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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEM-BRN1X
$34.10
DCP-MEM-BRN1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate Without Phenol Red Without HEPES

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Bicarbonate, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. Formulated without sodium bicarbonate and without phenol red, it reaches a 0.04 µm final pore size across four sequential filtration passes — well beyond the reach of conventional 0.22 µm-filtered media — making it microchannel-safe from the first fill.

  • Quadruple-stage nano-filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II — Polish), four sequential passes reaching a 0.04 µm final pore size
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> Bacterial Endotoxins Test), controlled per manufacturing batch
  • Low-glucose MEM base (1000 mg/L D-Glucose) with 292 mg/L L-Glutamine and 110 mg/L Sodium Pyruvate
  • Formulated without sodium bicarbonate and without phenol red; pale-yellow, clear solution at pH 7.4 (USP <791>)
  • Manufactured under an ISO 13485:2016 quality management system, with final fill and finish at Diagnocine, Totowa, NJ
  • Mycoplasma risk mitigated by 0.1 µm mycoplasma-retentive grade filtration (not tested per lot)
  • USP <788> Method 1 (light obscuration) particulate compliance verified on every lot
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request; contact support@diagnocine.com
DCP-MEM-BRN1X |Size: 500 mL, 1000 mL |Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose w/o Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L)
  • L-Glutamine292 mg/L
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See CoA
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack, expedited courier
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized organisms (0.2–0.3 µm), subvisible debris, and inconsistent endotoxin loads that accumulate inside microchannels — corrupting biosensor readings, triggering inflammation in primary cultures, and shortening device lifetimes. FluxMPS™ is built to address these failure modes at the filtration stage.

filter_alt

Microchannel-safe purity

0.04 µm final filter reaches sub-mycoplasma particle retention; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.

target

Total metabolic control

A defined low-glucose carbon source (1000 mg/L) with 292 mg/L L-glutamine and 110 mg/L sodium pyruvate supports precise metabolic flux experiments and Warburg-pathway research.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm), supporting low trace-metal and organic-carbon (TOC) background for sensitive assay systems.

visibility

Low background for imaging

Ultra-low particulate profile supports confocal microscopy, live-cell biosensors, and TEER measurements without particulate interference. Validate autofluorescence background for your acquisition settings, as this formulation contains riboflavin.

science

Rich, stable nutrient profile

Micro-batch precision locks in amino acid and vitamin concentrations for lot-to-lot reproducibility in long-term perfusion studies.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Bicarbonate, Phenol Red: 1X Liquid is processed through a four-stage serial filtration sequence — two dedicated prefilter + final-filter pairs — reaching a 0.04 µm final pore size, addressing mycoplasma-sized organisms and subvisible particulates that 0.22 µm filtration cannot retain.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates using a 0.1 µm mycoplasma-retentive grade membrane; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates beyond the reach of standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated 0.1 µm mycoplasma-retentive grade membrane, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filtration prior to aseptic fill and finish in a validated laminar-flow workstation.

Performance vs. conventional media

Four sequential filtration passes reaching a 0.04 µm final pore size deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 (light obscuration) compliance verified on every lot.

5×
A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
0.04
µm final filter pore size across four sequential passes
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is mitigated through 0.1 µm mycoplasma-retentive grade filtration; this is a filtration control, not a per-lot mycoplasma test result.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Bicarbonate, Phenol Red 1X Liquid (DCP-MEM-BRN1X) Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish, for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEM-BRN1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Bicarbonate, Phenol Red: 1X Liquid is intended for organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variability are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: reduces particulate-induced blockage risk in precision fluidic systems
  • Extended Perfusion Stability: supports flow-rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation supports microchannel patency and laminar flow integrity in chip-based devices.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined low-glucose carbon source with controlled endotoxin background supports precise metabolic flux analysis.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered formulation supports sensitive iPSC differentiation protocols where endotoxin can cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurements.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

Chemically defined base enables isotope tracing for 13C metabolic flux and NMR metabolomics workflows; the bicarbonate-free, phenol red-free formulation is compatible with Agilent Seahorse XF assays.

13C tracingSeahorse XFNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load supports confocal imaging and biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Bicarbonate, Phenol Red: 1X Liquid undergoes the quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation MEM, Low Glucose, w/o Sodium Bicarbonate, w/o Phenol Red
Appearance Pale-yellow colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> See CoA
Glucose 1000 mg/L (1.0 g/L)
L-Glutamine 292 mg/L
Sodium Pyruvate 110 mg/L
Phenol Red Not added
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100) aseptic fill
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement No bicarbonate buffering system present; CO2 supplementation not required for pH buffering — validate under your incubation conditions
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.00
Sodium phosphate dibasic anhydrous 7558-79-4 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 126.000
L-Cystine dihydrochloride 30189-89-0 31.300
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt 69847-45-6 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
Sodium Pyruvate 113-24-6 110.000
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system with final packaging, testing, and customization at Diagnocine, Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

filter_alt

Quadruple-Stage Filtration

Two prefilter + final-filter pairs (0.1 µm ×2 + 0.04 µm ×2) reduce particulate and bioburden risk ahead of aseptic fill.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in a validated ISO Class 5 laminar-flow workstation; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay per batch. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per lot.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Lot-specific result reported on the CoA.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com.
Product Comparison

How DCP-MEM-BRN1X (FluxMPS™) compares

Comparison of DCP-MEM-BRN1X release specifications against published supplier specifications for classical liquid media of similar formulation class.

Parameter DCP-MEM-BRN1X (FluxMPS™) Comparison
Grade Microfluidics Suitable (0.04 µm final cut-off) Not specified
Base formulation MEM, Low Glucose, w/o Sodium Bicarbonate, w/o Phenol Red Not specified
Final filtration pore size 0.04 µm Not specified
Number of filtration stages 4 stages (0.1 µm ×2 + 0.04 µm ×2) Not specified
Mycoplasma-retentive filtration check_circle 0.1 µm grade membrane Not specified
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 Not specified
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Not specified
Manufacturing QMS ISO 13485:2016 Not specified
Microfluidic channel compatibility check_circle Validated Not specified
Custom formulation check_circle On request Not specified

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Minimum Essential Medium (MEM), Low Glucose w/o Sodium Bicarbonate, Phenol Red: 1X Liquid.

Yes. DCP-MEM-BRN1X is processed through our Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), reaching a 0.04 µm final pore size that delivers ultra-low particulate counts and reduces microchannel clogging risk in OoC, ToC, and MPS devices.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm) or fine subvisible particulates that accumulate inside microchannels over time. FluxMPS™ uses four sequential passes reaching a 0.04 µm final pore size, delivering approximately 5× cleaner media by particulate count with USP <788> Method 1 (light obscuration) compliance verified on every lot.
This MEM base is supplied without sodium bicarbonate and without phenol red so researchers can define their own buffering strategy (e.g., HEPES) and avoid phenol red interference in fluorescence, absorbance, or biosensor assays. It already contains 1000 mg/L glucose, 292 mg/L L-glutamine, and 110 mg/L sodium pyruvate; contact support@diagnocine.com for a custom buffer or nutrient profile.
This formulation contains no sodium bicarbonate, so it does not rely on a bicarbonate/CO2 buffering system. Validate pH stability under your specific incubation conditions; a HEPES-supplemented or CO2-independent protocol may be appropriate depending on your application.
Yes. Serum, growth factors, and other protein-containing supplements can be added per standard practice. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane immediately before use; do not use a 0.04 µm membrane for serum, as it retains immunoglobulins and lipoproteins and will strip the serum of activity.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. Lot-specific results are reported on the Certificate of Analysis, available from support@diagnocine.com.
Yes. A lot-specific CoA is available for every shipment and includes appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com with your lot number.
Scientific References

Supporting literature

Peer-reviewed publications supporting Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  5. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  6. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  7. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  8. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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