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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-MEM-PB1X
$44.00
DCP-MEM-PB1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains Low Glucose Without Sodium Bicarbonate Without Sodium Pyruvate

FluxMPS™ DCP-MEM-PB1X is a Microfluidics Suitable, ultra-filtered MEM Low Glucose + NEAA formulation engineered for primary fibroblasts and related primary cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. NEAA is pre-loaded to reduce metabolic burden on primary cells. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate.

  • Low Glucose (1.0 g/L) — physiological carbon source for primary cells sensitive to high-glucose stress.
  • NEAA pre-loaded — reduces de novo synthesis burden and ammonia accumulation in primary fibroblasts, neurons and epithelial cells.
  • Sodium bicarbonate excluded — CO₂-independent, HEPES-compatible formulation for open-well and atmospheric-incubation chip platforms.
  • Sodium pyruvate excluded — enables researcher-defined, fresh pyruvate addition for precise oxidative-metabolism control.
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II (Polish).
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) — below the TLR4 activation threshold relevant to primary fibroblast and neuronal cultures.
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ.
DCP-MEM-PB1X | Sizes: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
  • Media familyMEM Low Glucose + NEAA
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • NEAAPresent
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate
  • AppearanceOrange-to-Red colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235–275 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • GradeMicrofluidics Suitable (0.04 µm final)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is the primary cell biologist's medium — but conventional 0.22 µm filtered MEM passes particulates and endotoxin that can alter primary cell phenotype. FluxMPS™ addresses these failure modes while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Low-particulate primary cell media helps prevent chip channel clogging.

biotech

Primary cell–optimized formulation

MEM + Earle's Salts + NEAA: an established standard base for primary fibroblasts, neurons, epithelial, and vascular cells.

water_drop

Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) minimizes trace-metal and organic-carbon (TOC) contaminants that can confound sensitive primary-cell assays.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin release specification — below the TLR4 activation threshold. For primary cells, this reduces the risk of LPS-driven fibroblast activation, neuroinflammatory signaling, and epithelial barrier disruption.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation.

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Customization on demand

pH, glucose, HEPES concentration, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — a repeated prefilter-plus-final-filter pair, run twice in series — reaching a final 0.04 µm polish under aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate matter, cell debris and protein aggregates; this mycoplasma-retentive grade protects the first 0.04 µm final filter.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a conventional 0.22 µm filter allows through.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated 0.1 µm prefilter protecting the second 0.04 µm cartridge from fouling.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate 0.04 µm polishing filter; aseptic fill and finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — final polishing filtration
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma (0.2–0.3 µm) is controlled via 0.1 µm mycoplasma-retentive filtration at Stages 1 and 3 (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEM-PB1X Minimum Essential Medium MEM Low Glucose NEAA without Sodium Pyruvate and Sodium Bicarbonate 1X Liquid - Quadruple-stage filtration system 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish - Microfluidics Suitable MEM Low Glucose plus NEAA for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEM-PB1X
Applications

Primary cell models and OoC applications

FluxMPS™ DCP-MEM-PB1X is purpose-built for primary fibroblasts and related primary cell models, with 0.04 µm filtration purity for microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available on request for automated bioreactor and robotic culture systems — the six-stage line reserved for that grade (see the Grade note above).

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate chip geometries
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically relevant base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts in low-serum or serum-free OoC conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load from 0.04 µm final filtration reduces particulate interference for confocal microscopy and biosensor/TEER measurements on primary cell chips. Note: this SKU contains phenol red; phenol red–free formulations are available on request for autofluorescence-sensitive assays.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate
Appearance Orange-to-Red colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
NEAA Present
L-Glutamine 292.000 mg/L
Sodium Pyruvate Not added / None (excluded by formulation)
Phenol Red Present, 11.000 mg/L (phenol red sodium salt)
pH USP <791> 7.4
Osmolality USP <785> 235–275 mOsm/kg H₂O
Total ingredients 35 (4 categories, presented across 3 tabs)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent (bicarbonate-free); supplement with HEPES (15–25 mM) for pH buffering
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA: 35 ingredients verified per lot with CAS numbers. NEAA listed under AMINO ACIDS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 11.000
Custom formulation: Contact support@diagnocine.com for DCP-MEM-PB1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — minimizes trace-metal and organic-carbon contaminants.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm), light obscuration.

Osmolality — USP <785>

Target: 235–275 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-MEM-PB1X lot at support@diagnocine.com.
Product Comparison

How DCP-MEM-PB1X compares

FluxMPS™ DCP-MEM-PB1X vs. conventional 0.22 µm–filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEM-PB1X (FluxMPS™) Conventional MEM Low Glucose (0.22 µm filtered) Standard DMEM (0.22 µm)
Grade Microfluidics Suitable (0.04 µm) Not specified Not specified
MEM + NEAA without Sodium Pyruvate and Sodium Bicarbonate — CO₂-independent, HEPES-compatible check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm grade) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
NEAA included check_circle Yes Optional add-on cancel No
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available on request cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-PB1X.

Yes. DCP-MEM-PB1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size. MEM Low Glucose + NEAA, with mycoplasma-retentive filtration purity and an endotoxin release specification of < 0.05 EU/mL, is well suited to primary neuronal, epithelial, fibroblast, and vascular OoC platforms where low-particulate media is important.
 
Sodium bicarbonate is removed for CO₂-independent, HEPES-buffered use — add HEPES (15–25 mM) for atmospheric incubation in open-top chips. Sodium pyruvate is removed to allow researcher-defined, fresh addition (typically 1 mM at use) for precise control of oxidative metabolism. L-Glutamine and NEAA are already included, providing full nitrogen support for primary cells in CO₂-independent formats.
No. This formulation is bicarbonate-free and CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering in atmospheric culture.
Yes. Serum, growth factors, and other heat-labile supplements can be added at the point of use. Filter serum-containing or protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — never a 0.04 µm membrane, which retains IgM, VLDL, chylomicrons, and much of the serum lipoprotein fraction and will foul rapidly. For defined, protein-free additions, a 0.1 µm filter is acceptable.
FluxMPS™ DCP-MEM-PB1X is manufactured to a release specification of < 0.05 EU/mL, assayed by LAL per USP <85> (assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification before it ships. For primary cells, endotoxin activates TLR4, which can alter fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA in primary cell OoC applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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