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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-MEM-B1X
$44.00
DCP-MEM-B1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-MEM-B1X is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Minimum Essential Medium (MEM) with Earle’s Salts and Non-Essential Amino Acids (NEAA), formulated without sodium bicarbonate for CO2-independent, HEPES-buffered culture. Engineered for primary fibroblasts, neurons, epithelial and vascular cells on microfluidic, organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Phenol Red | [-] Sodium Bicarbonate.

  • MEM with Earle’s Salts — physiological ionic balance formulated for primary fibroblasts, neurons, epithelial, and vascular cells
  • Non-Essential Amino Acids (NEAA) pre-loaded, reducing de novo synthesis burden on primary cells in low-serum conditions
  • Low Glucose (1.0 g/L) — physiological carbon source for primary cell types sensitive to high-glucose-induced ROS and glycation
  • Sodium bicarbonate excluded; CO2-independent formulation intended for HEPES-buffered (15–25 mM) atmospheric-incubation and multi-compartment chip use
  • Quadruple-stage filtration to a 0.04 µm final cut-off (0.1 µm ×2 + 0.04 µm ×2), reducing particulate load versus conventional 0.22 µm–filtered MEM
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch before release
  • Contains phenol red sodium salt (11 mg/L) as a pH indicator — a phenol-red-free formulation is available on request for optical/biosensor assays
  • Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine, Totowa, NJ, USA
DCP-MEM-B1X | Sizes: 500 mL, 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • L-Glutamine292 mg/L
  • Sodium Pyruvate110 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235–275 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • FiltrationQuadruple-stage: 0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack, 2–8°C
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle’s Salts is a foundational medium for primary cell biology, but conventional 0.22 µm–filtered MEM passes mycoplasma, subvisible particulates, and endotoxin fragments that can alter primary cell gene expression, activation state, and morphology. FluxMPS™ is built to reduce these risk factors while preserving the full MEM + NEAA nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> particulate compliance support safe perfusion in neuronal, epithelial, and fibroblast chip architectures.

biotech

Primary cell–optimized formulation

MEM + Earle’s Salts + NEAA is a well-established base for primary fibroblasts, neurons, epithelial cells, and vascular smooth muscle cells in low-serum or serum-free conditions.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) supports low trace-metal and organic-carbon background during formulation.

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Low endotoxin release specification

< 0.05 EU/mL endotoxin release specification (LAL, USP <85>), tested per batch — set below levels commonly associated with LPS/TLR4 activation in sensitive primary cultures.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions.

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Customization on demand

pH, glucose, NEAA concentration, HEPES, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reach a final 0.04 µm polish. For primary cell culture, sub-mycoplasma-grade filtration is especially valuable — mycoplasma contamination in MEM has been associated with altered cytokine profiles and gene expression changes that can go undetected for months.

  1. 1

    0.1 µm Prefiltration I — Large Particulate Removal

    Removes large aggregates and particulate contaminants; protects the first 0.04 µm cartridge from fouling.

  2. 2

    0.04 µm Final filtration I — Mycoplasma-retentive Pass

    Retains particulates in the mycoplasma size range (0.2–0.3 µm) that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge downstream.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Filtration architecture

FluxMPS™ DCP-MEM-B1X is processed through two dedicated prefilter + final-filter pairs (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm), giving full redundancy at each 0.04 µm final-filtration stage — valuable for long-duration primary cell OoC experiments.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final pore size
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEM-B1X Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II ? Microfluidics Suitable MEM Earle Salts NEAA for organ-on-a-chip applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for sub-mycoplasma purity in primary cell OoC applications.
© Diagnocine® — DCP-MEM-B1X
Applications

Primary cell models & OoC applications

FluxMPS™ DCP-MEM-B1X is formulated for primary cell OoC platforms where low-glucose, NEAA-supplemented, Earle’s-salt-balanced conditions are often preferred over DMEM — combined with 0.04 µm filtration for particle-controlled chip perfusion.

Automated Bioreactors & Robotics

Next-Generation Primary Cell Perfusion

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor perfusion of primary cell cultures where trace particulates can accelerate chip fouling.

  • Total Particulate Exclusion: 10 nm filtration targets nanoparticulate aggregates in primary cell perfusion circuits
  • Valve & Sensor Protection: Reduces micro-fouling in delicate neuronal and epithelial chip geometries
  • Extended Perfusion Stability: Consistent NEAA and nutrient delivery over multi-week primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically relevant base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in microfluidic compartmentalized chips.

Primary neuronsiPSC-NeuronsDRG neuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells (intestinal, pulmonary, renal) and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical culture base for primary dermal, lung, and cardiac fibroblasts; pre-loaded NEAA reduces glutamine-driven ammonia accumulation associated with myofibroblast differentiation artefacts.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance and low glucose support vascular smooth muscle cells (VSMCs) and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM reduces particulate load in sub-100 µm neuronal and epithelial chip microchannels versus conventional 0.22 µm filtered MEM.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

This formulation contains phenol red sodium salt (11 mg/L) as a pH indicator. For confocal, TEER-sensor and biosensor applications requiring minimal optical background, request the phenol-red-free variant.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Phenol Red | [-] Sodium Bicarbonate
Appearance Orange-to-red colored, clear solution
Base MEM + Earle’s Salts + NEAA
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
pH USP <791> 7.4
Osmolality USP <785> 235–275 mOsm/kg H2O
Total ingredients 36 (across 4 category headings)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent; supplement with HEPES (15–25 mM) for pH buffering in atmospheric incubation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM with Earle’s Salts and NEAA: 36 ingredients released per lot with CAS numbers. The Amino Acids tab includes both essential amino acids (EAA) and Non-Essential Amino Acids (NEAA) as one combined group per the source composition table.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
OTHERS
i-Inositol 87-89-8 2.000
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 11.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: NEAA concentrations, HEPES addition, glucose level, phenol red exclusion, and pH adjustments available on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular care for primary cell–grade purity standards.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water supports low trace-metal and organic-carbon background in the finished formulation.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 235–275 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEM-B1X compares

FluxMPS™ DCP-MEM-B1X vs. conventional 0.22 µm-filtered MEM and standard DMEM for primary cell OoC applications.

Parameter DCP-MEM-B1X (FluxMPS™) Conventional MEM
(0.22 µm filtered)		 Standard DMEM HG
(0.22 µm filtered)
Grade Microfluidics Suitable Conventional cell culture grade Conventional cell culture grade
Formulation trait MEM + Earle’s Salts + NEAA, without sodium bicarbonate, CO2-independent (HEPES-compatible) MEM + Earle’s Salts, with sodium bicarbonate DMEM, with sodium bicarbonate, high glucose
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Not specified Not specified
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Higher clogging risk cancel Higher clogging risk
Custom formulation check_circle Available on request cancel Not typically offered cancel Not typically offered

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-B1X — MEM with Earle’s Salts and NEAA.

Yes. DCP-MEM-B1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, for reduced particulate levels on MPS, OoC, and LoC platforms. MEM with Earle’s Salts and NEAA is a formulation of interest for primary cell OoC models — neuronal chips, epithelial chips, fibroblast chips — where low glucose and NEAA supplementation are often preferred over DMEM. Pre-loaded NEAA reduces the metabolic burden on primary cells that would otherwise synthesize these amino acids de novo.
FluxMPS™ uses four sequential filtration passes run as two prefilter + final-filter pairs — 0.1 µm, 0.04 µm, 0.1 µm, 0.04 µm — reaching a 0.04 µm final cut-off versus the 0.22 µm typical of conventional MEM. This is relevant for primary cell culture, where mycoplasma contamination has been associated with altered cytokine profiles and gene expression changes that can persist undetected for extended periods.
Sodium bicarbonate is excluded so the medium can be buffered with HEPES (15–25 mM recommended) for CO2-independent pH control. This is useful for open-top neuronal chips, primary epithelial culture platforms, and multi-compartment OoC systems where CO2 concentration varies between compartments. For reference, MEM with Earle’s Salts traditionally contains sodium bicarbonate and is designed for 5% CO2 incubator use; Hanks’ Salts formulations omit bicarbonate for CO2-independent use. This product uses Earle’s Salts with sodium bicarbonate removed, for HEPES-buffered, CO2-independent applications.
No. Because sodium bicarbonate has been excluded from this formulation, DCP-MEM-B1X is CO2-independent. Diagnocine recommends supplementing with HEPES (15–25 mM) for stable pH buffering in atmospheric (ambient CO2) incubation. Validate final pH and buffering capacity for your specific culture system.
Yes. When adding serum or other protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — a 0.04 µm membrane will strip serum of the lipoproteins and growth factors it depends on and is not recommended for this purpose. Defined, protein-free additions may be filtered at 0.1 µm.
DCP-MEM-B1X is released to meet < 0.05 EU/mL by LAL assay (USP <85>). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification before product ships. A Certificate of Analysis is available for each lot on request.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM with Earle’s Salts and NEAA for primary cell culture and organ-on-a-chip applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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