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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-MEM-PBR1X
$44.00
DCP-MEM-PBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains Calcium Contains Magnesium Contains Glucose Without Sodium Bicarbonate Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-MEM-PBR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA formulation engineered for primary fibroblasts, neurons, epithelial, and vascular cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The 0.04 µm final cut-off is five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine | [-] Sodium Pyruvate, [-] Sodium Bicarbonate, [-] Phenol Red.

  • Low Glucose (1.0 g/L) — a physiological carbon source better tolerated by primary cells sensitive to high-glucose stress.
  • NEAA pre-loaded — reduces de novo amino acid synthesis burden and ammonia accumulation in primary cell culture.
  • Quadruple-stage filtration train: 0.1 µm prefiltration I → 0.04 µm final filtration I → 0.1 µm prefiltration II → 0.04 µm final filtration II, reaching a 0.04 µm final cut-off.
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) — a specification relevant to TLR4-sensitive primary fibroblast, neuronal, and epithelial cultures.
  • Bicarbonate-free, phenol-red-free, CO₂-independent base — supplement with HEPES (15–25 mM) for pH buffering in open-chip or imaging workflows.
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ.
  • MEM with Earle's Salts + NEAA is the established base for primary fibroblast, neuronal, epithelial and vascular cell models.
  • pH, glucose concentration, salts, and nutrient composition available on request — contact support@diagnocine.com.
DCP-MEM-PBR1X | Sizes: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • L-Glutamine292 mg/L (Present)
  • Sodium PyruvateNot added
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235–275 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protected from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (gel packs), 2–8°C in transit
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes mycoplasma-sized particles, subvisible particulates, and endotoxin that can alter primary cell phenotype. FluxMPS™ is built on a quadruple-stage filtration train while preserving the full Earle's Salts + NEAA nutritional profile primary fibroblasts, neurons, epithelial, and vascular cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> (Method 1) particulate compliance helps prevent chip microchannel clogging in sub-100 µm geometries.

target

Total metabolic control

A defined Low Glucose (1.0 g/L) carbon source, with sodium pyruvate withheld, lets you set the exact carbon substrate profile for primary cells sensitive to high-glucose stress.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) with controlled trace-metal and total organic carbon (TOC) content supports consistent primary cell performance lot to lot.

visibility

Low background for imaging

An ultra-low particulate baseline and phenol-red-free formulation support confocal, TEER sensor, and biosensor workflows on primary cell chips.

science

Rich, stable nutrient profile

Pre-loaded NEAA (20 amino acid components) and Eagle's vitamin complement, released per micro-batch lot, reduce de novo synthesis burden on primary cells.

tune

Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and material in the mycoplasma size range (0.2–0.3 µm) that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

5×
Finer final cut-off than 0.22 µm conventional filtration (pore-size ratio)
4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled by the 0.1 µm / 0.04 µm barrier filtration train described above (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEM-PBR1X Minimum Essential Medium (MEM) Low Glucose NEAA without Sodium Pyruvate Sodium Bicarbonate Phenol Red 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM Low Glucose + NEAA for organ-on-a-chip and microphysiological systems | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEM-PBR1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEM-PBR1X is purpose-built for primary fibroblast, neuronal, epithelial and vascular cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic liquid-handling systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates beyond the 0.04 µm cut-off.
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries.
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture.

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically conservative base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s Salts ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate accumulation in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate baseline and a phenol-red-free formulation suit confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Sodium Bicarbonate, [-] Phenol Red, [-] Sodium Pyruvate
Media family MEM Low Glucose + NEAA (Earle's Salts base)
Appearance Pale yellow-colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
NEAA Present (20 amino acid components; see Full Composition)
pH USP <791> 7.4
Osmolality USP <785> 235–275 mOsm/kg H2O
Total ingredients 34
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm barrier filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA: 34 ingredients verified per lot with CAS numbers. NEAA is listed under AMINO ACIDS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
Custom formulation: Contact support@diagnocine.com for DCP-MEM-PBR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016–certified quality management system. Final QC at Diagnocine's R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

Type 1 water (18.2 MΩ·cm) with controlled trace-metal and total organic carbon (TOC) content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; a Certificate of Analysis is issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm); Light Obscuration Particle Count Test.

Osmolality — USP <785>

Target: 235–275 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEM-PBR1X compares

FluxMPS™ DCP-MEM-PBR1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations. Rows without a published, attributable competitor figure are shown as "Not specified."

Parameter DCP-MEM-PBR1X (FluxMPS™) Conventional MEM Low Glucose
(0.22 µm filtered)		 Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
Low Glucose MEM + NEAA, without Pyruvate/Bicarbonate/Phenol Red check_circle Yes Not specified Not specified
Final filtration pore size 0.04 µm Not specified Not specified
Number of filtration stages 4 (Quadruple-stage) Not specified Not specified
Mycoplasma barrier filtration check_circle Yes (0.1/0.04 µm barrier stages) Not specified Not specified
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes Not specified Not specified
Water quality Type 1, 18.2 MΩ·cm Not specified Not specified
Manufacturing QMS ISO 13485:2016 Not specified Not specified
Microfluidic channel compatible check_circle Yes Not specified Not specified
Custom formulation available check_circle Yes Not specified Not specified

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified."

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-PBR1X.

Yes. DCP-MEM-PBR1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final cut-off, giving an ultra-low particulate baseline for MPS, OoC, and LoC platforms. MEM with Earle's Salts and NEAA is well suited to primary neuronal, epithelial, fibroblast, and vascular OoC models.
FluxMPS™ uses four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
This formulation is CO₂-independent (add HEPES 15–25 mM for pH buffering), free of phenol red for low-background imaging, and leaves the secondary carbon source to the researcher (add sodium pyruvate at 1 mM fresh, if desired). L-Glutamine is already included. This is a clean, CO₂-free primary cell MEM base suited to open-top chips with confocal or biosensor detection.
No. This formulation is CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering.
Yes. Serum and other protein-containing supplements should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; defined, protein-free additions may use 0.1 µm. Do not use a 0.04 µm membrane for supplement filtration — it will retain serum proteins, lipoproteins, and growth factors.
The release specification is < 0.05 EU/mL, verified by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) as part of batch-level release testing, not a per-unit certificate. A Certificate of Analysis for the relevant batch is available on request.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration statement, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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