types sensitive to osmotic fluctuation and metabolic perturbation during differentiation and functional assay phases.

iPSC-Neurons iPSC-CM iPSC-Hep
Vascular Biology

Endothelial & Primary Cells

MEM with Earle's salts supports primary endothelial and hepatocyte cultures, and is broadly used for fibroblast culture, virus propagation, HeLa, BHK-21, 293, HEp-2, HT-1080, and MCF-7 applications.

HUVECs HAECs Primary hepatocytes
Metabolomics

Metabolic Flux Analysis

Pyruvate-free, defined low-glucose base medium supports isotope-tracing metabolic flux experiments with a clearly defined carbon source. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracing NMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate burden helps minimize particulate-driven background scatter in live-cell imaging, biosensor, and TEER measurement setups.

Confocal Biosensors TEER
Technical Specifications

Lot-release analytical parameters

Every lot of FluxMPS™ MEM Low Glucose with Earle's Salts is released against the following analytical criteria. Values represent specification limits; Certificates of Analysis with lot-specific measured values are available on request. Available pack sizes: 500 mL, 1000 mL.

Physical & Chemical Parameters
Parameter Specification
Formulation MEM with Earle's Salts — [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [-] HEPES, [-] Sodium Pyruvate
Appearance Orange-to-red, clear solution (phenol red indicator)
pH USP <791> 7.4
Osmolality USP <785> 265–305 mOsm/kg H2O
D-Glucose 1,000 mg/L (1.0 g/L)
L-Glutamine 292.0 mg/L
Sodium Pyruvate Not present
Phenol Red 11.0 mg/L (sodium salt)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm and 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥ 10 µm USP <788> Method 1 Compliant
Particulate ≥ 25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1 (18.2 MΩ·cm)
Manufacturing Std. ISO 13485:2016 certified supplier
Fill Environment ISO Class 5 (Class 100) aseptic
Storage, Handling & Logistics
Parameter Specification
Storage Temperature 2–8°C, protected from light
Freeze-Thaw Do not freeze
Shelf Life 12 months from date of manufacture, unopened
Shipping Condition Ambient (cold pack on request)
CO2 Requirement Approximately 5% CO2 atmosphere recommended (consistent with Earle's salts sodium bicarbonate buffering at 2200 mg/L)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw Material Grade Cell culture / reagent grade or higher
Traceability Full lot-level documentation; CoA available on request
Manufacturing QMS ISO 13485 ISO 13485:2016 certified supplier
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory Alignment 21 CFR Part 820 (QMSR) aligned; USP <85>, <71>, <785>, <788>, <791>
Production Method Compounding + quadruple-stage membrane filtration; ISO Class 5 aseptic fill
Intended Use Research Use Only (RUO). Not for clinical, diagnostic, or therapeutic use.
Formulation

Full composition (mg/L)

Complete formulation at 1X concentration. Total: 29 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others). Customization of any component is available on request — contact support@diagnocine.com.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 1007-42-7 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-55-8 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 11.000
Customization Available: Other glucose concentrations, addition of sodium pyruvate, HEPES, NEAA, calcium-free formulation (for suspension cultures), modified pH, or alternative salt compositions are available. Please contact us for customization options.
Quality Assurance

Manufacturing standards & compliance

FluxMPS™ MEM is produced under an ISO 13485:2016-certified quality management system, with final packaging, quality assurance, and testing performed at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering design controls, supplier qualification, production, and post-market surveillance — 21 CFR Part 820 (QMSR) aligned.

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Quadruple-Stage Filtration

0.1 µm x2 + 0.04 µm x2 filtration train delivers Microfluidics Suitable purity at every lot.

biotech

ISO Class 5 Fill & Finish

Final aseptic fill performed in an ISO Class 5 (Class 100) cleanroom environment.

assignment

Per-Lot QC & CoA

Small-batch compounding with full lot traceability — each batch is tested for pH, osmolality, endotoxin, sterility, and cultural response before release.

Endotoxin — USP <85> BET

LAL assay per USP <85>; assay sensitivity 0.005 EU/mL. Release specification: less than 0.05 EU/mL.

Particulate Matter — USP <788> Method 1

Light obscuration particle count testing per USP <788> Method 1 — confirms low particulate burden consistent with Microfluidics Suitable media.

Osmolality — USP <785>

Freezing-point depression osmometry confirms 265–305 mOsm/kg H2O for the finished formulation.

Documentation / CoA

Certificate of Analysis with lot number, expiry date, and measured release results available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request. Contact support@diagnocine.com.
Product Comparison

How DCP-MEM-PN1X compares

Standard 0.22 µm-filtered MEM formulations leave subvisible particulates and mycoplasma-sized organisms that can accumulate in sensitive systems. FluxMPS™ MEM addresses these gaps with a validated four-stage filtration architecture.

Parameter DCP-MEM-PN1X (FluxMPS™) Conventional MEM (0.22 µm-filtered)
Grade Microfluidics Suitable (0.04 µm final) Not specified
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [-] HEPES, [-] Sodium Pyruvate Varies by supplier
Final filtration pore size 0.04 µm 0.22 µm
Number of filtration stages 4 (0.1 µm x2 + 0.04 µm x2) 1 stage
Mycoplasma-retentive filtration check_circle 0.1/0.04 µm stages (not tested per lot) cancel Not addressed
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Method 1, compliant cancel Not specified
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Not specified
Manufacturing QMS ISO 13485:2016 certified Varies
Microfluidic channel compatibility check_circle Validated cancel Not validated
Custom formulation check_circle pH, salts, supplements, glucose cancel Fixed formulation

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ MEM Low Glucose with Earle's Salts w/o Sodium Pyruvate.

Yes. FluxMPS™ MEM Low Glucose with Earle's Salts is engineered for microfluidic applications including organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological systems (MPS). The 0.04 µm final filter removes particulates that would otherwise accumulate in narrow microchannels, foul valves, and scatter light in optical biosensor setups, and helps preserve TEER measurements in epithelial and endothelial barrier models. Beyond chip-based systems, this formulation is also broadly used for adherent lines including HeLa, BHK-21, 293, HEp-2, HT-1080, MCF-7, fibroblasts, and primary endothelial and astrocyte cultures; supplementation with fetal bovine serum (FBS) or an equivalent is typically required since the medium contains no protein, lipid, or growth-factor components.
Conventional MEM is typically filtered once through a 0.22 µm membrane, which allows subvisible particulates and mycoplasma-sized organisms (0.2–0.3 µm) to pass through. FluxMPS™ MEM instead runs a quadruple-stage train — 0.1 µm prefiltration, 0.04 µm final filtration, a second 0.1 µm prefilter, and a second 0.04 µm final polish — with mycoplasma control achieved through the 0.1 µm and 0.04 µm filtration stages (not tested per lot).
Sodium pyruvate is excluded by design to provide a pyruvate-free carbon-source environment, enabling researchers to study Warburg-effect glycolysis or run isotope-tracing metabolic flux experiments without confounding contributions from exogenous pyruvate. Sodium pyruvate can be added back as a supplement for cell types that require it — contact us for a custom formulation. The standard formulation also contains 265 mg/L calcium (as calcium chloride dihydrate), which promotes adherent growth; a calcium-free variant to support suspension culture is available through Diagnocine's customization program.
Yes. This formulation uses Earle's salts, which rely on a sodium bicarbonate buffering system (2200 mg/L NaHCO3) designed for use in an approximately 5% CO2 incubator atmosphere, where dissolved CO2 helps maintain the target pH of 7.4. This differs from Hank's salts, which use a phosphate-bicarbonate buffer suited to atmospheric-CO2, open-system conditions. A Hank's salts variant is available on request for applications outside a CO2 incubator.
Yes. This base medium contains no serum, protein, or growth factors, so supplementation (commonly with fetal bovine serum) is expected before use. When filtering serum or other protein-containing supplements into the medium, use a 0.2 µm low-protein-binding PES or PVDF membrane; a 0.1 µm membrane is appropriate only for defined, protein-free additions. Do not use a 0.04 µm membrane to filter serum-containing supplements — it retains immunoglobulins, lipoproteins, and much of the functional serum fraction.
Every batch is tested by LAL assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) and must meet a release specification of less than 0.05 EU/mL before it ships. Endotoxin is controlled per manufacturing batch rather than per unit; results for the released batch are reported on the Certificate of Analysis.
Yes. A CoA is available on request for every lot, listing measured results for endotoxin, sterility, pH, osmolality, appearance, and cultural-response/growth-promotion testing, along with the lot number and expiry date. Contact support@diagnocine.com to request a CoA for your lot.
Scientific References

Supporting literature

The following publications provide foundational context for MEM formulation, Earle's salt buffering, microfluidic cell culture media requirements, and USP-grade media quality standards referenced in this product description.

  1. Eagle H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955;89(3):362–364. doi:10.3181/00379727-89-21807
  2. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130(3373):432–437. doi:10.1126/science.130.3373.432
  3. Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324(5930):1029–1033. doi:10.1126/science.1160809
  4. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
  5. Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20:345–361. doi:10.1038/s41573-020-0079-3
  6. Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev. 1998;62(4):1094–1156. doi:10.1128/MMBR.62.4.1094-1156.1998
  7. Uphoff CC, Drexler HG. Detection of mycoplasma contamination in cell cultures. Curr Protoc Mol Biol. 2014;106:24.4.1–24.4.14. doi:10.1002/0471142727.mb2404s106
  8. Hussain SM, et al. Endotoxin testing of biopharmaceuticals: a review of the bacterial endotoxins test. PDA J Pharm Sci Technol. 2019;73(2):102–115.
  9. United States Pharmacopeia. USP <71> Sterility Tests; USP <85> Bacterial Endotoxins Test; USP <788> Particulate Matter in Injections; USP <791> pH; USP <785> Osmolality. USP-NF. Current edition.
  10. Wenger MPE, et al. TEER measurement techniques for in vitro barrier model systems. J Lab Autom. 2015;20(2):107–126. doi:10.1177/2211068214561025

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