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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate: 1X Liquid

Product#: DCP-MEM-P1X
$44.00
DCP-MEM-P1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Without Sodium Pyruvate

FluxMPS™ DCP-MEM-P1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Minimum Essential Medium (MEM) with Earle’s Salts and Non-Essential Amino Acids (NEAA) formulation engineered for primary cell culture on organ-on-a-chip (OoC), neuronal chip, and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] Earle’s Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate.

  • MEM with Earle’s Salts — physiological ionic balance optimized for primary fibroblasts, neurons, epithelial, and vascular cells
  • NEAA included reduces the de novo synthesis burden on primary cells cultured in low-serum conditions
  • Low Glucose (1.0 g/L) — physiological carbon source for primary cell types sensitive to high-glucose-induced ROS and glycation
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final pore size
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine, Totowa, NJ
  • Sodium pyruvate intentionally omitted so researchers can define oxidative-phosphorylation support at time of use
  • Custom formulation modifications (pH, glucose, NEAA levels, HEPES) available on request
DCP-MEM-P1X | Size: 500 mL and 1000 mL Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Pyruvate: 1X Liquid
  • BaseMEM + Earle’s Salts + NEAA
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • Formulation[+] Earle’s Salts, [+] NEAA, [+] Low Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium
    [-] Sodium Pyruvate
  • AppearanceOrange-to-red colored, clear solution (contains phenol red)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290 - 330 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Sterility (USP <71>)No growth / 14 days
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle’s Salts is the primary cell biologist’s medium — but conventional 0.22 µm–filtered MEM passes mycoplasma, subvisible particulates, and endotoxin fragments that cause subtle but significant alterations to primary cell gene expression, activation state, and morphology. FluxMPS™ is built to reduce these failure modes while preserving the full MEM + NEAA nutritional profile that primary cells depend on.

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Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion in neuronal, epithelial, and fibroblast chip architectures.

biotech

Primary cell-optimized formulation

MEM + Earle’s Salts + NEAA: an established base for primary fibroblasts, neurons, epithelial cells, and vascular smooth muscle cells in low-serum or serum-free conditions.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) with controlled trace-metal and organic carbon (TOC) content, reducing feed-water contaminants carried into the finished medium.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin release specification — reduces the risk of LPS-driven inflammatory activation that can confound fibroblast activation state and neuronal inflammatory response assays.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions and reducing ammonia accumulation from NEAA synthesis pathways.

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Customization on demand

pH, glucose, NEAA concentrations, HEPES, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions. For primary cell culture, sub-mycoplasma filtration is especially important — mycoplasma in MEM has been associated with altered cytokine profiles, metabolic shifts, and gene expression changes that can persist undetected for months.

  1. 1

    0.1 µm Prefiltration I — Large Particulate Removal

    Removes large aggregates, cell debris and protein aggregates via 0.1 µm mycoplasma-retentive filtration (not tested per lot); protects downstream 0.04 µm membranes and chip geometries from fouling.

  2. 2

    0.04 µm Final Filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II — Redundant Protection

    Second dedicated 0.1 µm mycoplasma-retentive prefilter, protecting the second 0.04 µm cartridge with full pair redundancy.

  4. 4

    0.04 µm Final Filtration II — Polish

    Ultimate 0.04 µm polishing filter; aseptic fill and finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional MEM

5×
Cleaner than 0.22 µm MEM by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma control by 0.1 µm mycoplasma-retentive filtration (not tested per lot) at every production stage. Mycoplasma organisms are approximately 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEM-P1X Minimum Essential Medium (MEM), Low Glucose, NEAA without Sodium Pyruvate, 1X Liquid - Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, 0.04 micron Final filtration II - Microfluidics Suitable MEM Earle Salts NEAA for organ-on-a-chip | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for sub-mycoplasma purity in primary cell OoC applications.
© Diagnocine® — DCP-MEM-P1X
Applications

Primary cell models & OoC applications

FluxMPS™ DCP-MEM-P1X is built for primary cell OoC platforms where low-glucose, NEAA-supplemented, Earle’s salt–balanced conditions better mimic the physiological microenvironment than DMEM — combined with 0.04 µm filtration purity for particle-free chip perfusion.

Automated Bioreactors & Robotics

Next-Generation Primary Cell Perfusion

An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor perfusion of primary cell cultures where trace particulates cause accelerated chip fouling.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from primary cell perfusion circuits
  • Valve & Sensor Protection: Reduces micro-fouling in delicate neuronal and epithelial chip geometries
  • Extended Perfusion Stability: Consistent NEAA and nutrient delivery over multi-week primary cell culture

Inquiry Required: Contact support@diagnocine.com for the MPS Grade 0.01 µm variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in microfluidic compartmentalized chips.

Primary neuronsiPSC-NeuronsDRG neuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells (intestinal, pulmonary, renal) and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical culture base for primary dermal, lung, and cardiac fibroblasts; NEAA reduces glutamine-driven ammonia accumulation associated with myofibroblast differentiation artefacts.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance and low glucose support vascular smooth muscle cells (VSMCs) and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM helps prevent particulate clogging in sub-100 µm neuronal and epithelial chip microchannels where standard MEM can cause progressive flow reduction.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate MEM supports confocal microscopy, TEER sensor, and biosensor applications on primary cell chips. Note: this formulation contains phenol red (11 mg/L); a phenol red-free variant is available on request for applications requiring minimal optical background.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle’s Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate
Appearance Orange-to-red colored, clear solution
Base MEM + Earle’s Salts + NEAA
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
pH USP <791> 7.4
Osmolality USP <785> 290 - 330 mOsm/kg H2O
Total ingredients 36 components across 3 composition tabs
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 required (Earle’s Salts, sodium bicarbonate buffered)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM with Earle’s Salts and NEAA: 36 ingredients verified per lot with CAS numbers. The AMINO ACIDS tab includes both essential amino acids (EAA) and Non-Essential Amino Acids (NEAA) as one combined group per the source composition table.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 11.000
Custom formulation: NEAA concentrations, HEPES addition, glucose level, and pH adjustments available on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular care for primary cell-grade purity standards.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water with controlled trace-metal and organic carbon (TOC) content, produced under strict process controls.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis available for every lot.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290 - 330 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-MEM-P1X lot at support@diagnocine.com.
Product Comparison

How DCP-MEM-P1X compares

FluxMPS™ DCP-MEM-P1X vs. conventional 0.22 µm-filtered MEM and standard DMEM for primary cell OoC applications.

Parameter DCP-MEM-P1X (FluxMPS™) Conventional MEM (0.22 µm filtered) Standard DMEM HG (0.22 µm filtered)
Grade Microfluidics Suitable Not applicable Not applicable
MEM with Earle’s Salts, NEAA, and L-Glutamine without Sodium Pyruvate — researcher-defined carbon source check_circle Yes cancel No cancel No
Salt formulation Earle’s Salts (5% CO2 optimized) Earle’s Salts Modified Earle’s
NEAA included check_circle Yes (Ala, Asn, Asp, Glu, Pro) Optional add-on cancel Not included
Glucose 1.0 g/L (Low — physiological) 1.0 g/L 4.5 g/L (High)
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stages) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-P1X — MEM with Earle’s Salts and NEAA.

Yes. DCP-MEM-P1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM with Earle’s Salts and NEAA is particularly suited to primary cell OoC models — neuronal chips, epithelial chips, fibroblast chips — where low glucose and NEAA supplementation better mimic the physiological microenvironment than DMEM. This product is Microfluidics Suitable at a 0.04 µm final cut-off.
For primary cell cultures, mycoplasma-retentive filtration at the 0.1 µm stages is especially relevant, since mycoplasma contamination has been associated with altered cytokine profiles, metabolic shifts, and gene expression changes that persist undetected for months.
Sodium pyruvate is omitted so researchers can add it fresh (typically 1 mM / 110 mg/L) at time of use, enabling precise control of oxidative phosphorylation support. Primary cells and neurons are sensitive to pyruvate-driven metabolic shifts — omitting it gives researchers the option to study glycolysis-only (no pyruvate), oxidative metabolism (add pyruvate), or combined glycolytic/OXPHOS states. MEM with NEAA and Earle’s Salts is a standard base for primary fibroblasts, neurons, and epithelial cells where pyruvate timing matters.
Yes. DCP-MEM-P1X uses Earle’s Salts with sodium bicarbonate (2200 mg/L) and is optimized for 5% CO2 incubator use, delivering a target pH of 7.4 under standard tissue culture conditions. This differs from Hanks’ Salts formulations, which omit sodium bicarbonate and are designed for CO2-independent environments; DCP-MEM-P1X requires standard CO2 incubation.
Yes. Serum and other protein-containing additions should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — never a 0.04 µm membrane, which will strip serum lipoproteins and clog rapidly. Defined, protein-free additions may be filtered at 0.1 µm. DCP-MEM-P1X already includes NEAA, which reduces the de novo synthesis burden on primary cells, so separate NEAA supplementation is generally not needed for standard applications.
DCP-MEM-P1X is released to meet < 0.05 EU/mL by LAL assay per USP <85>. Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet the specification, with assay sensitivity of 0.005 EU/mL. This matters for primary cells, where elevated endotoxin can activate TLR4/NF-κB signaling and alter fibroblast activation state, neuronal gene expression, and epithelial barrier function independently of experimental conditions.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM with Earle’s Salts and NEAA for primary cell culture and organ-on-a-chip applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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