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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Product#: DCP-MEM-PR1X
$44.00
DCP-MEM-PR1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Calcium Contains Magnesium Contains Glucose (Low, 1000 mg/L) Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-MEM-PR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MEM Low Glucose + NEAA formulation engineered for primary fibroblasts, neurons, epithelial and vascular cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Earle's Salts plus pre-loaded non-essential amino acids (NEAA) reduce the de novo synthesis burden on primary cells, while the medium ships without sodium pyruvate or phenol red for fresh, defined supplementation and imaging-clean chip work.

  • Low Glucose (1.0 g/L) — a physiological carbon source for primary cells sensitive to high-glucose media.
  • NEAA pre-loaded (Glycine, L-Alanine, L-Asparagine, L-Aspartic acid, L-Glutamic acid, L-Proline) — reduces metabolic burden and ammonia accumulation from de novo synthesis.
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final cut-off.
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) — controlled per manufacturing batch.
  • Formulated without sodium pyruvate (fresh addition at defined concentration) and without phenol red (autofluorescence-sensitive imaging and biosensor platforms).
  • Manufactured under an ISO 13485:2016 quality management system with final QA at Diagnocine, Totowa, NJ, USA.
  • pH 7.4 (USP <791>); Osmolality 290–330 mOsm/kg H₂O (USP <785>).
DCP-MEM-PR1X | Sizes: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate, Phenol Red: 1X Liquid
  • Media familyMEM Low Glucose + NEAA
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • NEAAPresent (Gly, L-Ala, L-Asn, L-Asp, L-Glu, L-Pro)
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate | [-] Sodium Pyruvate, [-] Phenol Red
  • AppearancePale yellow-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290 – 330 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle's Salts is a foundational medium for primary cell biology — but conventional 0.22 µm filtered MEM allows mycoplasma-sized particles, subvisible particulates, and higher endotoxin loads through to the culture, all of which can alter primary cell phenotype. FluxMPS™ addresses these failure modes through a validated quadruple-stage filtration train while preserving the full nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 (light obscuration) particulate compliance. Ultra-low particulate media helps prevent chip channel clogging and reduces the risk of mycoplasma-driven phenotypic drift in long-term primary cell cultures.

biotech

Primary cell–optimized formulation

MEM with Earle's Salts and pre-loaded NEAA is a long-established base for primary fibroblasts, neurons, epithelial cells, and vascular cells.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) with tightly controlled trace-metal and organic-carbon (TOC) content supports reproducible primary cell culture performance batch to batch.

shield

Low endotoxin release specification

< 0.05 EU/mL release specification (LAL, USP <85>), controlled per manufacturing batch. Lower endotoxin loads reduce the risk of TLR4-mediated fibroblast activation, neuroinflammatory signaling, and epithelial barrier disruption in sensitive primary cell assays.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation from NEAA biosynthesis pathways.

tune

Customization on demand

pH, glucose concentration, HEPES, salts, and other component modifications are available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

DCP-MEM-PR1X is processed through four serial filtration stages — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish. Each 0.04 µm final filter is protected by its own dedicated 0.1 µm prefilter, giving the sterile train full redundancy.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge from fouling.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

The quadruple-stage, paired-filter train used for DCP-MEM-PR1X reaches a 0.04 µm final cut-off across two prefilter/final-filter pairs run in series — a materially finer and more redundant process than the single-pass 0.22 µm filtration typical of conventional MEM.

0.04
µm final pore size across 4 filtration passes
4
Serial filtration stages (2 prefilter + 2 final-filter)
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled by 0.1 µm/0.04 µm mycoplasma-retentive filtration at every production stage (not tested per lot by a dedicated mycoplasma assay).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEM-PR1X Minimum Essential Medium (MEM) Low Glucose NEAA without Sodium Pyruvate Phenol Red 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II - Microfluidics Suitable MEM Low Glucose plus NEAA for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEM-PR1X
Applications

Primary cell models and organ-on-a-chip applications

FluxMPS™ DCP-MEM-PR1X is purpose-built for primary fibroblasts, neurons, epithelial, and vascular cell models, with 0.04 µm filtration purity supporting microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request — a separate, finer-filtered product line from the Microfluidics Suitable medium described on this page (see the Grade note above).

  • Total Particulate Exclusion: 10 nm filtration further reduces nanoparticulate aggregates for the most sensitive automated systems.
  • Valve & Sensor Protection: Helps reduce micro-fouling risk in delicate chip geometries.
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration perfusion culture.

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM with Earle’s Salts, NEAA, and low glucose provides a physiologically balanced base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in compartmentalized chips.

Primary neuronsiPSC-NeuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical base for primary dermal, lung, and cardiac fibroblasts under low-serum or serum-free organ-on-a-chip conditions.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s Salts ionic balance supports vascular smooth muscle cells and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate, phenol-red-free formulation supports confocal, TEER-sensor, and biosensor applications on primary cell chips by minimizing particulate background.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate | [-] Sodium Pyruvate, [-] Phenol Red
Appearance Pale yellow-colored, clear solution
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
NEAA Present
pH USP <791> 7.4
Osmolality USP <785> 290 – 330 mOsm/kg H₂O
Total ingredients 35 (across 3 category tabs)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (release specification, per batch)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1/0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement ≈5.8% CO₂ (derived from 2200 mg/L NaHCO₃ at pH 7.4; validate per system)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM Low Glucose + NEAA: 35 ingredients verified per lot, with CAS numbers where established. NEAA is listed under AMINO ACIDS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
Custom formulation: Contact support@diagnocine.com for DCP-MEM-PR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm, with controlled trace-metal and organic-carbon content.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested, with a Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm); NMT 3/mL (≥25 µm), by light obscuration.

Osmolality — USP <785>

Target: 290 – 330 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request for any DCP-MEM-PR1X lot at support@diagnocine.com.
Product Comparison

How DCP-MEM-PR1X compares

FluxMPS™ DCP-MEM-PR1X vs. conventional 0.22 µm-filtered MEM Low Glucose + NEAA formulations.

Parameter DCP-MEM-PR1X (FluxMPS™) Conventional MEM Low Glucose (0.22 µm filtered) Standard DMEM (0.22 µm)
Grade Microfluidics Suitable Standard grade Standard grade
MEM + NEAA without sodium pyruvate and phenol red — imaging-clean primary cell base with defined carbon source check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1/0.04 µm) cancel No cancel No
NEAA included check_circle Yes Optional add-on cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-PR1X.

Yes. DCP-MEM-PR1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final cut-off, delivering ultra-low particulate levels suitable for MPS, OoC, and LoC platforms. MEM with Earle's Salts and NEAA, filtered to sub-mycoplasma purity, is well suited to primary neuronal, epithelial, fibroblast, and vascular organ-on-a-chip platforms.
FluxMPS™ uses four sequential filters run as two dedicated prefilter/final-filter pairs — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II — reaching a 0.04 µm final cut-off with mycoplasma-retentive filtration at every production stage, compared with a single 0.22 µm pass for conventional media.
Phenol red is excluded to reduce optical/particulate background for autofluorescence-sensitive live-cell imaging, confocal, TEER-sensor, and biosensor applications on primary neuronal or epithelial chips. Sodium pyruvate is excluded so it can be added fresh at a defined concentration matched to your assay. Sodium bicarbonate is included and provides the medium's CO₂ buffering.
Yes. This formulation is sodium-bicarbonate buffered (2200 mg/L NaHCO₃) and requires an approximately 5.8% CO₂ incubator atmosphere to maintain pH 7.4; validate the exact set point for your incubator and vessel geometry.
Yes. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; a 0.04 µm membrane is not recommended for serum, as it retains IgM, VLDL, and much of the lipoprotein fraction serum depends on. Defined, protein-free additions may use a 0.1 µm membrane.
DCP-MEM-PR1X is produced to a release specification of < 0.05 EU/mL by LAL assay (USP <85>), controlled and tested per manufacturing batch rather than per unit. Lower endotoxin loads reduce the risk of TLR4-mediated activation in primary fibroblasts, neuroinflammatory gene expression, and epithelial barrier disruption.
Yes. Each batch's CoA covers appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM Low Glucose + NEAA in primary cell and organ-on-a-chip applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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