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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-MEM-B1X
$44.00
DCP-MEM-B1X
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verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ DCP-MEM-B1X is an MPS-grade, ultra-filtered Minimum Essential Medium (MEM) with Earle’s Salts and Non-Essential Amino Acids (NEAA) formulation engineered for primary cell culture on organ-on-a-chip (OoC), neuronal chip, and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate | [-] Sodium Bicarbonate.

  • MEM with Earle’s Salts — physiological ionic balance optimized for primary fibroblasts, neurons, epithelial, and vascular cells
  • NEAA included reduces metabolic burden on primary cells
  • Low Glucose (1.0 g/L) — physiological carbon source for primary cell types sensitive to high glucose-induced ROS and glycation
  • 0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin for sensitive primary cell cultures
  • Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
  • Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
  • Custom formulation modifications available on request
DCP-MEM-B1X | Size: 500 mL and 1000 mL| UNSPSC 41122100 Cell Culture Media
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate: 1X Liquid
  • BaseMEM + Earle’s Salts + NEAA
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] L-Glutamine, [+] Sodium Pyruvate
    [-] Sodium Bicarbonate
  • AppearanceOrange-to-Red colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)235.00–275.00 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Sterility (USP <71>)No growth / 14 days
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Shelf Life12 months / 2–8°C
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle’s Salts is the primary cell biologist’s medium — but conventional 0.22 µm–filtered MEM passes mycoplasma, subvisible particulates, and endotoxin fragments that cause subtle but significant alterations to primary cell gene expression, activation state, and morphology. FluxMPS™ eliminates these failure modes while preserving the full MEM + NEAA nutritional profile that primary cells depend on.

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Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance ensures safe perfusion in neuronal, epithelial, and fibroblast chip architectures.

biotech

Primary cell–optimized formulation

MEM + Earle’s Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial cells, and vascular smooth muscle cells in low-serum or serum-free conditions.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) — eliminates ionic contaminants that alter primary cell ion channel activity, TEER measurements, and electrophysiology.

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Below TLR4 endotoxin threshold

< 0.05 EU/mL endotoxin — prevents LPS-driven inflammatory activation that confounds primary cell phenotype, fibroblast-to-myofibroblast transition, and neuronal inflammatory responses.

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NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, improving viability in low-serum conditions and reducing ammonia accumulation from NEAA synthesis pathways.

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Customization on demand

pH, glucose, NEAA concentrations, HEPES, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions. For primary cell culture, sub-mycoplasma filtration is especially critical — mycoplasma in MEM causes altered cytokine profiles, metabolic shifts, and gene expression changes that can persist undetected for months.

  1. 1

    0.1 µm Pre-filtration I — Large Particulate Removal

    Removes large aggregates and contaminants; protects downstream 0.04 µm membranes and chip geometries from fouling.

  2. 2

    0.04 µm Pre-filtration II — Mycoplasma Barrier

    Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration. Critical for primary cell cultures where mycoplasma causes undetected phenotypic drift.

  3. 3

    0.1 µm Sterile-filtration I — Second-pass Redundancy

    Second-pass sterility redundancy; no breakthrough from Stage 1.

  4. 4

    0.04 µm Sterile-filtration II — Final Polish

    Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Performance vs. conventional MEM

FluxMPS™ DCP-MEM-B1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered MEM, with a confirmed mycoplasma barrier at every production stage — essential for long-duration primary cell OoC experiments.

5×
Cleaner than 0.22 µm MEM by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-negative by 0.04 µm barrier — USP <63>–equivalent assurance every lot.
FluxMPS™ DCP-MEM-B1X Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Sodium Bicarbonate: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Pre-filtration I, 0.04 μm Pre-filtration II mycoplasma barrier, 0.1 μm Sterile-filtration I, 0.04 μm Final Polish ? MPS-grade MEM Earle Salts NEAA for organ-on-a-chip | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for sub-mycoplasma purity in primary cell OoC applications.
© Diagnocine® — DCP-MEM-B1X
Applications

Primary cell models & OoC applications

FluxMPS™ DCP-MEM-B1X is purpose-built for primary cell OoC platforms where low-glucose, NEAA-supplemented, Earle’s salt–balanced conditions better mimic the physiological microenvironment than DMEM — combined with 0.04 µm filtration purity for particle-free chip perfusion.

Automated Bioreactors & Robotics

Next-Generation Primary Cell Perfusion

Optional 0.01 µm (10 nm) ultra-filtered variant available for automated bioreactor perfusion of primary cell cultures where trace particulates cause accelerated chip fouling.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from primary cell perfusion circuits
  • Valve & Sensor Protection: Eliminates micro-fouling in delicate neuronal and epithelial chip geometries
  • Extended Perfusion Stability: Consistent NEAA and nutrient delivery over multi-week primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is the physiological-closest base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in microfluidic compartmentalized chips.

Primary neuronsiPSC-NeuronsDRG neuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells (intestinal, pulmonary, renal) and their barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is the classical culture base for primary dermal, lung, and cardiac fibroblasts — NEAA reduces glutamine-driven ammonia accumulation that promotes myofibroblast differentiation artefacts.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance and low glucose support vascular smooth muscle cells (VSMCs) and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM prevents particulate clogging in sub-100 µm neuronal and epithelial chip microchannels where standard MEM would cause progressive flow reduction.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate MEM; phenol red–free variants eliminate autofluorescence for confocal, TEER sensor, and biosensor applications on primary cell chips.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate | [-] Sodium Bicarbonate
Appearance Orange-to-Red colored, clear solution
Base MEM + Earle’s Salts + NEAA
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
pH USP <791> 7.4
Osmolality USP <785> 235.00–275.00 mOsm/kg H₂O
Total ingredients 36
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma Negative (0.04 µm barrier)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from manufacture
Shipping condition Cold pack
CO₂ requirement CO₂-independent; supplement with HEPES (15–25 mM) for pH buffering in atmospheric incubation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
Regulatory alignment 21 CFR Part 820 (cGMP) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM with Earle’s Salts and NEAA: 36 ingredients verified per lot with CAS numbers. The AMINO ACIDS tab includes both essential amino acids (EAA) and Non-Essential Amino Acids as one combined group per the source composition table.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulphate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 11.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: NEAA concentrations, HEPES addition, glucose level, and pH adjustments available on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system, with particular care for primary cell–grade purity standards.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — eliminates ionic contaminants that alter primary cell electrophysiology, ion channel activity, and TEER measurements.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay < 0.05 EU/mL — below TLR4 activation threshold for primary cell cultures.

Particulate — USP <788> Method 2

Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).

Osmolality — USP <785>

Target: 235.00–275.00 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Certificate of Analysis: Request for any DCP-MEM-B1X lot at support@diagnocine.com.
Product Comparison

How DCP-MEM-B1X compares

FluxMPS™ DCP-MEM-B1X vs. conventional 0.22 µm–filtered MEM and standard DMEM for primary cell OoC applications.

Parameter DCP-MEM-B1X (FluxMPS™) Conventional MEM
(0.22 µm filtered)		 Standard DMEM HG
(0.22 µm filtered)
MEM with Earle's Salts, NEAA, L-Glutamine, and Sodium Pyruvate without Sodium Bicarbonate — CO₂-independent primary cell OoC check_circle Yes cancel No cancel No
Salt formulation Earle’s Salts (5% CO₂ optimized) Earle’s Salts Modified Earle’s
NEAA included check_circle Yes (Ala, Asn, Asp, Glu, Pro) Optional add-on cancel Not included
Glucose 1.0 g/L (Low — physiological) 1.0 g/L 4.5 g/L (High)
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier check_circle Yes (0.04 µm) cancel No cancel No
Endotoxin specification < 0.05 EU/mL NMT 1 EU/mL NMT 1 EU/mL
USP <788> particulate tested check_circle Yes cancel No cancel No
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle MPS-grade cancel Risk of clogging cancel Risk of clogging
FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-B1X — MEM with Earle’s Salts and NEAA.

DCP-MEM-B1X is processed through a Quadruple-stage filtration system reaching 0.04 µm final pore size, delivering ultra-low particulate levels for MPS, OoC, and LoC platforms. MEM with Earle’s Salts and NEAA is particularly suited to primary cell OoC models — neuronal chips, epithelial chips, fibroblast chips — where low glucose and NEAA supplementation better mimic the physiological microenvironment than DMEM.
FluxMPS™ uses four sequential filters — 0.1 µm pre-filtration I, 0.04 µm pre-filtration II (mycoplasma barrier), 0.1 µm sterile-filtration I, and 0.04 µm final polish — resulting in approximately 5× fewer particles. For primary cell cultures, this is especially critical: mycoplasma in MEM causes altered cytokine profiles, metabolic shifts, and gene expression changes that persist undetected for months.
Sodium bicarbonate is removed for CO₂-independent pH buffering via HEPES (15–25 mM recommended). MEM without bicarbonate is particularly valuable for open-top neuronal chips, primary epithelial culture platforms, and any multi-compartment OoC system where CO₂ concentration varies between compartments. Earle's Salts in bicarbonate-free MEM with HEPES supplementation maintains near-physiological osmolality and ionic balance for demanding primary cell types.
MEM with Earle’s Salts (used in DCP-MEM-B1X) contains sodium bicarbonate and is optimized for 5% CO₂ incubator use, delivering pH 7.2–7.4 under standard tissue culture conditions. Hanks’ Salts formulations omit sodium bicarbonate and are designed for CO₂-independent environments. This variant removes sodium bicarbonate, making it suitable for CO₂-independent HEPES-buffered use in open-top chips and atmospheric incubation systems.
NEAA are pre-loaded, providing basal non-essential nitrogen and carbon skeleton support. This reduces the metabolic burden on primary cells that must synthesize these amino acids de novo from glutamine and glucose — improving viability in low-serum conditions, reducing ammonia from de novo NEAA synthesis, and supporting proliferation in primary cell types adapted to NEAA-supplemented environments.
FluxMPS™ DCP-MEM-B1X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>). This is critical for primary cells: endotoxin concentrations above 0.1 EU/mL activate TLR4/NF-κB, altering fibroblast activation state, neuronal inflammatory gene expression, and epithelial barrier function independently of experimental conditions.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma, particulate count (USP <788> Method 2), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM with Earle’s Salts and NEAA for primary cell culture and organ-on-a-chip applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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